##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546338_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2081272
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.081528027091124	31.0	31.0	33.0	30.0	34.0
2	31.404719805964813	31.0	31.0	34.0	30.0	34.0
3	31.46872297325866	31.0	31.0	34.0	30.0	34.0
4	35.35454375977768	37.0	35.0	37.0	33.0	37.0
5	35.266906968430845	37.0	35.0	37.0	33.0	37.0
6	35.32009031015648	37.0	35.0	37.0	33.0	37.0
7	35.70856668422004	37.0	35.0	37.0	35.0	37.0
8	35.58872747050842	37.0	35.0	37.0	35.0	37.0
9	37.34609796316868	39.0	37.0	39.0	35.0	39.0
10	36.82290733743595	39.0	37.0	39.0	32.0	39.0
11	36.57725227649245	39.0	35.0	39.0	32.0	39.0
12	36.14333349989814	38.0	35.0	39.0	32.0	39.0
13	35.94336876679262	38.0	35.0	39.0	31.0	39.0
14	36.88549502419674	39.0	35.0	41.0	31.0	41.0
15	37.074427081131155	39.0	35.0	41.0	31.0	41.0
16	37.11224866331743	39.0	35.0	41.0	32.0	41.0
17	36.98832781106938	39.0	35.0	41.0	31.0	41.0
18	36.915000057657046	38.0	35.0	40.0	31.0	41.0
19	36.8121663098336	38.0	35.0	40.0	31.0	41.0
20	36.61944570435772	38.0	35.0	40.0	31.0	41.0
21	36.50330519028748	38.0	35.0	40.0	31.0	41.0
22	36.423613540181194	38.0	35.0	40.0	30.0	41.0
23	36.32346180604938	38.0	35.0	40.0	30.0	41.0
24	36.34322616169342	38.0	35.0	40.0	30.0	41.0
25	36.29268111039787	38.0	35.0	40.0	30.0	41.0
26	36.16165018315722	38.0	35.0	40.0	30.0	41.0
27	36.04249949069607	38.0	35.0	40.0	30.0	41.0
28	35.96586654699626	38.0	35.0	40.0	30.0	41.0
29	35.81347752720452	38.0	34.0	40.0	29.0	41.0
30	35.63279859624307	38.0	34.0	40.0	29.0	41.0
31	35.4353736561103	38.0	34.0	40.0	27.0	41.0
32	35.24615187250873	38.0	34.0	40.0	26.0	41.0
33	35.03527266018089	38.0	34.0	40.0	25.0	41.0
34	34.74303743095569	37.0	33.0	40.0	24.0	41.0
35	34.55223728565992	37.0	33.0	40.0	23.0	41.0
36	34.367909144023464	37.0	33.0	40.0	23.0	41.0
37	34.182024262085875	37.0	33.0	40.0	23.0	41.0
38	34.05071465911231	37.0	33.0	40.0	22.0	41.0
39	33.962273551943234	37.0	33.0	40.0	22.0	41.0
40	33.74804638701717	36.0	33.0	40.0	21.0	41.0
41	33.59839607701444	36.0	32.0	40.0	20.0	41.0
42	33.48085545762399	36.0	32.0	40.0	20.0	41.0
43	33.43964412147956	36.0	32.0	40.0	20.0	41.0
44	33.22705537767288	35.0	32.0	40.0	19.0	41.0
45	33.2070373310168	35.0	32.0	40.0	20.0	41.0
46	33.14101760846252	35.0	32.0	40.0	20.0	41.0
47	33.02519805196053	35.0	31.0	39.0	20.0	41.0
48	32.92841877467241	35.0	31.0	39.0	20.0	41.0
49	32.90576724234026	35.0	31.0	39.0	20.0	41.0
50	32.76619922816432	35.0	31.0	39.0	19.0	41.0
51	32.5707336667192	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	8.0
13	19.0
14	36.0
15	108.0
16	296.0
17	745.0
18	1740.0
19	3203.0
20	5386.0
21	8460.0
22	12914.0
23	18104.0
24	24772.0
25	33109.0
26	41679.0
27	47258.0
28	51362.0
29	55626.0
30	64222.0
31	74709.0
32	91201.0
33	115028.0
34	172569.0
35	208773.0
36	171607.0
37	218078.0
38	293469.0
39	366740.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.06779892296634	22.041328572142422	27.09496884597496	14.795903658916279
2	34.17837745378788	24.75563021075573	28.408828831599138	12.657163503857257
3	30.059357931111357	25.434686095810637	31.258384295757597	13.247571677320408
4	26.669459830334524	26.842815355225074	31.33521231247045	15.15251250196995
5	23.16785119869003	31.466622334802945	30.45796032426324	14.90756614224378
6	20.256218312647263	41.68052037407893	27.490400101476403	10.5728612117974
7	86.0008206519859	3.5147736576478232	8.671716142820351	1.812689547545924
8	86.50925972193929	3.4847919925891473	7.831845140856168	2.174103144615408
9	82.16946175223613	4.416193558554577	9.529508877263519	3.8848358119457713
10	42.85129478511218	26.89086289538321	16.148153629126803	14.109688690377808
11	34.22065928912703	23.853537644286764	24.762501008998345	17.16330205758786
12	30.794052867669386	21.19674891124274	29.5900295588467	18.419168662241166
13	24.835485222498548	25.900458950103587	30.936706014398887	18.327349812998975
14	20.29114887434223	29.75079662821582	31.505396699710563	18.452657797731387
15	19.083858332788793	22.50662095103379	38.560265068669544	19.84925564750787
16	22.489996502139075	19.936077552573618	35.38437071175704	22.189555233530264
17	22.264989871578535	20.413525959124996	30.299403441741397	27.02208072755507
18	24.669769256493147	21.867732809551082	31.920335256516207	21.542162677439567
19	27.355290418551732	24.65290456989764	26.46006865032538	21.53173636122525
20	28.39047467125873	23.73697431186313	26.229824837887598	21.64272617899054
21	25.91343178594629	25.14625671224136	28.881712721835495	20.05859877997686
22	24.77028471050396	21.881570501116627	28.4360717868688	24.912073001510613
23	22.80326646397011	26.023989175850154	27.32314661418594	23.849597745993798
24	23.1413770040629	25.212658412739902	30.940309579910746	20.705655003286452
25	24.04284495250981	24.1065559907595	28.849953297790965	23.000645758939726
26	21.368422772227753	25.970079835792724	28.13947432147264	24.522023070506883
27	21.772694775118293	24.86993530879193	29.40850595212928	23.948863963960502
28	20.74687979274213	25.17373990521181	31.757742380621078	22.321637921424976
29	22.403943357715857	24.555944633858527	31.0182907375874	22.021821270838217
30	24.164837656971315	24.59803427903705	30.00213331078302	21.234994753208614
31	27.298594321165133	24.23104716730922	25.766454360602552	22.70390415092309
32	27.72174900733782	24.68913241517687	26.092504968115655	21.496613609369657
33	26.986669690458527	23.836432719990466	25.799174735450244	23.37772285410076
34	22.45487375028348	26.557749299466867	27.503949507801	23.483427442448658
35	23.289027094968844	24.87920848404245	28.249166855653655	23.582597565335046
36	27.46402200192959	26.27047305686138	24.982126315061173	21.283378626147854
37	23.251309775944712	28.419255147813455	25.661230247656242	22.668204828585594
38	24.92355636360841	28.384949204140547	24.13716227384023	22.554332158410816
39	23.61416479921894	27.043125550144335	24.442888771866436	24.89982087877029
40	25.530204605645007	24.575259745002096	26.16952517498914	23.725010474363756
41	22.482549133414565	24.24219419662591	27.016891593217995	26.25836507674153
42	23.847339511606364	25.399275058714093	25.51583839113773	25.237547038541813
43	23.37829942458266	24.953201695885976	26.266485111028253	25.402013768503107
44	22.9440938041736	25.472451462374934	27.448935074319934	24.13451965913153
45	22.18989156631137	26.98479583639236	26.154390199839327	24.67092239745694
46	24.176513209229743	26.236935873831	27.325356801033212	22.26119411590604
47	22.574416030196918	26.735333007891327	27.563624552677403	23.12662640923435
48	23.14651809085982	26.04109410014645	27.738517598853008	23.073870210140722
49	23.116440330720827	24.040154290260958	29.067464512086836	23.77594086693138
50	22.414898196871913	26.348358119457714	28.084796220772677	23.15194746289769
51	22.49840482166675	27.279038972320773	26.440849634262126	23.78170657175035
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	699.0
1	1534.5
2	2370.0
3	13902.0
4	25434.0
5	18136.5
6	10839.0
7	10663.0
8	10487.0
9	10503.5
10	10520.0
11	10232.0
12	9944.0
13	9648.0
14	9352.0
15	8788.5
16	8225.0
17	7885.0
18	7545.0
19	7332.5
20	7120.0
21	7360.0
22	7600.0
23	8102.0
24	8604.0
25	9280.5
26	12074.0
27	14191.0
28	15745.5
29	17300.0
30	21137.0
31	24974.0
32	29768.0
33	34562.0
34	40592.5
35	46623.0
36	50373.0
37	54123.0
38	60312.0
39	66501.0
40	81202.0
41	95903.0
42	105494.5
43	115086.0
44	122207.0
45	129328.0
46	143226.5
47	157125.0
48	156101.5
49	155078.0
50	151794.5
51	148511.0
52	144045.0
53	139579.0
54	131070.5
55	122562.0
56	121650.0
57	120738.0
58	116483.0
59	112228.0
60	107150.0
61	102072.0
62	93213.0
63	84354.0
64	75137.0
65	65920.0
66	57332.5
67	48745.0
68	41653.5
69	34562.0
70	28571.5
71	22581.0
72	19883.0
73	17185.0
74	13867.0
75	8387.0
76	6225.0
77	4430.5
78	2636.0
79	2128.5
80	1621.0
81	1180.0
82	739.0
83	608.5
84	478.0
85	351.5
86	225.0
87	172.5
88	120.0
89	95.0
90	70.0
91	66.0
92	62.0
93	38.0
94	14.0
95	9.0
96	4.0
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2081272.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.453666265591565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.66178938788015	18.214536898920016
2	6.515528722825644	3.0562607241805746
3	2.7147405294335782	1.9101185512503152
4	1.626716398454999	1.5260985407251448
5	1.1711702442002025	1.3734118023831465
6	0.886577340068144	1.2476093431556476
7	0.6813233981182304	1.1185672118882626
8	0.5883896649508881	1.1039915868705092
9	0.5292705736935516	1.117200185964607
>10	5.720936200615602	30.97271392263749
>50	1.560792789492297	25.73253956149861
>100	0.3355159592735983	10.107004133292797
>500	0.00434927459591726	0.7190482168483256
>1k	0.0026924080831868752	1.1509240322910375
>5k	0.0	0.0
>10k+	2.071083140912981E-4	0.6499752880934571
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13381	0.642924134855992	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACGAGATCGTATGCCG	3529	0.1695597692180551	No Hit
GAATCTGTCTCTTATACACATCTGACGCACACGAGATCGTATGCCGTCTTC	3299	0.15850883498168428	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACGAGATCGTATGC	2472	0.1187735192709074	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2161	0.10383073428172772	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.029981665058675656	0.0
2	0.0	0.0	0.0	0.13472530260340793	0.0
3	0.0	0.0	0.0	0.19377572945775468	0.0
4	0.0	0.0	0.0	0.3338823565588736	0.0
5	0.0	0.0	0.0	0.6915482454960236	0.0
6	0.0	0.0	0.0	0.9814190552700464	0.0
7	0.0	0.0	0.0	1.2121913906495643	0.0
8	0.0	0.0	0.0	1.8069238427269478	0.0
9	0.0	0.0	0.0	2.1099596785042993	0.0
10	0.0	0.0	0.0	2.7104097878605007	0.0
11	0.0	0.0	0.0	2.9798603930673164	0.0
12	0.0	0.0	0.0	3.347376027736884	0.0
13	0.0	0.0	0.0	3.469224589577912	0.0
14	0.0	0.0	0.0	3.5264011623660916	0.0
15	0.0	0.0	0.0	3.604382320042743	0.0
16	0.0	0.0	0.0	3.7239245999561805	0.0
17	0.0	0.0	0.0	3.856343620631998	0.0
18	0.0	0.0	0.0	4.060017143362328	0.0
19	0.0	0.0	0.0	4.162406451439312	0.0
20	0.0	0.0	0.0	4.270369274174639	0.0
21	0.0	0.0	0.0	4.38313685092578	0.0
22	0.0	0.0	0.0	4.505994411110128	0.0
23	0.0	0.0	0.0	4.645236182488401	0.0
24	0.0	0.0	0.0	4.752718529822147	0.0
25	0.0	0.0	0.0	4.8405974807713745	0.0
26	0.0	0.0	0.0	4.9305905234875596	0.0
27	0.0	0.0	0.0	5.031922785681064	0.0
28	0.0	0.0	0.0	5.130035862683974	0.0
29	0.0	0.0	0.0	5.248809381954882	0.0
30	0.0	0.0	0.0	5.407942834958622	0.0
31	0.0	0.0	0.0	5.534788340976095	0.0
32	0.0	0.0	0.0	5.658462709343133	0.0
33	0.0	0.0	0.0	5.784010931776336	0.0
34	4.8047540158134065E-5	0.0	0.0	5.918351854058479	0.0
35	4.8047540158134065E-5	0.0	0.0	6.084067820063884	0.0
36	4.8047540158134065E-5	0.0	0.0	6.217880219404288	0.0
37	4.8047540158134065E-5	0.0	0.0	6.356497372760504	0.0
38	4.8047540158134065E-5	0.0	0.0	6.532831845140856	0.0
39	4.8047540158134065E-5	0.0	0.0	6.815639666511633	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	9920	0.0	43.59375	1
CGGTCTA	405	0.0	41.666668	31
TACGGGA	670	0.0	39.626865	4
GTACGAG	285	0.0	39.473686	1
TAGGGAC	1800	0.0	38.5	5
ACGGGAT	745	0.0	37.75168	5
CACGACG	465	0.0	37.741936	26
GCGCGAC	1445	0.0	37.525948	9
TCACGAC	485	0.0	37.113403	25
GGGCGAT	3850	0.0	36.993507	7
TATGGGA	1560	0.0	36.92308	4
GTTTTTT	12325	0.0	36.876266	2
ATGGGCG	790	0.0	36.740505	5
TAGGGCG	980	0.0	36.734695	5
CGTAAGG	325	0.0	36.692307	2
CATATGC	1290	0.0	36.45349	33
CTCACGA	500	0.0	36.449997	24
CGGGATA	315	0.0	36.428574	6
GGCGATT	1090	0.0	36.330273	8
ACCCGCT	1400	0.0	36.321426	34
>>END_MODULE