##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546333_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1753087
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16798481763883	31.0	31.0	33.0	30.0	34.0
2	31.498220567490375	31.0	31.0	34.0	30.0	34.0
3	31.54858657898895	31.0	31.0	34.0	30.0	34.0
4	35.405647295313926	37.0	35.0	37.0	33.0	37.0
5	35.31684394442489	37.0	35.0	37.0	33.0	37.0
6	35.358745458725096	37.0	35.0	37.0	33.0	37.0
7	35.7938898639942	37.0	35.0	37.0	35.0	37.0
8	35.709427427161344	37.0	35.0	37.0	35.0	37.0
9	37.4859359518381	39.0	37.0	39.0	35.0	39.0
10	37.00199761905713	39.0	37.0	39.0	33.0	39.0
11	36.67903760623403	39.0	35.0	39.0	32.0	39.0
12	35.67213150288605	37.0	35.0	39.0	31.0	39.0
13	35.198830406020924	37.0	35.0	39.0	30.0	39.0
14	36.02824046952604	38.0	35.0	40.0	30.0	41.0
15	36.41180386369872	38.0	35.0	40.0	31.0	41.0
16	36.589833248435475	38.0	35.0	40.0	32.0	41.0
17	36.53613996339029	38.0	35.0	40.0	32.0	41.0
18	36.44358893768535	37.0	35.0	40.0	31.0	41.0
19	36.304562180884346	37.0	35.0	40.0	31.0	41.0
20	36.092346244082584	36.0	35.0	40.0	31.0	41.0
21	35.92890541085525	36.0	34.0	40.0	31.0	41.0
22	35.79320535717851	36.0	34.0	40.0	30.0	41.0
23	35.75693162974798	36.0	34.0	40.0	30.0	41.0
24	35.735013151087195	36.0	34.0	40.0	30.0	41.0
25	35.65633080389051	35.0	34.0	40.0	30.0	41.0
26	35.521137285257375	35.0	34.0	40.0	30.0	41.0
27	35.38465347127667	35.0	34.0	40.0	30.0	41.0
28	35.36064781725037	36.0	34.0	40.0	29.0	41.0
29	35.319485570311116	36.0	34.0	40.0	29.0	41.0
30	35.16834133160533	36.0	34.0	40.0	29.0	41.0
31	34.865328417813835	35.0	34.0	40.0	27.0	41.0
32	34.584599623407165	35.0	34.0	40.0	26.0	41.0
33	34.30966232708359	35.0	33.0	40.0	25.0	41.0
34	34.0505998846606	35.0	33.0	40.0	23.0	41.0
35	33.78337013508172	35.0	33.0	40.0	23.0	41.0
36	33.537180984172494	35.0	33.0	40.0	22.0	41.0
37	33.33840248658509	35.0	32.0	39.0	21.0	41.0
38	33.28574908147742	35.0	32.0	39.0	22.0	41.0
39	33.27535142294706	35.0	32.0	39.0	21.0	41.0
40	32.980509238845535	35.0	32.0	39.0	19.0	41.0
41	32.907571615099535	35.0	32.0	39.0	19.0	41.0
42	32.783896064485106	35.0	32.0	39.0	18.0	41.0
43	32.693887981600454	35.0	31.0	39.0	18.0	41.0
44	32.52811355055397	35.0	31.0	39.0	18.0	41.0
45	32.45824765114338	35.0	31.0	38.0	18.0	41.0
46	32.376758255580015	35.0	31.0	38.0	18.0	40.0
47	32.29095589665544	35.0	31.0	38.0	18.0	40.0
48	32.213310577284524	35.0	31.0	38.0	18.0	40.0
49	32.2297569943762	35.0	31.0	38.0	18.0	40.0
50	32.04358768275619	35.0	31.0	38.0	18.0	40.0
51	31.827829993605565	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	3.0
12	2.0
13	11.0
14	17.0
15	77.0
16	211.0
17	623.0
18	1378.0
19	2609.0
20	4616.0
21	7567.0
22	11276.0
23	16047.0
24	22615.0
25	31106.0
26	39459.0
27	44241.0
28	47009.0
29	50542.0
30	57346.0
31	68373.0
32	84159.0
33	108312.0
34	190536.0
35	269441.0
36	117550.0
37	143215.0
38	189999.0
39	244697.0
40	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.49278957633021	23.247220474511533	29.83987674313939	15.420113206018868
2	31.543728291864582	25.969618164985537	29.99081049599934	12.495843047150542
3	27.529666240180894	25.428002147069712	33.25299885288066	13.789332759868733
4	25.132295202691026	28.31017513677302	31.371061447606426	15.186468212929535
5	22.617702373014005	31.6015691177905	31.21801713206475	14.562711377130741
6	20.024847597409597	41.708141124770194	27.48528738163023	10.781723896189979
7	84.94826554529239	4.750534343132999	8.34596343478675	1.9552366767878606
8	86.68337623860081	3.4871058880705865	7.4354552854478975	2.3940625878806925
9	83.08555137309214	4.486257670041476	9.407690548158762	3.020500408707611
10	54.603793194519156	19.819837806110023	13.903987651497046	11.67238134787378
11	48.20314108769274	18.812300815646914	19.59298083894296	13.391577257717385
12	42.372968369510474	18.739743093183627	24.62490452555977	14.262384011746137
13	22.04876312470516	38.56226188432177	25.305874722703436	14.08310026826963
14	15.490560365800443	42.21193814111906	28.283593455430335	14.013908037650157
15	12.82771476829159	21.399052072144737	50.34724460337679	15.425988556186887
16	15.227823833044225	15.132392174489913	49.10703233781324	20.53275165465262
17	15.734472961125146	16.33096360876557	30.072780187178388	37.8617832429309
18	21.302251399959044	21.023428957034078	35.782650832502895	21.891668810503987
19	29.794242955426625	23.108778971037946	25.371644419244454	21.725333654290974
20	31.52547477677948	21.36454152018696	24.719993930706234	22.38998977232733
21	22.418111594005317	27.604847905437662	28.168197014751694	21.808843485805326
22	22.323763737909186	23.234214845013394	25.25527826057691	29.186743156500505
23	18.781840262348645	29.466991655291498	24.52890244465905	27.22226563770081
24	20.73365440505805	22.4531925683095	38.4136668630821	18.399486163550353
25	18.409069258970035	22.58096717390523	36.01213174246344	22.997831824661297
26	17.142446438767728	32.105080922966174	27.538165533142394	23.214307105123705
27	18.353738291368312	31.655245860587637	29.00631856833118	20.984697279712872
28	16.638535337949573	27.375595164415685	37.974840951989265	18.011028545645484
29	16.541962834702442	22.732414306876954	37.59619459844264	23.12942825997797
30	19.698851226436567	27.825030931151733	32.41396462354692	20.06215321886478
31	30.136781574445536	24.90458260200435	24.13987440440777	20.818761419142348
32	31.568769832871958	24.283107455591193	26.06328151426598	18.08484119727087
33	29.186400903092657	25.297945852088343	24.74492138724433	20.770731857574667
34	21.409319674380107	26.554700365697766	27.052108651766854	24.98387130815527
35	21.399622494491148	23.529351367045674	31.73288034193397	23.338145796529208
36	31.763112726293674	22.776222743081203	25.659479535242692	19.80118499538243
37	22.07420396135503	30.476182870559192	28.346453998004662	19.10315917008112
38	22.743252331458734	30.850950352150235	23.083680387795926	23.3221169285951
39	21.86542938256915	29.381542387799353	26.061113909349622	22.69191432028188
40	25.250372628397795	24.265709574025703	25.85176890821733	24.632148889359172
41	18.808364901456688	22.812843857720694	27.782933761986712	30.595857478835903
42	24.346937716154418	24.62553199014082	23.393590848600212	27.63393944510455
43	23.564318256880576	24.10228357177938	25.60078307579715	26.73261509554289
44	21.667778039538256	27.31786842295904	27.208290290213778	23.806063247288925
45	18.519331898530993	34.652016699684616	23.554621076991616	23.27403032479278
46	24.30324336441945	29.952478114320623	25.619093633116897	20.12518488814303
47	23.95751038026065	26.629026397434924	25.84988651447418	23.563576707830244
48	24.300676463860608	23.025782519635364	29.086177696828507	23.58736331967552
49	21.659449873280675	22.335115142602735	31.90794296004705	24.097492024069542
50	20.34240171765577	29.731097201679095	27.179826215127946	22.74667486553719
51	19.39487315803494	31.685991625059113	23.992477270095552	24.926657946810398
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1281.0
1	1639.0
2	1997.0
3	8840.0
4	15683.0
5	12080.0
6	8477.0
7	9133.0
8	9789.0
9	10582.5
10	11376.0
11	11404.0
12	11432.0
13	10996.5
14	10561.0
15	10201.0
16	9841.0
17	9353.0
18	8865.0
19	8217.5
20	7570.0
21	7103.5
22	6637.0
23	6614.5
24	6592.0
25	6506.0
26	7931.0
27	9442.0
28	10531.0
29	11620.0
30	13486.5
31	15353.0
32	18930.5
33	22508.0
34	25766.0
35	29024.0
36	31067.5
37	33111.0
38	41527.5
39	49944.0
40	74333.0
41	98722.0
42	128914.5
43	159107.0
44	165019.0
45	170931.0
46	164049.5
47	157168.0
48	153217.0
49	149266.0
50	139384.5
51	129503.0
52	118690.0
53	107877.0
54	97987.0
55	88097.0
56	85098.5
57	82100.0
58	80357.5
59	78615.0
60	72235.5
61	65856.0
62	58457.0
63	51058.0
64	45924.5
65	40791.0
66	33850.0
67	26909.0
68	23772.0
69	20635.0
70	17547.0
71	14459.0
72	12592.0
73	10725.0
74	8619.0
75	4894.5
76	3276.0
77	2612.5
78	1949.0
79	1510.5
80	1072.0
81	833.5
82	595.0
83	384.5
84	174.0
85	125.5
86	77.0
87	64.0
88	51.0
89	37.0
90	23.0
91	16.0
92	9.0
93	7.5
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1753087.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.08538308762077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.97778854045167	16.666406705985604
2	6.86334770914588	2.757059360034788
3	2.283265001663139	1.3758075674688366
4	1.1179508042959438	0.8981788070959115
5	0.6880356158965986	0.6909729461605141
6	0.48038400421218114	0.5789218052260131
7	0.350587228453969	0.4929175152387632
8	0.27192461352422975	0.43693680268699026
9	0.24177659800713588	0.43705580333355115
>10	2.4155500861686234	11.032185866692231
>50	0.8685937869420353	12.785034363601447
>100	1.4276484975474901	49.2862695270241
>500	0.01200425163101636	1.557972402759789
>1k	5.716310300483981E-4	0.14317340994446787
>5k	5.716310300483981E-4	0.8611071167470001
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8626	0.49204631601283905	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6374	0.3635872036014185	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.052250686931110665	0.0
2	0.0	0.0	0.0	0.2433992152129358	0.0
3	0.0	0.0	0.0	0.3744252281831991	0.0
4	0.0	0.0	0.0	0.5999131817188764	0.0
5	0.0	0.0	0.0	1.0001785421944263	0.0
6	0.0	0.0	0.0	1.5104783732923694	0.0
7	0.0	0.0	0.0	1.8010515165533714	0.0
8	0.0	0.0	0.0	2.6532054598545307	0.0
9	0.0	0.0	0.0	3.0744053204433093	0.0
10	0.0	0.0	0.0	3.738605100602537	0.0
11	0.0	0.0	0.0	4.188782416388919	0.0
12	0.0	0.0	0.0	4.617454809715661	0.0
13	0.0	0.0	0.0	4.800560382913113	0.0
14	0.0	0.0	0.0	4.861310362805725	0.0
15	0.0	0.0	0.0	4.948185686163892	0.0
16	0.0	0.0	0.0	5.147091958356887	0.0
17	0.0	0.0	0.0	5.35552428373492	0.0
18	0.0	0.0	0.0	5.713122052699039	0.0
19	0.0	0.0	0.0	5.838557926674489	0.0
20	0.0	0.0	0.0	6.004265618306451	0.0
21	0.0	0.0	0.0	6.172483168262613	0.0
22	0.0	0.0	0.0	6.324329596876823	0.0
23	0.0	0.0	0.0	6.505609818565764	0.0
24	0.0	0.0	0.0	6.6344682266196715	0.0
25	0.0	0.0	0.0	6.732866081375311	0.0
26	0.0	0.0	0.0	6.832689991996975	0.0
27	0.0	0.0	0.0	6.941412491222626	0.0
28	0.0	0.0	0.0	7.04785330106264	0.0
29	0.0	0.0	0.0	7.162679319394873	0.0
30	0.0	0.0	0.0	7.329641940188935	0.0
31	0.0	0.0	0.0	7.467798232489318	0.0
32	0.0	0.0	0.0	7.583593968810447	0.0
33	0.0	0.0	0.0	7.703268577087161	0.0
34	0.0	0.0	0.0	7.822543889721389	0.0
35	0.0	0.0	0.0	7.985798765263789	0.0
36	0.0	0.0	0.0	8.11003675231178	0.0
37	0.0	0.0	0.0	8.24442825712586	0.0
38	0.0	0.0	0.0	8.363760611994728	0.0
39	0.0	0.0	0.0	8.487770430104153	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	35	1.212411E-7	45.0	23
CTTAACG	20	7.0343027E-4	45.0	1
GTACACG	45	3.8562575E-10	45.0	1
ACGTTGC	20	7.0343027E-4	45.0	30
ACGTTAG	95	0.0	45.0	1
CTATACG	20	7.0343027E-4	45.0	1
GCACGTT	25	3.8916474E-5	45.0	15
TTCGTAC	45	3.8562575E-10	45.0	33
AGTATCC	20	7.0343027E-4	45.0	45
TTATACG	75	0.0	45.0	1
TACGCAT	25	3.8916474E-5	45.0	30
TATCTAG	95	0.0	45.0	1
TCGCTAA	45	3.8562575E-10	45.0	16
AGTCTAA	20	7.0343027E-4	45.0	29
TATTACG	25	3.8916474E-5	45.0	1
CGAATAT	195	0.0	45.0	14
CGTACCG	20	7.0343027E-4	45.0	45
TATAGCG	100	0.0	45.0	1
CCGCGTA	20	7.0343027E-4	45.0	14
CCAACGA	20	7.0343027E-4	45.0	35
>>END_MODULE