##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546343_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2181179
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.148859401268762	31.0	31.0	33.0	30.0	34.0
2	31.48857704938476	31.0	31.0	34.0	30.0	34.0
3	31.539792928503346	31.0	31.0	34.0	30.0	34.0
4	35.39126637474504	37.0	35.0	37.0	33.0	37.0
5	35.30517577878753	37.0	35.0	37.0	33.0	37.0
6	35.35258408411231	37.0	35.0	37.0	33.0	37.0
7	35.80023785301436	37.0	35.0	37.0	35.0	37.0
8	35.72214843440176	37.0	35.0	37.0	35.0	37.0
9	37.47276633417065	39.0	37.0	39.0	35.0	39.0
10	36.91945915488825	39.0	37.0	39.0	32.0	39.0
11	36.60554131504109	39.0	35.0	39.0	32.0	39.0
12	35.94273922497879	37.0	35.0	39.0	32.0	39.0
13	35.62588352446085	37.0	35.0	39.0	30.0	39.0
14	36.56797768546277	38.0	35.0	41.0	31.0	41.0
15	36.84059355055225	38.0	35.0	41.0	31.0	41.0
16	36.9425104496238	38.0	35.0	41.0	32.0	41.0
17	36.874448176880485	38.0	35.0	41.0	32.0	41.0
18	36.7813132255537	38.0	35.0	40.0	31.0	41.0
19	36.66651384411825	38.0	35.0	40.0	31.0	41.0
20	36.48299795660971	38.0	35.0	40.0	31.0	41.0
21	36.33917023774757	38.0	35.0	40.0	31.0	41.0
22	36.22079664254974	38.0	35.0	40.0	30.0	41.0
23	36.16734252438704	38.0	35.0	40.0	30.0	41.0
24	36.11927265025016	37.0	35.0	40.0	30.0	41.0
25	36.057910423674535	37.0	35.0	40.0	30.0	41.0
26	35.96324419041262	37.0	35.0	40.0	30.0	41.0
27	35.84134910523162	37.0	34.0	40.0	30.0	41.0
28	35.79716382745295	37.0	34.0	40.0	30.0	41.0
29	35.72867701367013	37.0	34.0	40.0	30.0	41.0
30	35.58870546617219	37.0	34.0	40.0	29.0	41.0
31	35.378609916930245	37.0	34.0	40.0	29.0	41.0
32	35.153552734553195	37.0	34.0	40.0	27.0	41.0
33	34.93224581751429	37.0	34.0	40.0	26.0	41.0
34	34.70444241394218	37.0	34.0	40.0	25.0	41.0
35	34.49721457982128	36.0	33.0	40.0	24.0	41.0
36	34.277072170601315	36.0	33.0	40.0	23.0	41.0
37	34.08615019675139	36.0	33.0	40.0	23.0	41.0
38	34.02428915737773	36.0	33.0	40.0	23.0	41.0
39	33.98460007179603	36.0	33.0	40.0	23.0	41.0
40	33.74477748043604	36.0	33.0	40.0	22.0	41.0
41	33.65752604440076	36.0	33.0	40.0	21.0	41.0
42	33.54045678965367	35.0	33.0	40.0	21.0	41.0
43	33.48892777713338	35.0	32.0	40.0	21.0	41.0
44	33.30579883631742	35.0	32.0	40.0	20.0	41.0
45	33.266606271195535	35.0	32.0	40.0	20.0	41.0
46	33.15842762102514	35.0	32.0	40.0	20.0	41.0
47	33.10266786907448	35.0	32.0	39.0	20.0	41.0
48	33.01588040229619	35.0	32.0	39.0	20.0	41.0
49	33.037924443615125	35.0	32.0	39.0	20.0	41.0
50	32.889621163600054	35.0	32.0	39.0	19.0	41.0
51	32.678151586825294	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	9.0
12	3.0
13	12.0
14	32.0
15	114.0
16	323.0
17	725.0
18	1638.0
19	3114.0
20	5505.0
21	8442.0
22	12650.0
23	18051.0
24	25364.0
25	35115.0
26	44476.0
27	50064.0
28	52344.0
29	57259.0
30	66046.0
31	78259.0
32	95779.0
33	121507.0
34	201750.0
35	275180.0
36	156489.0
37	195019.0
38	275817.0
39	400011.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.68172855139353	22.33911109542133	28.966994455750765	16.012165897434368
2	31.65292715545125	26.00391806449631	29.36191848536961	12.98123629468283
3	28.90060834071848	25.15158086521097	33.10255600296904	12.84525479110151
4	25.168498321320715	27.764800596374712	32.497241170944704	14.569459911359866
5	22.772683947534798	32.69772907221278	30.65974869554493	13.869838284707491
6	20.395620900439624	41.38537002235947	27.820687802330756	10.39832127487015
7	85.99514299376622	4.335911908192771	7.978895817353826	1.690049280687188
8	87.22736648390618	3.319168211320575	7.376606871788148	2.0768584329850968
9	83.07222836823571	4.762699439156529	9.319959526476278	2.8451126661314823
10	46.903394907066314	26.48599679347729	15.527611443169038	11.082996856287357
11	39.79806334097293	22.03207531339702	24.108658665794966	14.06120267983508
12	36.18818079579897	21.848092247357968	27.075998806150253	14.887728150692814
13	22.002641690571934	35.205592938497944	27.17117668930427	15.620588681625854
14	17.14769856119099	37.73881006556546	29.033151336960422	16.08034003628313
15	15.563601153321208	23.381299746604935	45.28092375728906	15.774175342784796
16	16.683912691255507	19.258254366102005	43.30300264214904	20.75483030049345
17	17.431214953013942	20.30681571755459	29.863390395744688	32.398578933686785
18	22.18167330604228	23.269433641163793	33.23271496745567	21.31617808533825
19	26.92222875793321	24.67853394884143	27.395092287244648	21.00414500598071
20	29.906486354398243	22.955245763873574	26.142925454536286	20.9953424271919
21	23.371442692232044	26.45986413769801	29.137361032725877	21.03133213734407
22	22.696257391071526	24.02058703114233	27.62217131193726	25.660984265848885
23	20.38668078135724	28.867506976731395	26.448998454505567	24.296813787405803
24	20.56112772037508	24.245694644960363	35.94377169411589	19.249405940548666
25	18.387899388358314	25.42212262267333	33.30854551598012	22.881432472988234
26	18.498573477921802	31.15558145388343	28.431916866978824	21.91392820121595
27	18.56615160883174	30.52816848135802	30.26257817446436	20.643101735345883
28	17.151687229704667	26.948636494299645	36.95262974748977	18.947046528505915
29	17.934795814557173	24.110354996082396	36.23613651149218	21.718712677868254
30	19.331425802283995	27.990458371367044	32.22619509907256	20.451920727276395
31	26.396733142947003	26.572005323726295	26.514192553660198	20.5170689796665
32	27.308029281411567	27.3523631027073	26.839796275317156	18.49981134056398
33	25.311907000755095	28.201720262298508	25.96073041231371	20.52564232463269
34	19.79388211604825	27.313485046390053	29.370491830335794	23.52214100722591
35	21.139438808094155	26.72971819369249	29.60866577204347	22.522177226169884
36	27.299868557326107	25.00482537196626	27.512505851193325	20.182800219514306
37	20.980946543131033	30.03994628593068	28.88502044077996	20.094086730158324
38	21.813248706318923	29.855871526362577	26.010199071236244	22.320680696082256
39	20.887052369383714	29.150794134731722	27.203544505058964	22.758608990825604
40	23.767421197434967	25.830021286652773	26.845114500002065	23.557443015910202
41	18.16801830569614	25.371737028460295	28.38827074715097	28.071973918692596
42	23.13597370963135	26.011207700055795	25.236076452230648	25.616742138082206
43	23.294053353713746	26.216051043953755	26.05865910133923	24.43123650099327
44	21.13875110662628	28.58495336696346	28.308909997758093	21.967385528652166
45	19.46085121853823	32.73463571765545	24.56034098989583	23.244172073910484
46	22.45441570820185	30.119261188559033	27.174248422527448	20.252074680711672
47	22.44465034735801	27.027905550163467	27.63766751834673	22.889776584131795
48	23.78333002472516	23.87135581261327	29.265090118692687	23.08022404396888
49	20.577999329720303	24.135616563335702	31.170389958825023	24.115994148118975
50	19.618472394975377	29.249593912283217	28.343982772619764	22.78795092012164
51	19.5950905450676	31.006946243293193	25.38214424400748	24.015818967631724
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1077.0
1	1828.0
2	2579.0
3	12204.0
4	21829.0
5	16389.0
6	10949.0
7	11380.5
8	11812.0
9	12307.0
10	12802.0
11	12791.5
12	12781.0
13	12389.5
14	11998.0
15	11695.0
16	11392.0
17	10869.5
18	10347.0
19	10099.0
20	9851.0
21	9337.5
22	8824.0
23	9959.5
24	11095.0
25	11420.5
26	14623.5
27	17501.0
28	20174.0
29	22847.0
30	28555.0
31	34263.0
32	41136.5
33	48010.0
34	52837.5
35	57665.0
36	60761.0
37	63857.0
38	74501.0
39	85145.0
40	108766.0
41	132387.0
42	156256.0
43	180125.0
44	185709.0
45	191293.0
46	188121.5
47	184950.0
48	180119.5
49	175289.0
50	169373.0
51	163457.0
52	149103.0
53	134749.0
54	123487.0
55	112225.0
56	102874.0
57	93523.0
58	90248.0
59	86973.0
60	80924.0
61	74875.0
62	65208.5
63	55542.0
64	48136.0
65	40730.0
66	34092.0
67	27454.0
68	22729.0
69	18004.0
70	15076.5
71	12149.0
72	10699.0
73	9249.0
74	6907.0
75	3713.0
76	2861.0
77	2044.0
78	1227.0
79	927.5
80	628.0
81	421.5
82	215.0
83	193.0
84	171.0
85	118.5
86	66.0
87	58.5
88	51.0
89	42.5
90	34.0
91	22.5
92	11.0
93	8.0
94	5.0
95	2.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2181179.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.554055580030035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.48444226989841	16.129053686333723
2	7.003503487414788	2.738937928956859
3	2.232313330142459	1.3095233678894274
4	1.1349496674444683	0.8877147551098296
5	0.6745040738714224	0.6594645074719239
6	0.4822239688363766	0.5657660573189507
7	0.358669940719032	0.49094163689942205
8	0.2814056966918293	0.4402098106919283
9	0.24419781604058888	0.4297551900641662
>10	2.672477516073473	12.143568163903401
>50	0.9982684702853066	14.226695148275784
>100	1.4229104158060033	47.83832498463706
>500	0.008483732184396763	1.0570966785931786
>1k	0.0011782961367217727	0.2923374598515748
>5k	2.3565922734435454E-4	0.2454731476401858
>10k+	2.3565922734435454E-4	0.5451374763625677
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11830	0.5423672243314281	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5327	0.24422571462498033	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.054007488610517525	0.0
2	0.0	0.0	0.0	0.22854612115741074	0.0
3	0.0	0.0	0.0	0.3388075898401736	0.0
4	0.0	0.0	0.0	0.5088532394636112	0.0
5	0.0	0.0	0.0	0.824691600276731	0.0
6	0.0	0.0	0.0	1.1392462516831494	0.0
7	0.0	0.0	0.0	1.3371667341378217	0.0
8	0.0	0.0	0.0	1.842764853320154	0.0
9	0.0	0.0	0.0	2.0790590776822993	0.0
10	0.0	0.0	0.0	2.504333665416731	0.0
11	0.0	0.0	0.0	2.84726746406416	0.0
12	0.0	0.0	0.0	3.1901095691825385	0.0
13	4.584676452505732E-5	0.0	0.0	3.3326930068554668	0.0
14	4.584676452505732E-5	0.0	0.0	3.389909768982738	0.0
15	4.584676452505732E-5	0.0	0.0	3.4750013639412445	0.0
16	4.584676452505732E-5	0.0	0.0	3.6637066467263804	0.0
17	4.584676452505732E-5	0.0	0.0	3.8741432958963937	0.0
18	4.584676452505732E-5	0.0	0.0	4.235874267999096	0.0
19	4.584676452505732E-5	0.0	0.0	4.367683716008636	0.0
20	4.584676452505732E-5	0.0	0.0	4.53126497183404	0.0
21	4.584676452505732E-5	0.0	0.0	4.687923366216161	0.0
22	4.584676452505732E-5	0.0	0.0	4.835733335044946	0.0
23	4.584676452505732E-5	0.0	0.0	5.001194308215878	0.0
24	4.584676452505732E-5	0.0	0.0	5.122183919797504	0.0
25	4.584676452505732E-5	0.0	0.0	5.220754463526378	0.0
26	4.584676452505732E-5	0.0	0.0	5.314923717860845	0.0
27	4.584676452505732E-5	0.0	0.0	5.42674397653746	0.0
28	4.584676452505732E-5	0.0	0.0	5.5314121399481655	0.0
29	4.584676452505732E-5	0.0	0.0	5.63516336806837	0.0
30	4.584676452505732E-5	0.0	0.0	5.777059104273423	0.0
31	4.584676452505732E-5	0.0	0.0	5.9063928269986095	0.0
32	4.584676452505732E-5	0.0	0.0	6.030133244451739	0.0
33	4.584676452505732E-5	0.0	0.0	6.144887696057958	0.0
34	4.584676452505732E-5	0.0	0.0	6.268811500569187	0.0
35	4.584676452505732E-5	0.0	0.0	6.418363646449925	0.0
36	4.584676452505732E-5	0.0	0.0	6.544442248893832	0.0
37	4.584676452505732E-5	0.0	0.0	6.679552663949176	0.0
38	4.584676452505732E-5	0.0	0.0	6.80677743550621	0.0
39	4.584676452505732E-5	0.0	0.0	6.936294545289497	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTGCG	40	6.8193913E-9	45.000004	38
ACAACGT	25	3.8919614E-5	45.0	27
ACGCTAA	45	3.8562575E-10	45.0	30
CGTTTCA	25	3.8919614E-5	45.0	24
CGATACG	110	0.0	45.0	10
TACGTTG	50	2.1827873E-11	45.0	1
CGTTTTT	10070	0.0	43.637043	1
TACGGGA	610	0.0	43.15574	4
CGTAAGG	305	0.0	42.786884	2
CGTAAAT	85	0.0	42.35294	16
ACGTAAG	200	0.0	41.625	1
ACGTTCA	65	0.0	41.538464	22
AGTCGAC	60	3.6379788E-12	41.250004	13
ATAGGGC	805	0.0	41.086956	4
TCTAGCG	55	6.184564E-11	40.909092	1
AGGGCGA	1895	0.0	40.844326	6
TGTTACG	105	0.0	40.714287	1
TACGATG	50	1.0822987E-9	40.5	1
CGGGATT	450	0.0	40.5	6
ACGGGAC	545	0.0	40.04587	5
>>END_MODULE