FastQCFastQC Report
Thu 26 May 2016
SRR1546338_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546338_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2081272
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133810.642924134855992No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACGAGATCGTATGCCG35290.1695597692180551No Hit
GAATCTGTCTCTTATACACATCTGACGCACACGAGATCGTATGCCGTCTTC32990.15850883498168428No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACGAGATCGTATGC24720.1187735192709074No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21610.10383073428172772No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99200.043.593751
CGGTCTA4050.041.66666831
TACGGGA6700.039.6268654
GTACGAG2850.039.4736861
TAGGGAC18000.038.55
ACGGGAT7450.037.751685
CACGACG4650.037.74193626
GCGCGAC14450.037.5259489
TCACGAC4850.037.11340325
GGGCGAT38500.036.9935077
TATGGGA15600.036.923084
GTTTTTT123250.036.8762662
ATGGGCG7900.036.7405055
TAGGGCG9800.036.7346955
CGTAAGG3250.036.6923072
CATATGC12900.036.4534933
CTCACGA5000.036.44999724
CGGGATA3150.036.4285746
GGCGATT10900.036.3302738
ACCCGCT14000.036.32142634
Produced by FastQC (version 0.11.5)