FastQCFastQC Report
Thu 26 May 2016
SRR1546341_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546341_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2161810
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87110.4029493803803294No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC48990.22661566002562666No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG40680.18817564910884862No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG32670.151123364217947No Hit
GTACAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG23760.109907901249416No Hit
GTACGAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG22570.10440325468010603No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC406.8193913E-945.00000410
GCGTGTA253.8919497E-545.00000414
CGCATCG1700.043.67646821
CGTTTTT80350.043.3758541
CGACGGT5350.042.47663528
CGGGATA2200.041.931826
ATAACGG1750.041.1428572
CTAACGG1100.040.9090922
TCACGAC5800.040.73275825
TATAGCG1500.040.51
TCGATAG950.040.2631571
TACGGGA5550.040.1351364
TCGTTAG900.040.0000041
CGTAAGG2200.039.8863642
TAGCCGT2550.039.70588344
TTATACG850.039.7058831
CGGTCTA5650.039.4247831
CGAATAA403.4600998E-739.37500435
ACGTAAG403.4600998E-739.3750041
CGTTTCA403.4600998E-739.37500427