FastQCFastQC Report
Thu 26 May 2016
SRR1546337_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546337_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1034850
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44890.4337826738174615No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC16990.16417838334058077No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC253.8905346E-545.00000424
GATCGAC253.8905346E-545.00000445
TGTTACG600.045.0000041
AACCGAT207.0329616E-445.016
ACACCGT207.0329616E-445.040
CTAAGCG207.0329616E-445.01
TAACGCG207.0329616E-445.01
CACTCGC351.2117744E-745.022
ACTCGAC207.0329616E-445.038
CACGTAC207.0329616E-445.025
GTTGCGT207.0329616E-445.026
GCGTAAG650.044.9999961
CGTTTTT33550.043.4575271
TACGCGG1150.043.043482
GCGATAC850.042.352949
TCGTAAA800.042.187515
TCGTTAG650.041.538461
CTATGCG603.6379788E-1241.2500041
GCGATAT1100.040.9090929
CGGTCTA1550.040.64516431
Produced by FastQC (version 0.11.5)