FastQCFastQC Report
Thu 26 May 2016
SRR1546339_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546339_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences650332
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55600.8549479342858725No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCG26210.4030249165041856No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21610.33229181402729685No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGC17230.26494159906017234No Hit
GAATCTGTCTCTTATACACATCTGACGCTAACGCGATCGTATGCCGTCTTC10340.15899571295891946No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7380.11348049919118235No Hit
GTACAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG6820.10486951280269155No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC551.8189894E-1245.025
CTCACGT551.8189894E-1245.045
GATAATC207.031025E-445.09
GGTCGAG207.031025E-445.01
CGACGGT551.8189894E-1245.028
CTATCGC253.888931E-545.044
TAGACCC207.031025E-445.039
AACCACG253.888931E-545.033
TAAACGG406.8066583E-945.02
CACGACC253.888931E-545.027
ACGGGTA1000.045.05
TCGGCGA207.031025E-445.023
ACTAGGT207.031025E-445.03
CTTGCGA207.031025E-445.013
TCTCGAG253.888931E-545.01
CGAATTA207.031025E-445.044
ATGGTCG406.8066583E-945.044
GTATATC207.031025E-445.024
CTCGAGG406.8066583E-945.02
GGTCTAA551.8189894E-1245.032