FastQCFastQC Report
Thu 26 May 2016
SRR1546334_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546334_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1900314
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110170.5797462945597411No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC60610.3189472897636917No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTAG551.8189894E-1245.00000443
CGTATAT207.0344517E-445.023
TCCTCGA207.0344517E-445.044
CGTCTAC207.0344517E-445.017
ATATCGG800.045.02
ACGTATA207.0344517E-445.022
ACGTACT207.0344517E-445.016
CACGTAT253.8917715E-545.039
CGCGTAG207.0344517E-445.016
CCACGTA253.8917715E-545.038
TATCGAT253.8917715E-545.031
TACGTTG253.8917715E-545.013
CGTAGAT302.1662545E-644.99999625
TAACGGG4600.044.510873
CGTTTTT83350.042.9754031
AGTACGG1350.041.6666642
TATGCGT603.6379788E-1241.24999620
CGTAGGT603.6379788E-1241.2499963
ATTCGAT1150.041.0869614
TGCCGAT556.184564E-1140.90909229
Produced by FastQC (version 0.11.5)