FastQCFastQC Report
Thu 26 May 2016
SRR1546342_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546342_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1475810
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71430.4840053936482338No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC42280.2864867428734051No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG16600.11248060387177213No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG15870.10753416767741106No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGG600.045.0000042
CGGGTAT207.033939E-445.06
CGTATCT207.033939E-445.036
ACGTTCA207.033939E-445.039
GCTATGT207.033939E-445.015
TCTAACG207.033939E-445.012
TATTACG207.033939E-445.01
GCGATCG207.033939E-445.09
TCATCGG253.891346E-545.016
TACTACG502.1827873E-1145.01
CGGTCTA1800.045.031
CTTTACG207.033939E-445.01
CACGTAT207.033939E-445.043
TATACGA253.891346E-545.024
ATCATCG207.033939E-445.025
TCGAACG253.891346E-545.026
TACGTTA253.891346E-545.022
CTAGCGA207.033939E-445.035
TCACGTA253.891346E-545.015
TAAGTAG1700.043.6764681
Produced by FastQC (version 0.11.5)