##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546341_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2161810
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17600482928657	31.0	31.0	33.0	30.0	34.0
2	31.512713883273737	31.0	31.0	34.0	30.0	34.0
3	31.61404841313529	31.0	31.0	34.0	30.0	34.0
4	35.465893857462035	37.0	35.0	37.0	33.0	37.0
5	35.387632585657386	37.0	35.0	37.0	33.0	37.0
6	35.43443364588007	37.0	35.0	37.0	33.0	37.0
7	35.829017351201074	37.0	35.0	37.0	35.0	37.0
8	35.73482776007142	37.0	35.0	37.0	35.0	37.0
9	37.52264121268752	39.0	37.0	39.0	35.0	39.0
10	36.94543507523788	39.0	37.0	39.0	32.0	39.0
11	36.701140248217925	39.0	35.0	39.0	32.0	39.0
12	36.08754330861639	37.0	35.0	39.0	32.0	39.0
13	35.80853775308653	37.0	35.0	39.0	31.0	39.0
14	36.73413898538725	39.0	35.0	41.0	31.0	41.0
15	36.98484140604401	39.0	35.0	41.0	32.0	41.0
16	37.06205540727446	38.0	35.0	41.0	32.0	41.0
17	36.99700066148274	38.0	35.0	41.0	32.0	41.0
18	36.932494992621926	38.0	35.0	40.0	32.0	41.0
19	36.85404591522844	38.0	35.0	40.0	31.0	41.0
20	36.703689038352124	38.0	35.0	40.0	31.0	41.0
21	36.54161836609138	38.0	35.0	40.0	31.0	41.0
22	36.433357695634676	38.0	35.0	40.0	31.0	41.0
23	36.40012165731493	38.0	35.0	40.0	31.0	41.0
24	36.38388202478479	38.0	35.0	40.0	31.0	41.0
25	36.31791508041872	38.0	35.0	40.0	31.0	41.0
26	36.22345071953595	38.0	35.0	40.0	31.0	41.0
27	36.123661191316536	38.0	35.0	40.0	30.0	41.0
28	36.080731424130704	38.0	35.0	40.0	30.0	41.0
29	35.983593377771406	38.0	35.0	40.0	30.0	41.0
30	35.83407422483937	37.0	35.0	40.0	30.0	41.0
31	35.65079401057447	37.0	34.0	40.0	29.0	41.0
32	35.50271346695593	37.0	34.0	40.0	29.0	41.0
33	35.32274806759151	37.0	34.0	40.0	27.0	41.0
34	35.118441491157874	37.0	34.0	40.0	27.0	41.0
35	34.94190701310476	37.0	34.0	40.0	26.0	41.0
36	34.735464726317296	37.0	34.0	40.0	25.0	41.0
37	34.55736119270426	37.0	33.0	40.0	24.0	41.0
38	34.460039503934205	37.0	33.0	40.0	24.0	41.0
39	34.42826936687313	36.0	33.0	40.0	24.0	41.0
40	34.23440450363353	36.0	33.0	40.0	23.0	41.0
41	34.1143241080391	36.0	33.0	40.0	23.0	41.0
42	34.010495834509044	36.0	33.0	40.0	23.0	41.0
43	33.97917115750228	36.0	33.0	40.0	23.0	41.0
44	33.776529852299696	35.0	33.0	40.0	23.0	41.0
45	33.73880868346432	35.0	33.0	40.0	23.0	41.0
46	33.63652032324765	35.0	33.0	40.0	23.0	41.0
47	33.553004658133695	35.0	32.0	40.0	23.0	41.0
48	33.482504475416434	35.0	32.0	39.0	22.0	41.0
49	33.470317465457185	35.0	32.0	39.0	22.0	41.0
50	33.324093236685925	35.0	32.0	39.0	22.0	41.0
51	33.12230399526323	35.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	2.0
12	6.0
13	18.0
14	34.0
15	86.0
16	221.0
17	499.0
18	1227.0
19	2345.0
20	4084.0
21	6590.0
22	10016.0
23	14205.0
24	20739.0
25	28626.0
26	37701.0
27	43310.0
28	47405.0
29	52848.0
30	62423.0
31	75231.0
32	93168.0
33	121358.0
34	198921.0
35	264928.0
36	168083.0
37	211602.0
38	294310.0
39	401757.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.06173530513782	23.86893390260939	28.72875044522877	13.340580347024023
2	31.289845083517974	24.897285145318044	31.12752739602463	12.68534237513935
3	29.946572548003758	25.14892613134364	31.421771571044633	13.482729749607966
4	26.703179280325283	27.09724721414	30.699922749917892	15.49965075561682
5	22.760325838070873	32.94156285705034	29.721205841401417	14.576905463477363
6	19.266818082995268	42.17974752637836	27.555751893089585	10.997682497536786
7	88.20567024854174	3.739227776724134	6.460095938125922	1.5950060366082126
8	89.85114325495765	2.650232906684676	5.7708124210730825	1.7278114172845902
9	85.71114020196039	4.018021935322716	7.627358556024813	2.6434793066920776
10	45.5189864049107	23.85972865330441	15.855602481254133	14.76568246053076
11	38.83643798483678	23.24556737178568	21.04727057419477	16.870724069182767
12	35.869988574389055	20.869502870279995	26.09988851934259	17.160620035988362
13	24.5326832607861	31.662495779000004	25.63735943491796	18.167461525295934
14	20.490838695352505	35.28830008187583	27.60062170125959	16.62023952151207
15	18.37622177712195	23.610585574125388	40.547134114468896	17.46605853428377
16	20.22592179701269	18.50384631396839	38.5643974262308	22.705834462788125
17	19.99833472876895	19.094878828389174	29.109912527002834	31.79687391583904
18	23.193157585541748	23.283776095031477	32.104208973036485	21.418857346390293
19	28.445330533210594	24.957281167170102	25.085322021824304	21.512066277794993
20	29.58164686073244	24.31171101993237	24.011730910672075	22.094911208663113
21	24.766191293406912	26.207298513745425	28.32779013881886	20.6987200540288
22	23.413343448314144	23.010717870673187	26.198278294577232	27.377660386435444
23	21.508967023003873	28.446486971565495	24.80879448240132	25.235751523029315
24	22.11276661686272	26.070468727594008	32.41367187680694	19.403092778736337
25	22.22128679208626	24.089489825655352	30.57391722676831	23.115306155490075
26	19.890092098750582	29.034235201058372	26.390802151900488	24.68487054829055
27	20.50503975835064	27.93608133924813	28.70441898224173	22.854459920159496
28	18.304152538844765	26.776404956957368	34.17724036802494	20.74220213617293
29	19.405544428048717	25.794264990910392	32.76546042436662	22.034730156674268
30	22.536208084891825	26.46865358195216	31.01183730300073	19.98330103015529
31	28.752017984929296	25.27756833394239	24.264620850120963	21.70579283100735
32	27.832048144841593	25.91832769762375	25.30430518870761	20.94531896882705
33	25.366197769461703	25.88048903465152	26.324885165671358	22.42842803021542
34	20.734153325222845	27.26294170162965	28.14895851161758	23.853946461529922
35	21.442911264172153	24.59388197852725	29.489178049874877	24.47402870742572
36	28.820710423210183	24.78020732626827	25.945619642799322	20.453462607722233
37	22.63154486286954	28.641277448064358	26.437476003904138	22.28970168516197
38	22.632238725882477	30.1078263122106	23.2565766649243	24.003358296982622
39	22.03403629366133	27.32099490704549	25.092630712227255	25.55233808706593
40	23.841225639626053	24.185289178975026	26.821552310332546	25.151932871066375
41	20.63234049245771	23.00766487341626	27.926598544737974	28.433396089388058
42	22.88091922971954	25.785105999139613	24.373326055481286	26.960648715659563
43	22.322544534441047	25.285617144892473	25.808604826511118	26.583233494155362
44	21.782996655580277	27.08794944976663	27.046132638853553	24.08292125579954
45	21.313667713628856	29.783977315305233	25.121680443702267	23.780674527363644
46	24.163825683108136	27.676067739533078	26.65423880914604	21.505867768212745
47	23.12830452259912	26.814243619929595	26.638835050258812	23.418616807212477
48	22.308528501579694	26.300970020492088	27.583645186209704	23.806856291718514
49	21.55124640925891	24.49299429644603	29.36941729384173	24.586342000453325
50	22.210462529084424	26.640824124229233	27.94584167896346	23.20287166772288
51	21.377179308079803	28.966282883324624	25.640227402038107	24.016310406557466
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	653.0
1	1318.0
2	1983.0
3	9295.5
4	16608.0
5	12494.0
6	8380.0
7	8636.0
8	8892.0
9	9105.5
10	9319.0
11	9284.0
12	9249.0
13	8945.5
14	8642.0
15	8193.0
16	7744.0
17	7397.5
18	7051.0
19	6915.0
20	6779.0
21	6699.0
22	6619.0
23	6687.0
24	6755.0
25	7352.5
26	9111.5
27	10273.0
28	12607.0
29	14941.0
30	18601.0
31	22261.0
32	27982.0
33	33703.0
34	37272.5
35	40842.0
36	44404.5
37	47967.0
38	59015.0
39	70063.0
40	87011.5
41	103960.0
42	127082.5
43	150205.0
44	165808.0
45	181411.0
46	184584.5
47	187758.0
48	187066.5
49	186375.0
50	188064.5
51	189754.0
52	174469.0
53	159184.0
54	143051.0
55	126918.0
56	122952.5
57	118987.0
58	109855.0
59	100723.0
60	94146.0
61	87569.0
62	77460.0
63	67351.0
64	59206.5
65	51062.0
66	43882.5
67	36703.0
68	29966.0
69	23229.0
70	20512.5
71	17796.0
72	14370.0
73	10944.0
74	9276.0
75	5678.5
76	3749.0
77	2652.5
78	1556.0
79	1389.5
80	1223.0
81	924.5
82	626.0
83	411.0
84	196.0
85	166.5
86	137.0
87	101.0
88	65.0
89	44.5
90	24.0
91	21.0
92	18.0
93	10.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2161810.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.02654716500683
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.78627702980747	15.370839101929393
2	7.044512935836968	2.6806551525640554
3	2.3201256288655983	1.3243193911905728
4	1.201483868602756	0.9144035797586082
5	0.7634222510983104	0.7262644733668845
6	0.5820346647439756	0.6644466000252114
7	0.44204865514535596	0.5887461710445667
8	0.335825551316097	0.5111680553064112
9	0.30261760498169993	0.5181991320731147
>10	3.557578230093237	15.931379112114607
>50	1.3077833614975312	17.95724276712661
>100	1.3426321974936992	39.569360831904035
>500	0.009511835717380703	1.25830331582392
>1k	0.003902291576361314	1.580443071630442
>5k	2.4389322352258213E-4	0.40422924414154615
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8711	0.4029493803803294	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4899	0.22661566002562666	No Hit
GTGCAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	4068	0.18817564910884862	No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	3267	0.151123364217947	No Hit
GTACAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	2376	0.109907901249416	No Hit
GTACGAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	2257	0.10440325468010603	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0494955615895939	0.0
2	0.0	0.0	0.0	0.18849945184821978	0.0
3	0.0	0.0	0.0	0.27046780244332297	0.0
4	0.0	0.0	0.0	0.3935591009385654	0.0
5	0.0	0.0	0.0	0.6397416979290502	0.0
6	0.0	0.0	0.0	0.9094231222910432	0.0
7	0.0	0.0	0.0	1.0756727001910436	0.0
8	0.0	0.0	0.0	1.5545769517210115	0.0
9	0.0	0.0	0.0	1.776936918600617	0.0
10	0.0	0.0	0.0	2.146673389428303	0.0
11	0.0	0.0	0.0	2.4266702439159777	0.0
12	0.0	0.0	0.0	2.6815029998010926	0.0
13	0.0	0.0	0.0	2.787340238041271	0.0
14	0.0	0.0	0.0	2.8283244133388226	0.0
15	0.0	0.0	0.0	2.8896156461483664	0.0
16	0.0	0.0	0.0	3.0367608624254676	0.0
17	0.0	0.0	0.0	3.2156850046951395	0.0
18	0.0	0.0	0.0	3.5098829221809504	0.0
19	0.0	0.0	0.0	3.619559535759387	0.0
20	0.0	0.0	0.0	3.745796346579949	1.850301367835286E-4
21	0.0	0.0	0.0	3.8893334751897717	1.850301367835286E-4
22	0.0	0.0	0.0	4.0310665599659545	1.850301367835286E-4
23	0.0	0.0	0.0	4.206012554294781	1.850301367835286E-4
24	0.0	0.0	0.0	4.334007151414787	1.850301367835286E-4
25	0.0	0.0	0.0	4.437115195137408	1.850301367835286E-4
26	0.0	0.0	0.0	4.536476378590163	1.850301367835286E-4
27	0.0	0.0	0.0	4.645181583950486	1.850301367835286E-4
28	0.0	0.0	0.0	4.759807753687882	1.850301367835286E-4
29	0.0	0.0	0.0	4.881280038486269	1.850301367835286E-4
30	0.0	0.0	0.0	5.026528695861338	1.850301367835286E-4
31	0.0	0.0	0.0	5.161045605302964	1.850301367835286E-4
32	0.0	0.0	0.0	5.2807601038019065	1.850301367835286E-4
33	0.0	0.0	0.0	5.421660552962564	1.850301367835286E-4
34	0.0	0.0	0.0	5.562653517191612	1.850301367835286E-4
35	0.0	0.0	0.0	5.727376596463149	1.850301367835286E-4
36	0.0	0.0	0.0	5.859719401797568	1.850301367835286E-4
37	0.0	0.0	0.0	5.998029429043255	1.850301367835286E-4
38	0.0	0.0	0.0	6.162012387767658	1.850301367835286E-4
39	0.0	0.0	0.0	6.361613647822889	1.850301367835286E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAC	40	6.8193913E-9	45.000004	10
GCGTGTA	25	3.8919497E-5	45.000004	14
CGCATCG	170	0.0	43.676468	21
CGTTTTT	8035	0.0	43.375854	1
CGACGGT	535	0.0	42.476635	28
CGGGATA	220	0.0	41.93182	6
ATAACGG	175	0.0	41.142857	2
CTAACGG	110	0.0	40.909092	2
TCACGAC	580	0.0	40.732758	25
TATAGCG	150	0.0	40.5	1
TCGATAG	95	0.0	40.263157	1
TACGGGA	555	0.0	40.135136	4
TCGTTAG	90	0.0	40.000004	1
CGTAAGG	220	0.0	39.886364	2
TAGCCGT	255	0.0	39.705883	44
TTATACG	85	0.0	39.705883	1
CGGTCTA	565	0.0	39.42478	31
CGAATAA	40	3.4600998E-7	39.375004	35
ACGTAAG	40	3.4600998E-7	39.375004	1
CGTTTCA	40	3.4600998E-7	39.375004	27
>>END_MODULE