FastQCFastQC Report
Thu 26 May 2016
SRR1546343_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546343_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2181179
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118300.5423672243314281No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC53270.24422571462498033No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGCG406.8193913E-945.00000438
ACAACGT253.8919614E-545.027
ACGCTAA453.8562575E-1045.030
CGTTTCA253.8919614E-545.024
CGATACG1100.045.010
TACGTTG502.1827873E-1145.01
CGTTTTT100700.043.6370431
TACGGGA6100.043.155744
CGTAAGG3050.042.7868842
CGTAAAT850.042.3529416
ACGTAAG2000.041.6251
ACGTTCA650.041.53846422
AGTCGAC603.6379788E-1241.25000413
ATAGGGC8050.041.0869564
TCTAGCG556.184564E-1140.9090921
AGGGCGA18950.040.8443266
TGTTACG1050.040.7142871
TACGATG501.0822987E-940.51
CGGGATT4500.040.56
ACGGGAC5450.040.045875
Produced by FastQC (version 0.11.5)