FastQCFastQC Report
Thu 26 May 2016
SRR1546340_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546340_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1786094
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82660.46279759072030924No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC80150.44874457895273145No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT20330.11382379650791057No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC302.16618E-645.00000437
CGAGTAC302.16618E-645.0000048
ATCGTTA302.16618E-645.00000436
TTGAGTA600.045.00000432
TGCGCAA253.891676E-545.00000413
CACTACG253.891676E-545.0000041
ACTACGC253.891676E-545.00000425
ACGTCGT302.16618E-645.00000445
CTCGAAT253.891676E-545.00000433
ATCGCTA253.891676E-545.0000042
AAATGCG302.16618E-645.0000041
ACGCGTC253.891676E-545.00000428
AATCCCG351.2124292E-745.010
ATTAGCC351.2124292E-745.012
TCGATAG351.2124292E-745.01
ATCGTAT900.045.013
GCCGAAC207.034339E-445.026
CGTAGCA207.034339E-445.026
TGGATCG551.8189894E-1245.034
ATAGTAC351.2124292E-745.033