FastQCFastQC Report
Thu 26 May 2016
SRR1546333_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546333_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1753087
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86260.49204631601283905No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC63740.3635872036014185No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGT351.212411E-745.023
CTTAACG207.0343027E-445.01
GTACACG453.8562575E-1045.01
ACGTTGC207.0343027E-445.030
ACGTTAG950.045.01
CTATACG207.0343027E-445.01
GCACGTT253.8916474E-545.015
TTCGTAC453.8562575E-1045.033
AGTATCC207.0343027E-445.045
TTATACG750.045.01
TACGCAT253.8916474E-545.030
TATCTAG950.045.01
TCGCTAA453.8562575E-1045.016
AGTCTAA207.0343027E-445.029
TATTACG253.8916474E-545.01
CGAATAT1950.045.014
CGTACCG207.0343027E-445.045
TATAGCG1000.045.01
CCGCGTA207.0343027E-445.014
CCAACGA207.0343027E-445.035