##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546337_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1034850
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.06710247862009	31.0	31.0	33.0	30.0	34.0
2	31.401673672512924	31.0	31.0	34.0	30.0	34.0
3	31.499616369522155	31.0	31.0	34.0	30.0	34.0
4	35.363338648113256	37.0	35.0	37.0	33.0	37.0
5	35.2564197709813	37.0	35.0	37.0	33.0	37.0
6	35.293571048944294	37.0	35.0	37.0	32.0	37.0
7	35.72164564912789	37.0	35.0	37.0	35.0	37.0
8	35.6164642218679	37.0	35.0	37.0	35.0	37.0
9	37.36553316905832	39.0	37.0	39.0	35.0	39.0
10	36.81603324153259	39.0	37.0	39.0	32.0	39.0
11	36.535112335121035	39.0	35.0	39.0	32.0	39.0
12	35.88246315891192	37.0	35.0	39.0	31.0	39.0
13	35.59795332656907	37.0	35.0	39.0	30.0	39.0
14	36.50843213992366	38.0	35.0	40.0	31.0	41.0
15	36.76760496690341	38.0	35.0	41.0	31.0	41.0
16	36.856663284533994	38.0	35.0	41.0	32.0	41.0
17	36.78168430207276	38.0	35.0	40.0	31.0	41.0
18	36.74064550417935	38.0	35.0	40.0	31.0	41.0
19	36.62428371261535	38.0	35.0	40.0	31.0	41.0
20	36.46963714548002	38.0	35.0	40.0	31.0	41.0
21	36.32037783253612	38.0	35.0	40.0	31.0	41.0
22	36.19673285983476	38.0	35.0	40.0	30.0	41.0
23	36.13897376431367	38.0	35.0	40.0	30.0	41.0
24	36.13612117698217	38.0	35.0	40.0	30.0	41.0
25	36.047070589940574	38.0	35.0	40.0	30.0	41.0
26	35.91567860076339	38.0	34.0	40.0	30.0	41.0
27	35.80184954341209	37.0	34.0	40.0	30.0	41.0
28	35.735704691501184	37.0	34.0	40.0	29.0	41.0
29	35.66118278011306	37.0	34.0	40.0	29.0	41.0
30	35.51342899937189	37.0	34.0	40.0	29.0	41.0
31	35.33212542880611	37.0	34.0	40.0	28.0	41.0
32	35.15049524085616	37.0	34.0	40.0	27.0	41.0
33	34.98597671160071	37.0	34.0	40.0	26.0	41.0
34	34.77281345122481	37.0	34.0	40.0	25.0	41.0
35	34.58680485094458	37.0	33.0	40.0	24.0	41.0
36	34.37088950089385	36.0	33.0	40.0	23.0	41.0
37	34.17266946900517	36.0	33.0	40.0	23.0	41.0
38	34.064788133545925	36.0	33.0	40.0	23.0	41.0
39	33.97974489056385	36.0	33.0	40.0	23.0	41.0
40	33.75553365222013	36.0	33.0	40.0	22.0	41.0
41	33.654043581195346	35.0	32.0	40.0	22.0	41.0
42	33.51278929313427	35.0	32.0	40.0	21.0	41.0
43	33.45856114412717	35.0	32.0	40.0	22.0	41.0
44	33.278701261052326	35.0	32.0	40.0	21.0	41.0
45	33.23362226409625	35.0	32.0	39.0	21.0	41.0
46	33.13435860269604	35.0	32.0	39.0	20.0	41.0
47	33.04945354399188	35.0	32.0	39.0	20.0	41.0
48	32.95751171667391	35.0	32.0	39.0	20.0	41.0
49	32.92135381939411	35.0	31.0	39.0	20.0	41.0
50	32.75603420785621	35.0	31.0	39.0	20.0	41.0
51	32.5462820698652	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	5.0
13	6.0
14	24.0
15	58.0
16	153.0
17	337.0
18	713.0
19	1459.0
20	2575.0
21	4055.0
22	5992.0
23	8450.0
24	11581.0
25	15585.0
26	20098.0
27	22983.0
28	25097.0
29	28273.0
30	32852.0
31	38900.0
32	47232.0
33	60137.0
34	96820.0
35	124888.0
36	79828.0
37	98689.0
38	135498.0
39	172537.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.096487413634826	22.39029811083732	28.172585398850075	14.34062907667778
2	32.072184374547035	24.7481277479828	30.014494854326713	13.165193023143452
3	29.988114219452093	24.879741025269364	31.876600473498574	13.255544281779969
4	26.261583804416098	27.651930231434505	30.703870126105233	15.382615838044162
5	23.27738319563222	31.78315697927236	30.365463593757546	14.573996231337874
6	19.86374836932889	41.25680050248828	27.84741750012079	11.032033628062038
7	87.33323670097116	3.859496545393052	6.980045417210223	1.827221336425569
8	88.15896023578297	2.830555152920713	6.528482388751993	2.48200222254433
9	84.00492825047108	4.057592887858143	8.724839348697879	3.2126395129728946
10	47.6135671836498	24.12871430642122	14.662511475093009	13.595207034835965
11	39.62294052278108	22.666859931391024	21.958641348987776	15.75155819684012
12	36.42498912885925	20.47900661931681	26.586751703145385	16.509252548678553
13	23.633956612069383	31.716867178818188	26.89452577668261	17.754650432429823
14	18.50780306324588	35.058220998212306	28.430207276416873	18.003768662124944
15	17.095714354737403	22.454075469874862	42.746195100739236	17.7040150746485
16	19.36908730733923	18.680871623906846	39.90665313813596	22.043387930617964
17	19.23621780934435	19.49915446683094	29.420882253466687	31.84374547035802
18	23.091462530801564	21.991109822679615	32.383050683673964	22.534376962844856
19	27.815335555877663	24.478040295695028	25.14799246267575	22.55863168575156
20	29.364642218679037	23.00816543460405	24.992124462482483	22.63506788423443
21	24.668599313910228	26.554959655988792	27.220756631395854	21.55568439870513
22	24.237329081509397	23.223848866985552	25.64980431946659	26.88901773203846
23	21.54747064791999	27.719959414407885	25.180267671643236	25.552302266028892
24	22.047446489829444	23.649224525293523	34.08919167029038	20.214137314586655
25	21.27622360728608	23.900178769870028	31.355655408996476	23.46794221384742
26	19.690389911581388	28.285258733149732	27.413828091027685	24.610523264241195
27	20.508092960332416	27.817558100207762	28.53176788906605	23.142581050393776
28	18.99183456539595	25.66729477702082	34.53167125670387	20.809199400879354
29	19.725177561965502	23.26018263516452	33.022080494757695	23.992559308112288
30	21.46330386046287	25.865004590037206	30.801951973715997	21.86973957578393
31	27.389959897569693	24.301879499444365	24.504614195294003	23.803546407691936
32	28.022515340387493	24.092573802966612	25.487655215731746	22.397255640914143
33	26.09489297965889	24.667246460839735	25.43151181330628	23.8063487461951
34	20.915978161086148	24.601826351645165	28.837222785911003	25.64497270135768
35	21.650480746001836	23.711359134174035	30.325264531091463	24.312895588732665
36	28.37358071218051	24.05720635840943	26.112769966661837	21.456442962748223
37	22.82920229985022	28.81818621056192	26.903609218727354	21.44900227086051
38	22.886988452432718	28.957433444460552	25.28076532830845	22.87481277479828
39	22.408851524375514	28.337826738174616	25.780741170217908	23.472580567231965
40	23.880369135623518	24.16659419239503	27.446779726530412	24.50625694545103
41	20.45813402908634	23.307629124993962	27.32347683239117	28.91076001352853
42	23.777745566990387	24.688022418708027	24.36758950572547	27.16664250857612
43	22.90167657148379	24.83799584480843	25.93013480214524	26.330192781562545
44	22.183118326327484	26.69179108083297	27.202589747306373	23.92250084553317
45	20.646857032420158	30.094506450210172	25.549403295163554	23.709233222206116
46	23.26308160602986	27.504469246750734	27.900565299318743	21.331883847900663
47	23.108083297096197	25.378074117021793	27.432768034014593	24.08107455186742
48	23.535681499734263	23.946272406628978	28.38672271343673	24.13132338020003
49	22.34816640092767	23.187804995893124	30.352998019036576	24.11103058414263
50	21.029134657196696	27.24578441319998	28.86669565637532	22.858385273228006
51	20.65381456249698	28.93211576556989	26.234816640092767	24.179253031840364
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	368.0
1	741.5
2	1115.0
3	4735.0
4	8355.0
5	6210.5
6	4066.0
7	4145.0
8	4224.0
9	4342.0
10	4460.0
11	4418.5
12	4377.0
13	4079.5
14	3782.0
15	3667.5
16	3553.0
17	3303.0
18	3053.0
19	2984.0
20	2915.0
21	2930.5
22	2946.0
23	3205.5
24	3465.0
25	3857.5
26	5174.5
27	6099.0
28	7290.5
29	8482.0
30	9516.0
31	10550.0
32	12980.0
33	15410.0
34	19066.0
35	22722.0
36	22711.5
37	22701.0
38	27208.0
39	31715.0
40	42932.5
41	54150.0
42	64353.5
43	74557.0
44	78573.5
45	82590.0
46	86668.5
47	90747.0
48	88420.5
49	86094.0
50	84025.5
51	81957.0
52	76184.5
53	70412.0
54	65038.5
55	59665.0
56	54825.0
57	49985.0
58	49117.5
59	48250.0
60	46000.0
61	43750.0
62	39884.5
63	36019.0
64	32443.0
65	28867.0
66	25176.5
67	21486.0
68	16937.0
69	12388.0
70	11106.0
71	9824.0
72	7894.0
73	5964.0
74	5057.0
75	3435.0
76	2720.0
77	1986.0
78	1252.0
79	934.0
80	616.0
81	518.0
82	420.0
83	333.0
84	246.0
85	158.5
86	71.0
87	49.0
88	27.0
89	25.0
90	23.0
91	14.5
92	6.0
93	4.5
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1034850.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.439217746467925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.24626214930105	18.574703941111466
2	6.227034833286538	2.919136507444875
3	2.4483446976642456	1.7216185346088733
4	1.4663480444603343	1.3748020442485283
5	1.0253100967870825	1.2016233308122266
6	0.7648880738004344	1.0757026868090886
7	0.6228599291648279	1.0219544653670791
8	0.5348293633223973	1.002878552329478
9	0.41941880760554245	0.8847763882587222
>10	5.06995230529416	27.543536183398835
>50	1.7716673692532134	29.66346048798032
>100	0.3993597315278101	11.966731737472255
>500	0.0028969099696317833	0.4488276165984479
>1k	8.276885627519381E-4	0.6002475235598003
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4489	0.4337826738174615	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1699	0.16417838334058077	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04677006329419723	0.0
2	0.0	0.0	0.0	0.19200850364787167	0.0
3	0.0	0.0	0.0	0.2715369377204426	0.0
4	0.0	0.0	0.0	0.42798473208677584	0.0
5	0.0	0.0	0.0	0.7577909842006088	0.0
6	0.0	0.0	0.0	1.1901241725853988	0.0
7	0.0	0.0	0.0	1.4947093781707494	0.0
8	0.0	0.0	0.0	2.4606464705029714	0.0
9	0.0	0.0	0.0	2.9092138957336813	0.0
10	0.0	0.0	0.0	3.550272986423153	0.0
11	0.0	0.0	0.0	3.899695608059139	0.0
12	0.0	0.0	0.0	4.268348069768566	0.0
13	0.0	0.0	0.0	4.408754892013335	0.0
14	0.0	0.0	0.0	4.456491278929313	0.0
15	0.0	0.0	0.0	4.521911388123883	0.0
16	0.0	0.0	0.0	4.661835048557762	0.0
17	0.0	0.0	0.0	4.820698651978548	0.0
18	0.0	0.0	0.0	5.105570855679567	0.0
19	0.0	0.0	0.0	5.204715659274291	0.0
20	0.0	0.0	0.0	5.342416775378074	0.0
21	0.0	0.0	0.0	5.482726965260666	0.0
22	0.0	0.0	0.0	5.62951152340919	0.0
23	0.0	0.0	0.0	5.793110112576702	0.0
24	0.0	0.0	0.0	5.927718993090786	0.0
25	0.0	0.0	0.0	6.025800840701551	0.0
26	0.0	0.0	0.0	6.121853408706576	0.0
27	0.0	0.0	0.0	6.223897183166643	0.0
28	0.0	0.0	0.0	6.325651060540175	0.0
29	0.0	0.0	0.0	6.440836836256462	0.0
30	0.0	0.0	0.0	6.608977146446345	0.0
31	0.0	0.0	0.0	6.726965260665797	0.0
32	0.0	0.0	0.0	6.845339904333962	0.0
33	0.0	0.0	0.0	6.960235782963714	0.0
34	0.0	0.0	0.0	7.088853457022757	0.0
35	0.0	0.0	0.0	7.254094796347297	0.0
36	0.0	0.0	0.0	7.376334734502585	0.0
37	0.0	0.0	0.0	7.500024158090545	0.0
38	0.0	0.0	0.0	7.64371648064937	0.0
39	0.0	0.0	0.0	7.8236459390249795	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	25	3.8905346E-5	45.000004	24
GATCGAC	25	3.8905346E-5	45.000004	45
TGTTACG	60	0.0	45.000004	1
AACCGAT	20	7.0329616E-4	45.0	16
ACACCGT	20	7.0329616E-4	45.0	40
CTAAGCG	20	7.0329616E-4	45.0	1
TAACGCG	20	7.0329616E-4	45.0	1
CACTCGC	35	1.2117744E-7	45.0	22
ACTCGAC	20	7.0329616E-4	45.0	38
CACGTAC	20	7.0329616E-4	45.0	25
GTTGCGT	20	7.0329616E-4	45.0	26
GCGTAAG	65	0.0	44.999996	1
CGTTTTT	3355	0.0	43.457527	1
TACGCGG	115	0.0	43.04348	2
GCGATAC	85	0.0	42.35294	9
TCGTAAA	80	0.0	42.1875	15
TCGTTAG	65	0.0	41.53846	1
CTATGCG	60	3.6379788E-12	41.250004	1
GCGATAT	110	0.0	40.909092	9
CGGTCTA	155	0.0	40.645164	31
>>END_MODULE