##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546336_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1995421
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11190821385562	31.0	31.0	33.0	30.0	34.0
2	31.42806254920641	31.0	31.0	34.0	30.0	34.0
3	31.48617860591825	31.0	31.0	34.0	30.0	34.0
4	35.35892375593922	37.0	35.0	37.0	33.0	37.0
5	35.26929354757718	37.0	35.0	37.0	33.0	37.0
6	35.297170872713075	37.0	35.0	37.0	32.0	37.0
7	35.7587947606044	37.0	35.0	37.0	35.0	37.0
8	35.66745513853969	37.0	35.0	37.0	35.0	37.0
9	37.41795039743493	39.0	37.0	39.0	35.0	39.0
10	36.91728061396567	39.0	37.0	39.0	32.0	39.0
11	36.58401660601948	39.0	35.0	39.0	32.0	39.0
12	35.66347953639858	37.0	35.0	39.0	31.0	39.0
13	35.253150087124475	37.0	35.0	39.0	30.0	39.0
14	36.115083483635786	38.0	35.0	40.0	30.0	41.0
15	36.39887221794298	38.0	35.0	40.0	31.0	41.0
16	36.532732190349805	38.0	35.0	40.0	31.0	41.0
17	36.4071075727879	38.0	35.0	40.0	31.0	41.0
18	36.34602973507846	38.0	35.0	40.0	31.0	41.0
19	36.21934719540388	37.0	35.0	40.0	31.0	41.0
20	36.02494060150715	36.0	34.0	40.0	31.0	41.0
21	35.864983379447246	36.0	34.0	40.0	30.0	41.0
22	35.73965093080608	36.0	34.0	40.0	30.0	41.0
23	35.7175683727895	36.0	34.0	40.0	30.0	41.0
24	35.71712385506618	36.0	34.0	40.0	30.0	41.0
25	35.671074926043175	36.0	34.0	40.0	30.0	41.0
26	35.54253312960022	36.0	34.0	40.0	30.0	41.0
27	35.417009242661074	36.0	34.0	40.0	30.0	41.0
28	35.36937317989537	36.0	34.0	40.0	29.0	41.0
29	35.28881273676081	36.0	34.0	40.0	29.0	41.0
30	35.10678247848449	36.0	34.0	40.0	29.0	41.0
31	34.84722622444086	35.0	34.0	40.0	27.0	41.0
32	34.587082124524095	35.0	33.0	40.0	26.0	41.0
33	34.33909235193977	35.0	33.0	40.0	24.0	41.0
34	34.05934186319579	35.0	33.0	40.0	23.0	41.0
35	33.82367931378892	35.0	33.0	40.0	23.0	41.0
36	33.60692705950273	35.0	33.0	40.0	22.0	41.0
37	33.41868658293162	35.0	32.0	40.0	21.0	41.0
38	33.36382898646451	35.0	32.0	40.0	22.0	41.0
39	33.331597191770555	35.0	32.0	39.0	21.0	41.0
40	33.07101057871998	35.0	32.0	39.0	20.0	41.0
41	32.99962263602518	35.0	32.0	39.0	20.0	41.0
42	32.872433937499906	35.0	32.0	39.0	19.0	41.0
43	32.811136096092	35.0	31.0	39.0	19.0	41.0
44	32.56669595037839	35.0	31.0	39.0	18.0	41.0
45	32.53121070691348	35.0	31.0	39.0	18.0	41.0
46	32.476753026053146	35.0	31.0	39.0	18.0	41.0
47	32.43378214421919	35.0	31.0	38.0	18.0	40.0
48	32.38816319964559	35.0	31.0	38.0	18.0	40.0
49	32.41669903243476	35.0	31.0	38.0	18.0	40.0
50	32.232932799644786	35.0	31.0	38.0	18.0	40.0
51	32.02288589726178	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	9.0
14	40.0
15	96.0
16	314.0
17	759.0
18	1704.0
19	3200.0
20	5530.0
21	8721.0
22	12882.0
23	18373.0
24	25225.0
25	35125.0
26	44431.0
27	49665.0
28	53684.0
29	58585.0
30	67573.0
31	79294.0
32	96936.0
33	123826.0
34	216030.0
35	281457.0
36	138950.0
37	167249.0
38	223125.0
39	282583.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.746612870166246	23.631704788112383	26.383655378990202	16.238026962731173
2	30.6233621877288	26.340807278263583	30.10908474953406	12.926745784473553
3	28.766711385717603	25.73236424794567	32.28742205278986	13.213502313546865
4	25.190974736659584	28.215148582680044	31.547778639194433	15.046098041465935
5	22.9011321420392	31.786324790608095	31.329579071283703	13.982963996069001
6	19.723156166042152	40.856941968637194	28.820133696097216	10.599768169223438
7	84.96537823346551	4.48627131818298	8.614823638720852	1.9335268096306493
8	85.74255758559221	3.7723367650235216	8.159230558363372	2.3258750910208925
9	82.77000191939446	4.439213579490243	9.830707404602839	2.960077096512465
10	52.803694057544746	20.47973836097746	14.205623775634315	12.510943805843478
11	45.04082095958697	19.741949192676632	20.155947040749798	15.061282806986595
12	41.39352046510486	18.245122207293598	25.683502378696026	14.67785494890552
13	24.618463973266795	34.288102610927716	24.93082913330069	16.162604282504795
14	17.45235717174471	38.08695007219028	28.776233185879068	15.684459570185942
15	16.35228856466881	20.9598375480663	47.15085187536865	15.537022011896237
16	19.53998679977809	16.78698379940875	44.28148245407861	19.391546946734547
17	17.921431116541324	17.247438009322344	30.054409570712142	34.77672130342419
18	22.93049937832668	21.88946593225189	33.56424533970526	21.615789349716174
19	28.691038131802767	25.708659977017383	24.998133226020975	20.60216866515888
20	31.735708905539234	22.558848483603207	25.095205472930278	20.610237137927285
21	25.021486693785423	27.1595317479369	27.657120978480233	20.161860579797448
22	23.4100974180386	23.13356429545444	26.59669312891866	26.859645157588297
23	21.455021271200415	28.211540321566225	24.521391726357493	25.812046680875866
24	22.91250818749527	21.90304702616641	35.782674433114614	19.401770353223704
25	20.77576611652378	21.89412660285724	32.99799891852396	24.332108362095017
26	17.075694803252045	30.188115690874255	28.221162351203077	24.51502715467062
27	18.48672535770647	30.019980745917778	28.580334676241254	22.9129592201345
28	15.966555428653903	27.643389540352636	36.50808526120553	19.88196976978793
29	19.026761771074877	24.758534665115782	34.65489237609507	21.559811187714274
30	21.47241108517952	26.828924823383137	31.492602312995604	20.206061778441743
31	29.721296909273782	24.357867337268676	24.89680122640786	21.024034527049682
32	30.55685993081159	24.90221361807859	26.433770116682144	18.10715633442767
33	28.533176708073132	26.375085758844875	24.982447313123394	20.109290219958595
34	21.65788572937741	27.633065904388097	28.76285255091532	21.94619581531917
35	21.164255563111745	26.419487416439942	30.47427084309527	21.94198617735305
36	30.254968750955314	27.057047109356873	24.938897606069098	17.74908653361872
37	24.123230135394987	30.866919812911664	26.3811496421056	18.62870040958775
38	24.533419263403562	30.748799376171743	22.14495086500543	22.572830495419264
39	23.52095121781318	28.957598421586223	24.30309192897138	23.218358431629216
40	25.717881088752698	23.23755237616523	24.798175422630113	26.24639111245196
41	20.250613780249882	23.235046639280633	25.901150684492148	30.613188895977338
42	23.814924269114137	25.18496096813655	22.89396573454925	28.10614902820006
43	23.495192242639522	25.979429904766967	23.696653488161147	26.828724364432365
44	21.88094642684426	27.672405973476273	26.875280955748188	23.57136664393128
45	20.50093689502115	32.056994488882296	23.99764260273897	23.44442601335758
46	24.204065207292093	29.796318671598627	26.442239507352085	19.557376613757196
47	23.260053893388914	25.950363356905637	26.720125727853922	24.06945702185153
48	24.00536027234353	22.315591546846505	30.06568538669283	23.613362794117133
49	22.930900296228216	20.783333441915264	31.50332686686168	24.78243939499484
50	20.563580317136083	27.554987143064047	27.207591781383478	24.673840758416393
51	20.013019808852366	29.01112096144122	25.421302071091766	25.55455715861465
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1233.0
1	1929.5
2	2626.0
3	14440.0
4	26254.0
5	18627.5
6	11001.0
7	11335.5
8	11670.0
9	11795.5
10	11921.0
11	11970.5
12	12020.0
13	11735.0
14	11450.0
15	10644.5
16	9839.0
17	9488.5
18	9138.0
19	8507.5
20	7877.0
21	8036.0
22	8195.0
23	7640.5
24	7086.0
25	7350.5
26	9617.5
27	11620.0
28	13719.0
29	15818.0
30	18157.5
31	20497.0
32	20534.5
33	20572.0
34	26913.0
35	33254.0
36	32229.5
37	31205.0
38	41652.0
39	52099.0
40	75454.0
41	98809.0
42	125575.0
43	152341.0
44	160111.5
45	167882.0
46	168113.5
47	168345.0
48	162462.0
49	156579.0
50	152174.5
51	147770.0
52	140524.0
53	133278.0
54	123621.0
55	113964.0
56	109216.0
57	104468.0
58	104458.5
59	104449.0
60	99205.5
61	93962.0
62	84801.5
63	75641.0
64	65962.0
65	56283.0
66	46044.5
67	35806.0
68	28822.0
69	21838.0
70	19104.0
71	16370.0
72	14030.5
73	11691.0
74	8866.0
75	4656.5
76	3272.0
77	2467.5
78	1663.0
79	1406.0
80	1149.0
81	825.0
82	501.0
83	346.0
84	191.0
85	135.5
86	80.0
87	53.0
88	26.0
89	20.0
90	14.0
91	15.5
92	17.0
93	9.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1995421.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.998346865140253
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.70192284508995	16.53901739470176
2	7.348204708917501	2.9390389320997823
3	2.3319571656667364	1.399058648209582
4	1.1582832222733048	0.9265499858837557
5	0.6340611553515689	0.6340087459216128
6	0.4348323196108461	0.5217556533450737
7	0.31992082757913404	0.4478521375517175
8	0.24761152687752985	0.3961456961843073
9	0.198473585060444	0.35722292378460097
>10	2.3088155471396075	10.753254829102588
>50	0.9364922274622973	13.718373447696381
>100	1.3650715981559096	48.02766423260172
>500	0.01032428251621575	1.3474341152360034
>1k	0.0035253647616346464	1.0506229377795993
>5k	2.51811768688189E-4	0.3109114709222665
>10k+	2.51811768688189E-4	0.6310888489792427
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12532	0.6280378927554636	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6174	0.3094083905100728	No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTG	2532	0.12689051583600652	No Hit
GGGGAGGGAGTGTTCTGCTGGTCTCCAATTACCAAGAATTCTCCAAAAATT	2026	0.10153245856388202	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11446206088840401	0.0
2	0.0	0.0	0.0	0.3698467641665593	0.0
3	0.0	0.0	0.0	0.50766229281941	0.0
4	0.0	0.0	0.0	0.72556117230399	0.0
5	0.0	0.0	0.0	1.1540922943078178	0.0
6	0.0	0.0	0.0	1.5436341503873117	0.0
7	0.0	0.0	0.0	1.800923213697761	0.0
8	0.0	0.0	0.0	2.4576768511507097	0.0
9	0.0	0.0	0.0	2.7744521081014986	0.0
10	0.0	0.0	0.0	3.398931854480834	0.0
11	0.0	0.0	0.0	3.786168432626498	0.0
12	0.0	0.0	0.0	4.238403825558616	0.0
13	0.0	0.0	0.0	4.399422477762838	0.0
14	0.0	0.0	0.0	4.461063605123932	0.0
15	0.0	0.0	0.0	4.5676075374570075	0.0
16	0.0	0.0	0.0	4.788212612776952	0.0
17	0.0	0.0	0.0	5.04520098766125	0.0
18	0.0	0.0	0.0	5.533719450682337	0.0
19	0.0	0.0	0.0	5.693334890231184	0.0
20	0.0	0.0	0.0	5.883219631345966	0.0
21	0.0	0.0	0.0	6.052156412105515	0.0
22	0.0	0.0	0.0	6.208965426343614	0.0
23	0.0	0.0	0.0	6.393137087361514	0.0
24	0.0	0.0	0.0	6.527695158064389	0.0
25	5.011473769194571E-5	0.0	0.0	6.634840467249768	0.0
26	5.011473769194571E-5	0.0	0.0	6.746445988089731	0.0
27	5.011473769194571E-5	0.0	0.0	6.85975541001122	0.0
28	5.011473769194571E-5	0.0	0.0	6.9696069150319655	0.0
29	5.011473769194571E-5	0.0	0.0	7.084620238034981	0.0
30	5.011473769194571E-5	0.0	0.0	7.241930399649998	0.0
31	5.011473769194571E-5	0.0	0.0	7.3769395029921006	0.0
32	5.011473769194571E-5	0.0	0.0	7.498016709255841	0.0
33	5.011473769194571E-5	0.0	0.0	7.628415256730284	0.0
34	5.011473769194571E-5	0.0	0.0	7.755756805205518	0.0
35	5.011473769194571E-5	0.0	0.0	7.905900559330587	0.0
36	5.011473769194571E-5	0.0	0.0	8.039406220541931	0.0
37	5.011473769194571E-5	0.0	0.0	8.169253505901763	0.0
38	5.011473769194571E-5	0.0	0.0	8.319146686338371	0.0
39	5.011473769194571E-5	0.0	0.0	8.475655012150318	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	20	7.034535E-4	45.000004	10
GTCGACT	40	6.8193913E-9	45.000004	33
CGCATTG	20	7.034535E-4	45.000004	28
CATTGCG	35	1.2125383E-7	45.000004	1
TAACGTT	30	2.166309E-6	45.000004	33
TCCTACG	30	2.166309E-6	45.000004	27
TACGCTA	20	7.034535E-4	45.000004	36
ACGGATC	30	2.166309E-6	45.000004	15
TCGCTAA	35	1.2125383E-7	45.000004	11
ACTACGT	20	7.034535E-4	45.000004	32
ACGCTAA	30	2.166309E-6	45.000004	25
ATGCGTA	30	2.166309E-6	45.000004	20
TTACGGC	35	1.2125383E-7	45.000004	31
TCGAGTA	20	7.034535E-4	45.000004	1
TAGGCCG	20	7.034535E-4	45.000004	28
CCGCGAA	20	7.034535E-4	45.000004	38
TGCACTA	20	7.034535E-4	45.000004	22
TGCGTAC	35	1.2125383E-7	45.000004	17
GTCGAAC	25	3.8918413E-5	45.0	30
CTATACG	50	2.1827873E-11	45.0	1
>>END_MODULE