##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546334_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1900314
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.153947189780215	31.0	31.0	33.0	30.0	34.0
2	31.491786620526923	31.0	31.0	34.0	30.0	34.0
3	31.543102350453662	31.0	31.0	34.0	30.0	34.0
4	35.403059178641	37.0	35.0	37.0	33.0	37.0
5	35.29064986102297	37.0	35.0	37.0	33.0	37.0
6	35.337504222986304	37.0	35.0	37.0	33.0	37.0
7	35.78397464840021	37.0	35.0	37.0	35.0	37.0
8	35.70390945917359	37.0	35.0	37.0	35.0	37.0
9	37.45278569752157	39.0	37.0	39.0	35.0	39.0
10	36.94663671372205	39.0	37.0	39.0	32.0	39.0
11	36.63463774934037	39.0	35.0	39.0	32.0	39.0
12	35.76754946814053	37.0	35.0	39.0	31.0	39.0
13	35.35095305302176	37.0	35.0	39.0	30.0	39.0
14	36.131960297087744	38.0	35.0	40.0	30.0	41.0
15	36.49883229824124	38.0	35.0	40.0	31.0	41.0
16	36.64801869585763	38.0	35.0	40.0	31.0	41.0
17	36.601605313648164	38.0	35.0	40.0	31.0	41.0
18	36.50954579085351	38.0	35.0	40.0	31.0	41.0
19	36.40311022283686	37.0	35.0	40.0	31.0	41.0
20	36.22031148536505	37.0	35.0	40.0	31.0	41.0
21	36.044647884507505	37.0	34.0	40.0	30.0	41.0
22	35.95216632619662	37.0	34.0	40.0	30.0	41.0
23	35.90163730836062	37.0	34.0	40.0	30.0	41.0
24	35.87290784575602	37.0	34.0	40.0	30.0	41.0
25	35.79329679200385	36.0	34.0	40.0	30.0	41.0
26	35.67620772146077	36.0	34.0	40.0	30.0	41.0
27	35.553106486612215	36.0	34.0	40.0	30.0	41.0
28	35.50894010147797	36.0	34.0	40.0	29.0	41.0
29	35.4242630428445	36.0	34.0	40.0	29.0	41.0
30	35.27941382318922	36.0	34.0	40.0	29.0	41.0
31	35.01321360575147	36.0	34.0	40.0	27.0	41.0
32	34.80474753119748	36.0	34.0	40.0	27.0	41.0
33	34.56943799814136	36.0	33.0	40.0	25.0	41.0
34	34.336079195332985	36.0	33.0	40.0	24.0	41.0
35	34.11545986610634	35.0	33.0	40.0	23.0	41.0
36	33.89349812715162	35.0	33.0	40.0	23.0	41.0
37	33.67590934971799	35.0	33.0	40.0	22.0	41.0
38	33.59207794080347	35.0	33.0	40.0	23.0	41.0
39	33.547131684553186	35.0	33.0	40.0	22.0	41.0
40	33.283186357623	35.0	32.0	40.0	21.0	41.0
41	33.1994007306161	35.0	32.0	39.0	20.0	41.0
42	33.114059571207704	35.0	32.0	39.0	20.0	41.0
43	33.03973395975613	35.0	32.0	39.0	20.0	41.0
44	32.861586558852906	35.0	31.0	39.0	19.0	41.0
45	32.83574188265729	35.0	31.0	39.0	20.0	41.0
46	32.807310265566635	35.0	32.0	39.0	20.0	41.0
47	32.730186169233086	35.0	31.0	39.0	20.0	41.0
48	32.652030664405984	35.0	31.0	39.0	19.0	41.0
49	32.67585251700508	35.0	31.0	39.0	20.0	40.0
50	32.50708409241841	35.0	31.0	38.0	18.0	40.0
51	32.278539230885	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	9.0
13	15.0
14	31.0
15	92.0
16	287.0
17	762.0
18	1560.0
19	2999.0
20	5089.0
21	8154.0
22	11827.0
23	16704.0
24	23387.0
25	31518.0
26	40206.0
27	46248.0
28	49117.0
29	53222.0
30	60758.0
31	72644.0
32	88153.0
33	114049.0
34	193547.0
35	260450.0
36	136058.0
37	165918.0
38	223305.0
39	294147.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.36386197228458	23.521270695264047	28.558806597225512	15.556060735225863
2	32.74821950477658	24.82089802001143	29.65236271479345	12.778519760418542
3	29.45386920266861	24.452590466628145	32.39959290938235	13.693947421320898
4	26.95712392794033	26.91539398225767	30.562949070522027	15.564533019279972
5	22.309049978056258	31.493953104592187	31.403020763936908	14.793976153414645
6	20.828084200821547	40.04401377877551	28.297323494959254	10.830578525443691
7	85.81066076448418	4.204199937484016	8.117342712835878	1.8677965851959202
8	87.43639209099128	3.1949456773985774	7.291847557824655	2.0768146737854902
9	83.4055319278814	4.324758960887516	9.213845711813942	3.055863399417149
10	51.45839056071786	21.527758044196908	14.787871899065102	12.22597949602013
11	45.00924584042426	20.750360203629505	20.15414294690246	14.086251009043767
12	39.4385348947595	20.348268759794433	26.236927160458745	13.976269184987322
13	24.491110416489065	34.56655058058826	26.407898905128313	14.534440097794366
14	17.318295818480525	38.61072433292603	29.45081707549384	14.620162773099604
15	15.495754912082951	22.16938884836927	47.112319332489264	15.222536907058517
16	18.499890018175943	16.637145229683096	44.541902022507855	20.321062729633102
17	19.477886286161127	16.616411814047574	29.87627307908062	34.029428820710685
18	24.234679110925878	21.22801810648135	33.29444502329615	21.242857759296623
19	29.034201716137435	23.339300768188835	26.30386346677444	21.322634048899285
20	30.590049854918718	22.533433948284337	24.889886618737744	21.986629578059205
21	24.951192276644807	25.08932734274441	28.74346029129923	21.216020089311556
22	23.50722038568363	22.298472778709204	26.69706164349681	27.49724519211036
23	20.60070072630102	28.100829652362712	25.578246542413517	25.720223078922743
24	21.850125821311636	24.1170669689325	34.966063503189474	19.06674370656639
25	20.76467362762154	22.62236661941132	33.6119714952371	23.000988257730036
26	18.579350570484667	29.80581103964924	27.814192812345752	23.800645577520346
27	19.59928727568181	28.81913199608065	29.92652793169971	21.655052796537834
28	17.80200535279959	26.23945305881028	36.34720367265621	19.611337915733927
29	18.610240202408654	24.724440276712166	35.00695148275496	21.658368038124227
30	21.691257339576513	26.001281893413402	32.684703685811925	19.62275708119816
31	29.691408893477604	23.841586179968154	26.20682687176961	20.26017805478463
32	29.67746382966184	24.813215079192176	26.68669493567905	18.82262615546694
33	28.632952238419545	24.741700582114323	25.92324215892742	20.70210502053871
34	21.496973658037565	25.937713451566424	28.797398745680976	23.76791414471503
35	21.892960847523092	25.09537897421163	30.132757007526124	22.87890317073915
36	31.36434294542902	22.821596851888685	26.450155079634207	19.363905123048085
37	22.974729439450535	28.424828738829476	28.004266663298804	20.596175158421186
38	22.909003459428284	30.032510416699555	23.94783177937962	23.11065434449254
39	22.783918868144948	27.887706978951897	26.04295921621374	23.28541493668941
40	24.598829456605593	24.64382202099232	25.66386397195411	25.093484550447982
41	20.22223695662927	22.365882690965808	27.77567286248483	29.636207489920086
42	24.386127766253367	24.78953478214653	24.82047703695284	26.003860414647264
43	23.776123314357523	24.71170553918984	26.51088188583571	25.001289260616932
44	22.359410076439996	27.49971846757957	27.55960330766389	22.581268148316543
45	20.266755915075088	31.94756235022212	24.340819464572697	23.44486227013009
46	24.461167996446903	27.678057415774443	26.803570357319895	21.057204230458755
47	23.380188747754318	26.450891799986735	27.200294267157954	22.96862518510099
48	23.394396925981706	24.839368651707034	28.643792552178223	23.12244187013304
49	23.43186441819615	22.110609088813742	30.659985665526857	23.79754082746325
50	21.831181583675118	28.096356707365207	27.65642941113942	22.416032297820255
51	21.184235868388065	30.697032174682708	25.190100162394213	22.928631794535008
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	721.0
1	1374.0
2	2027.0
3	10437.0
4	18847.0
5	13819.5
6	8792.0
7	9092.0
8	9392.0
9	9811.5
10	10231.0
11	10141.5
12	10052.0
13	9696.5
14	9341.0
15	8820.5
16	8300.0
17	7853.5
18	7407.0
19	7187.0
20	6967.0
21	6663.5
22	6360.0
23	6070.5
24	5781.0
25	7084.0
26	8604.5
27	8822.0
28	10926.0
29	13030.0
30	15453.5
31	17877.0
32	21937.5
33	25998.0
34	30916.5
35	35835.0
36	37695.5
37	39556.0
38	48552.5
39	57549.0
40	80865.5
41	104182.0
42	126431.0
43	148680.0
44	159061.5
45	169443.0
46	168000.5
47	166558.0
48	161957.5
49	157357.0
50	152622.0
51	147887.0
52	136317.0
53	124747.0
54	115031.0
55	105315.0
56	101258.5
57	97202.0
58	93415.5
59	89629.0
60	80311.0
61	70993.0
62	66920.5
63	62848.0
64	56981.5
65	51115.0
66	42838.5
67	34562.0
68	28087.0
69	21612.0
70	17460.0
71	13308.0
72	12099.0
73	10890.0
74	8498.5
75	4808.5
76	3510.0
77	2604.0
78	1698.0
79	1176.0
80	654.0
81	480.0
82	306.0
83	269.0
84	232.0
85	163.0
86	94.0
87	68.5
88	43.0
89	40.0
90	37.0
91	26.0
92	15.0
93	14.0
94	13.0
95	8.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1900314.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.68907817260586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.10108280098055	16.164946373240923
2	6.954312782309473	2.738480160152867
3	2.3198542303043213	1.3702737386853654
4	1.1125878477898665	0.8762331643610396
5	0.7553340208147826	0.7435915291125478
6	0.4875515461698436	0.5759664303427743
7	0.3932992889239871	0.5420590311398275
8	0.3245423257549983	0.5111951377687581
9	0.26298601379529124	0.46601569655257424
>10	2.8694760526680825	13.053092314459827
>50	1.0149814727690813	14.520852456719933
>100	1.3927081029966237	45.796845660801864
>500	0.008328308486111281	1.106948630023409
>1k	0.0024178960120968237	0.6301466540578617
>5k	2.6865511245520267E-4	0.3206009292575086
>10k+	2.6865511245520267E-4	0.5827520933228794
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11017	0.5797462945597411	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6061	0.3189472897636917	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03594142862705848	0.0
2	0.0	0.0	0.0	0.14697571032997706	0.0
3	0.0	0.0	0.0	0.22559429652152224	0.0
4	0.0	0.0	0.0	0.349836921687679	0.0
5	0.0	0.0	0.0	0.5946911931396601	0.0
6	0.0	0.0	0.0	0.8719611601030146	0.0
7	0.0	0.0	0.0	1.0578251804701748	0.0
8	0.0	0.0	0.0	1.5872113766461753	0.0
9	0.0	0.0	0.0	1.8695857631949246	0.0
10	0.0	0.0	0.0	2.3047243771292534	0.0
11	0.0	0.0	0.0	2.615883480309044	0.0
12	0.0	0.0	0.0	2.9156234180246003	0.0
13	0.0	0.0	0.0	3.0436022678357366	0.0
14	0.0	0.0	0.0	3.091120730574	0.0
15	0.0	0.0	0.0	3.154478680891684	0.0
16	0.0	0.0	0.0	3.3020858658095453	0.0
17	0.0	0.0	0.0	3.46542729254218	0.0
18	0.0	0.0	0.0	3.7411185730358247	0.0
19	0.0	0.0	0.0	3.838207790922974	0.0
20	5.262288232365809E-5	0.0	0.0	3.9692387679088825	0.0
21	5.262288232365809E-5	0.0	0.0	4.094533850721512	0.0
22	5.262288232365809E-5	0.0	0.0	4.218513361476051	0.0
23	5.262288232365809E-5	0.0	0.0	4.355859084340798	0.0
24	5.262288232365809E-5	0.0	0.0	4.452948302227948	0.0
25	5.262288232365809E-5	0.0	0.0	4.528725252774016	0.0
26	5.262288232365809E-5	0.0	0.0	4.6150794026671385	0.0
27	5.262288232365809E-5	0.0	0.0	4.720588281726073	0.0
28	5.262288232365809E-5	0.0	0.0	4.812520457145503	0.0
29	5.262288232365809E-5	0.0	0.0	4.914240488677135	0.0
30	5.262288232365809E-5	0.0	0.0	5.047639495367608	0.0
31	5.262288232365809E-5	0.0	0.0	5.1580423024826425	0.0
32	5.262288232365809E-5	0.0	0.0	5.254763160193526	0.0
33	5.262288232365809E-5	0.0	0.0	5.367376128366154	0.0
34	5.262288232365809E-5	0.0	0.0	5.482935977948907	0.0
35	5.262288232365809E-5	0.0	0.0	5.62596497210461	0.0
36	5.262288232365809E-5	0.0	0.0	5.739419906394417	0.0
37	5.262288232365809E-5	0.0	0.0	5.860715650150449	0.0
38	5.262288232365809E-5	0.0	0.0	5.992956953429801	0.0
39	5.262288232365809E-5	0.0	0.0	6.223076817831158	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTAG	55	1.8189894E-12	45.000004	43
CGTATAT	20	7.0344517E-4	45.0	23
TCCTCGA	20	7.0344517E-4	45.0	44
CGTCTAC	20	7.0344517E-4	45.0	17
ATATCGG	80	0.0	45.0	2
ACGTATA	20	7.0344517E-4	45.0	22
ACGTACT	20	7.0344517E-4	45.0	16
CACGTAT	25	3.8917715E-5	45.0	39
CGCGTAG	20	7.0344517E-4	45.0	16
CCACGTA	25	3.8917715E-5	45.0	38
TATCGAT	25	3.8917715E-5	45.0	31
TACGTTG	25	3.8917715E-5	45.0	13
CGTAGAT	30	2.1662545E-6	44.999996	25
TAACGGG	460	0.0	44.51087	3
CGTTTTT	8335	0.0	42.975403	1
AGTACGG	135	0.0	41.666664	2
TATGCGT	60	3.6379788E-12	41.249996	20
CGTAGGT	60	3.6379788E-12	41.249996	3
ATTCGAT	115	0.0	41.08696	14
TGCCGAT	55	6.184564E-11	40.909092	29
>>END_MODULE