FastQCFastQC Report
Thu 26 May 2016
SRR1546332_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546332_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1770606
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83550.471872342011718No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39550.22336985190381148No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGT253.8916627E-545.014
ACACGTA207.034321E-445.026
CGTGCAA207.034321E-445.029
CGTTGTA207.034321E-445.020
CCAATCG207.034321E-445.015
CGTTAGT207.034321E-445.044
TCGTAAT351.2124292E-745.021
ACCGTAG207.034321E-445.044
CGCGTAG207.034321E-445.015
CGATCTA207.034321E-445.010
CAATCGT207.034321E-445.016
CGGACTA253.8916627E-545.034
ACTAACG302.1661708E-644.9999961
CGTTTTT72700.043.607291
GTTACAC1150.043.04347633
AGGGTAC8900.042.471916
TATAGCG750.042.0000041
ATCGCCA1400.041.78571324
ATTCGGC1200.041.24999617
TAGGGTA8500.040.7647065
Produced by FastQC (version 0.11.5)