FastQCFastQC Report
Thu 26 May 2016
SRR1546335_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546335_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1637955
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88060.5376216074312176No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC46070.281265358327915No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAC207.034165E-445.00000431
CCTAGAC207.034165E-445.00000419
TAGCGTC207.034165E-445.00000412
CCAATCG207.034165E-445.00000417
AATGCGT207.034165E-445.00000416
TACGATA207.034165E-445.00000422
TCGCTAA406.8175723E-945.00000412
ATCGGCA207.034165E-445.00000441
ATCGCTA406.8175723E-945.00000411
TCGAACG406.8175723E-945.0000041
AGCGTGC253.8915347E-545.043
AACCACG351.2123564E-745.01
TAGCCCG253.8915347E-545.039
CGTAGGT253.8915347E-545.034
TATAGCG253.8915347E-545.01
CCGTTTA253.8915347E-545.028
CAATACG253.8915347E-545.01
TACGTAG900.045.01
CTAACCG302.166069E-644.99999626
TATTACG302.166069E-644.9999961