##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546340_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1786094
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.107811235018985	31.0	31.0	33.0	30.0	34.0
2	31.433513577672844	31.0	31.0	34.0	30.0	34.0
3	31.502097314027147	31.0	31.0	34.0	30.0	34.0
4	35.36534023405263	37.0	35.0	37.0	33.0	37.0
5	35.256981435467566	37.0	35.0	37.0	33.0	37.0
6	35.29995957659563	37.0	35.0	37.0	32.0	37.0
7	35.745460205341935	37.0	35.0	37.0	35.0	37.0
8	35.65089351400318	37.0	35.0	37.0	35.0	37.0
9	37.411574082887014	39.0	37.0	39.0	35.0	39.0
10	36.910095997187156	39.0	37.0	39.0	32.0	39.0
11	36.57734587317353	39.0	35.0	39.0	32.0	39.0
12	35.637585143895	37.0	35.0	39.0	31.0	39.0
13	35.17426686389406	37.0	35.0	39.0	30.0	39.0
14	36.01969157278396	38.0	35.0	40.0	30.0	41.0
15	36.36149105254259	38.0	35.0	40.0	31.0	41.0
16	36.54061880281777	38.0	35.0	40.0	31.0	41.0
17	36.475410028811474	38.0	35.0	40.0	31.0	41.0
18	36.4271057402354	38.0	35.0	40.0	31.0	41.0
19	36.27596419897273	37.0	35.0	40.0	31.0	41.0
20	36.09798308487683	37.0	35.0	40.0	31.0	41.0
21	35.911973278002165	36.0	34.0	40.0	30.0	41.0
22	35.76668697168234	36.0	34.0	40.0	30.0	41.0
23	35.75488356155947	36.0	34.0	40.0	30.0	41.0
24	35.73251575784925	36.0	34.0	40.0	30.0	41.0
25	35.66512624755472	36.0	34.0	40.0	30.0	41.0
26	35.532945634440296	36.0	34.0	40.0	30.0	41.0
27	35.39917607919852	36.0	34.0	40.0	29.0	41.0
28	35.370271665433066	36.0	34.0	40.0	29.0	41.0
29	35.312830679684275	36.0	34.0	40.0	29.0	41.0
30	35.18666430770161	36.0	34.0	40.0	29.0	41.0
31	34.95202492142071	36.0	34.0	40.0	27.0	41.0
32	34.7077846966621	35.0	34.0	40.0	26.0	41.0
33	34.48600577573185	35.0	33.0	40.0	25.0	41.0
34	34.258760177235914	35.0	33.0	40.0	24.0	41.0
35	34.07754519079063	35.0	33.0	40.0	23.0	41.0
36	33.82406637052697	35.0	33.0	40.0	23.0	41.0
37	33.620877176677155	35.0	33.0	40.0	23.0	41.0
38	33.548569672144914	35.0	33.0	40.0	23.0	41.0
39	33.52430051273897	35.0	33.0	40.0	22.0	41.0
40	33.25396591668748	35.0	32.0	39.0	21.0	41.0
41	33.171334767375065	35.0	32.0	39.0	21.0	41.0
42	33.050687701767096	35.0	32.0	39.0	20.0	41.0
43	32.98367499134984	35.0	32.0	39.0	20.0	41.0
44	32.74003551884727	35.0	31.0	39.0	18.0	41.0
45	32.73221510178076	35.0	31.0	39.0	19.0	41.0
46	32.661424314733715	35.0	31.0	39.0	19.0	41.0
47	32.59915995462725	35.0	31.0	39.0	19.0	41.0
48	32.53665036666603	35.0	31.0	38.0	18.0	40.0
49	32.56892750325571	35.0	31.0	38.0	18.0	40.0
50	32.38289418138127	35.0	31.0	38.0	18.0	40.0
51	32.15986224689182	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	4.0
12	4.0
13	14.0
14	33.0
15	77.0
16	275.0
17	665.0
18	1472.0
19	2791.0
20	4950.0
21	7595.0
22	11146.0
23	15810.0
24	22151.0
25	30325.0
26	38285.0
27	43279.0
28	46241.0
29	50732.0
30	58394.0
31	70221.0
32	86162.0
33	110235.0
34	189256.0
35	262689.0
36	122090.0
37	148777.0
38	199577.0
39	262786.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.65354790957251	23.174872095197678	28.539091447594583	14.63248854763523
2	30.654769569798678	26.070520364549683	29.875527267881758	13.399182797769882
3	27.951664358090895	25.79668259341334	32.803816596438935	13.447836452056835
4	26.40409743272191	27.62027082561164	31.46060621669408	14.51502552497237
5	22.607824672161712	32.10368547232116	30.397504274691027	14.890985580826094
6	20.33969096811254	41.191449050273945	27.51019823144806	10.958661750165446
7	85.35782551198314	4.938205939888942	7.956412148520739	1.7475563996071874
8	86.31354228836781	4.303076993708058	6.952545610701341	2.4308351072228
9	82.5444797418277	5.1341642713093485	9.114525887215343	3.206830099647611
10	52.678694402422266	21.445623802554625	13.685281961643675	12.19039983337943
11	46.32208607161773	19.84425231818706	19.958524019452504	13.875137590742703
12	41.9810491497088	19.23549376460589	24.329458583926712	14.453998501758585
13	22.988823656537676	38.44758450563072	24.159142799875035	14.404449037956567
14	16.460779779787625	41.735149437823544	26.97786342712086	14.826207355267975
15	14.424660740140215	22.444619376135858	48.26364121933112	14.867078664392805
16	15.973683355971186	17.296234128774856	45.55807253145691	21.172009983797047
17	15.823859214576613	17.300209283497956	29.57307958035803	37.302851921567395
18	21.4193093980496	22.294067389510293	34.27759121300446	22.009031999435642
19	28.09460196383841	24.891355102251055	25.674068666038853	21.339974267871682
20	32.077706996384286	22.454305316517495	23.695001494882128	21.772986192216088
21	23.26338927290501	28.574755863913097	26.328289552509553	21.83356531067234
22	22.135677069627917	24.66919434251501	25.070013112411775	28.125115475445302
23	20.0055540189934	30.126913813046798	23.760171637103085	26.10736053085672
24	20.668173119667834	23.592375317312527	37.04665040025889	18.692801162760748
25	18.943459862694798	23.47620002082757	34.50154359177064	23.078796524706988
26	17.676729220298597	31.84692407006574	27.312280316713455	23.16406639292221
27	18.285655738163836	31.818034213204903	28.410878710750946	21.48543133788031
28	16.073286176427445	27.80363183572645	36.98439163896189	19.138690348884214
29	16.98460439372172	24.276325882064437	35.695377734878456	23.04369198933539
30	19.943799150548628	28.344812759014925	30.560429630243423	21.150958460193024
31	28.813545087772535	26.381982135318744	22.540247041869016	22.2642257350397
32	29.791321173465672	26.068224852667328	24.694165032747435	19.446288941119562
33	28.212736843637572	26.24469932713508	23.85305588619636	21.689507943030996
34	19.552162428181273	27.43349454172065	27.913648441795335	25.10069458830274
35	20.114058946505615	24.975057303814918	30.06980595646142	24.84107779321805
36	29.409762308142795	24.64791886653222	25.286071169826446	20.656247655498536
37	21.232533114158606	30.284184371035344	27.78286025259589	20.700422262210164
38	21.587609610692382	31.636856738783063	23.063175846288043	23.712357804236508
39	21.760164918531725	28.791765718937523	25.673004892239714	23.77506447029104
40	23.97270244455219	24.09531637192667	25.939116306308623	25.992864877212508
41	18.149268739495234	22.898514859800212	27.038778474145257	31.913437926559297
42	22.457160709346766	25.26765108667293	24.064242979372867	28.210945224607443
43	22.875447764787296	24.753232472647017	25.39306441878199	26.978255343783697
44	21.041781675544513	27.52940214792726	28.027304274019173	23.40151190250905
45	18.40071127275496	33.34298194831851	23.411757723837603	24.844549055088926
46	22.489465839983787	30.156979419896153	26.864543523465173	20.48901121665489
47	22.741636218474504	25.911402199436314	27.253772757760792	24.093188824328397
48	22.75484940882171	22.592036029458697	29.949039636211754	24.70407492550784
49	21.037974485105487	21.614763836617783	32.24158414954644	25.10567752873029
50	20.09983797045396	28.93655093180986	27.634547789757985	23.329063307978192
51	19.116631039575744	31.401370812510425	24.22056173975166	25.26143640816217
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1022.0
1	1446.5
2	1871.0
3	8338.5
4	14806.0
5	11238.5
6	7671.0
7	8141.0
8	8611.0
9	9160.5
10	9710.0
11	9759.5
12	9809.0
13	9643.0
14	9477.0
15	9119.0
16	8761.0
17	8330.0
18	7899.0
19	7535.5
20	7172.0
21	7127.0
22	7082.0
23	6900.5
24	6719.0
25	7782.5
26	8520.5
27	8195.0
28	12047.0
29	15899.0
30	16755.5
31	17612.0
32	22234.5
33	26857.0
34	29635.5
35	32414.0
36	35220.5
37	38027.0
38	46377.5
39	54728.0
40	78558.5
41	102389.0
42	129500.5
43	156612.0
44	160421.5
45	164231.0
46	161967.5
47	159704.0
48	157482.0
49	155260.0
50	146706.0
51	138152.0
52	127991.5
53	117831.0
54	102611.5
55	87392.0
56	84732.5
57	82073.0
58	77193.0
59	72313.0
60	69452.0
61	66591.0
62	60107.5
63	53624.0
64	48348.5
65	43073.0
66	35180.0
67	27287.0
68	23341.5
69	19396.0
70	16667.0
71	13938.0
72	12464.0
73	10990.0
74	8565.5
75	4539.0
76	2937.0
77	2177.0
78	1417.0
79	1093.0
80	769.0
81	644.0
82	519.0
83	324.0
84	129.0
85	90.0
86	51.0
87	38.0
88	25.0
89	21.0
90	17.0
91	18.0
92	19.0
93	22.5
94	26.0
95	13.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1786094.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.76399219522114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.45943548818673	16.297276394108636
2	7.235000550460215	2.8598498882343266
3	2.4304083237619465	1.441037134260948
4	1.141617830509522	0.9025170356846193
5	0.6921743481291971	0.6840064207078865
6	0.46528141724320754	0.5517490979385711
7	0.3553815037236842	0.4916630086144592
8	0.28567481576865755	0.4516859863378387
9	0.22785951629040027	0.40530723314133016
>10	2.4716546100051113	11.212285706794695
>50	0.837426417536688	12.101751551923952
>100	1.382408382296862	49.37953303191463
>500	0.012256404214162846	1.5585317937326593
>1k	0.0028503265614332196	0.745634599213673
>5k	5.700653122866439E-4	0.9171711173917959
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8266	0.46279759072030924	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8015	0.44874457895273145	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2033	0.11382379650791057	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08622166582497898	0.0
2	0.0	0.0	0.0	0.2929856995208539	0.0
3	0.0	0.0	0.0	0.41296818644483435	0.0
4	0.0	0.0	0.0	0.6333933152454462	0.0
5	0.0	0.0	0.0	1.0384111922440813	0.0
6	0.0	0.0	0.0	1.4638087357104386	0.0
7	0.0	0.0	0.0	1.728688411696137	0.0
8	0.0	0.0	0.0	2.508826523128122	0.0
9	0.0	0.0	0.0	2.8799716028383724	0.0
10	0.0	0.0	0.0	3.505694549111077	0.0
11	0.0	0.0	0.0	3.884286045415303	0.0
12	0.0	0.0	0.0	4.309515624597585	0.0
13	0.0	0.0	0.0	4.4667301944914435	0.0
14	0.0	0.0	0.0	4.519470979690879	0.0
15	0.0	0.0	0.0	4.613922895435515	0.0
16	0.0	0.0	0.0	4.820742917226081	0.0
17	0.0	0.0	0.0	5.048894403094126	0.0
18	0.0	0.0	0.0	5.486777291676698	0.0
19	0.0	0.0	0.0	5.620980754652331	0.0
20	0.0	0.0	0.0	5.784130062583492	0.0
21	0.0	0.0	0.0	5.937873370606474	0.0
22	0.0	0.0	0.0	6.084618166793013	0.0
23	0.0	0.0	0.0	6.26501180788917	0.0
24	0.0	0.0	0.0	6.394624247100096	0.0
25	5.598809469154479E-5	0.0	0.0	6.495626769923644	0.0
26	5.598809469154479E-5	0.0	0.0	6.5914783880355685	0.0
27	5.598809469154479E-5	0.0	0.0	6.695616244161841	0.0
28	5.598809469154479E-5	0.0	0.0	6.80137775503417	0.0
29	5.598809469154479E-5	0.0	0.0	6.911786277765896	0.0
30	5.598809469154479E-5	0.0	0.0	7.0668733000614745	0.0
31	5.598809469154479E-5	0.0	0.0	7.20006897733266	0.0
32	5.598809469154479E-5	0.0	0.0	7.320723321392939	0.0
33	5.598809469154479E-5	0.0	0.0	7.447368391585213	0.0
34	5.598809469154479E-5	0.0	0.0	7.576924842701448	0.0
35	5.598809469154479E-5	0.0	0.0	7.743545412503485	0.0
36	5.598809469154479E-5	0.0	0.0	7.863471911332774	0.0
37	5.598809469154479E-5	0.0	0.0	7.998179267160631	0.0
38	5.598809469154479E-5	0.0	0.0	8.131318956337125	0.0
39	5.598809469154479E-5	0.0	0.0	8.2725209311492	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	30	2.16618E-6	45.000004	37
CGAGTAC	30	2.16618E-6	45.000004	8
ATCGTTA	30	2.16618E-6	45.000004	36
TTGAGTA	60	0.0	45.000004	32
TGCGCAA	25	3.891676E-5	45.000004	13
CACTACG	25	3.891676E-5	45.000004	1
ACTACGC	25	3.891676E-5	45.000004	25
ACGTCGT	30	2.16618E-6	45.000004	45
CTCGAAT	25	3.891676E-5	45.000004	33
ATCGCTA	25	3.891676E-5	45.000004	2
AAATGCG	30	2.16618E-6	45.000004	1
ACGCGTC	25	3.891676E-5	45.000004	28
AATCCCG	35	1.2124292E-7	45.0	10
ATTAGCC	35	1.2124292E-7	45.0	12
TCGATAG	35	1.2124292E-7	45.0	1
ATCGTAT	90	0.0	45.0	13
GCCGAAC	20	7.034339E-4	45.0	26
CGTAGCA	20	7.034339E-4	45.0	26
TGGATCG	55	1.8189894E-12	45.0	34
ATAGTAC	35	1.2124292E-7	45.0	33
>>END_MODULE