##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546332_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1770606
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18083243815959	31.0	31.0	33.0	30.0	34.0
2	31.51895622176814	31.0	31.0	34.0	30.0	34.0
3	31.607204539010937	31.0	31.0	34.0	30.0	34.0
4	35.48158540070462	37.0	35.0	37.0	33.0	37.0
5	35.40418873538212	37.0	35.0	37.0	33.0	37.0
6	35.440615811761624	37.0	35.0	37.0	33.0	37.0
7	35.795785171856416	37.0	35.0	37.0	35.0	37.0
8	35.69294580499558	37.0	35.0	37.0	35.0	37.0
9	37.4956094128225	39.0	37.0	39.0	35.0	39.0
10	37.00218569235618	39.0	37.0	39.0	33.0	39.0
11	36.711054859183804	39.0	35.0	39.0	32.0	39.0
12	36.03469659540293	37.0	35.0	39.0	32.0	39.0
13	35.73805126606371	37.0	35.0	39.0	31.0	39.0
14	36.70194893725651	38.0	35.0	41.0	31.0	41.0
15	36.98171755884709	38.0	35.0	41.0	32.0	41.0
16	37.059080902244766	38.0	35.0	41.0	32.0	41.0
17	36.97895805165011	38.0	35.0	41.0	32.0	41.0
18	36.90518443967771	38.0	35.0	40.0	32.0	41.0
19	36.76476810764224	38.0	35.0	40.0	31.0	41.0
20	36.590620951244944	38.0	35.0	40.0	31.0	41.0
21	36.42161723161448	38.0	35.0	40.0	31.0	41.0
22	36.3127827421798	38.0	35.0	40.0	31.0	41.0
23	36.26377353290342	38.0	35.0	40.0	31.0	41.0
24	36.24162744280772	38.0	35.0	40.0	31.0	41.0
25	36.161854190034376	38.0	35.0	40.0	31.0	41.0
26	36.035356821336876	37.0	35.0	40.0	30.0	41.0
27	35.925312011819685	37.0	35.0	40.0	30.0	41.0
28	35.881475607786264	37.0	35.0	40.0	30.0	41.0
29	35.786875228029274	37.0	35.0	40.0	30.0	41.0
30	35.637013542256156	37.0	34.0	40.0	30.0	41.0
31	35.40845055308748	37.0	34.0	40.0	29.0	41.0
32	35.19690264237216	37.0	34.0	40.0	28.0	41.0
33	34.97203386863029	37.0	34.0	40.0	27.0	41.0
34	34.722160096599694	36.0	34.0	40.0	25.0	41.0
35	34.509260671205226	36.0	33.0	40.0	24.0	41.0
36	34.27571972533698	36.0	33.0	40.0	23.0	41.0
37	34.06242947329897	36.0	33.0	40.0	23.0	41.0
38	33.96379714063998	35.0	33.0	40.0	23.0	41.0
39	33.91567519820897	35.0	33.0	40.0	23.0	41.0
40	33.67679144880341	35.0	33.0	40.0	22.0	41.0
41	33.57654384995872	35.0	33.0	40.0	22.0	41.0
42	33.4636192354482	35.0	32.0	40.0	22.0	41.0
43	33.40037026871026	35.0	32.0	39.0	22.0	41.0
44	33.182871852913635	35.0	32.0	39.0	21.0	41.0
45	33.1481628323862	35.0	32.0	39.0	21.0	41.0
46	33.05718945942802	35.0	32.0	39.0	21.0	41.0
47	32.970434981017796	35.0	32.0	39.0	21.0	41.0
48	32.875836860374356	35.0	32.0	39.0	20.0	41.0
49	32.87394937100631	35.0	32.0	39.0	20.0	40.0
50	32.70670154737982	35.0	31.0	38.0	20.0	40.0
51	32.48703212346507	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	1.0
12	5.0
13	9.0
14	20.0
15	78.0
16	221.0
17	519.0
18	1133.0
19	2183.0
20	3805.0
21	5993.0
22	9205.0
23	13137.0
24	18553.0
25	25226.0
26	32762.0
27	38252.0
28	41453.0
29	46031.0
30	53572.0
31	64007.0
32	79663.0
33	101840.0
34	172124.0
35	236205.0
36	139932.0
37	175843.0
38	231291.0
39	277493.0
40	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.03761537010492	23.134395794434223	29.124661274162634	14.703327561298222
2	31.330346785224943	25.596998993564917	29.989732328931453	13.08292189227869
3	28.58885601878679	25.41672173255936	32.630861976069205	13.36356027258464
4	25.52340836978978	27.5504544771677	31.52316212641322	15.402975026629301
5	22.611749875466366	32.091781006051036	30.508481277031706	14.787987841450892
6	20.179136408664604	41.7230033107309	27.423322862342047	10.674537418262448
7	87.28034356598813	4.096507071590179	6.975408419490277	1.6477409429314032
8	88.97851921884372	2.826659347138776	6.290671103565672	1.904150330451834
9	85.46672721090971	3.8084700944196506	8.129476574686858	2.5953261199837794
10	50.50897828201192	21.116612052596683	13.369490445644033	15.004919219747364
11	39.76243161945684	20.05296491709618	22.12722649759461	18.05737696585237
12	34.51846429979341	18.975028888414474	28.460933714219877	18.045573097572245
13	21.25904916169944	32.02073188501564	29.752073583846432	16.968145369438485
14	16.583983110867127	34.34727997081225	31.837800165593023	17.230936752727597
15	14.810466021237925	20.45503065052304	45.71288022292933	19.021623105309708
16	18.004965531575063	16.126456139875277	42.389950107477326	23.478628221072334
17	17.653955764297645	17.485821238604185	30.057844602356482	34.80237839474169
18	22.016981756528555	19.87760122805412	34.06178449638146	24.043632519035857
19	28.345097667126396	22.318573414977696	25.351094484035407	23.985234433860498
20	30.153348627532043	21.54273734529308	24.351719128931	23.952194898243878
21	23.82687057425537	24.81280420375849	28.099023724080908	23.261301497905237
22	23.69358287501567	21.90973034091153	24.995679445342443	29.401007338730356
23	21.237079282460357	26.644888812079024	24.18776396329844	27.930267942162175
24	21.736964632447876	22.326649745906206	34.804863419642764	21.131522202003154
25	22.184212636803444	21.31050047271951	32.360954385108826	24.14433250536822
26	20.174900570765036	27.459186289891708	27.018715626175442	25.347197513167806
27	19.740416557946826	27.668549637807622	27.8339167494067	24.75711705483885
28	19.19608314893319	24.612929132737605	34.04410693288061	22.146880785448598
29	18.905448191184263	21.98552360039444	34.22410180469286	24.884926403728443
30	21.576680526328275	25.633314243823868	30.04423344323921	22.745771786608653
31	29.133415339155068	23.847428507527933	23.668958537359526	23.350197615957473
32	29.734396020345578	23.19335865799619	25.87153776729549	21.200707554362744
33	28.42478789747691	24.056565944089197	24.430392758185615	23.088253400248277
34	22.174554926392435	25.450947302787856	26.937500494181087	25.436997276638618
35	22.14891398764039	23.93124162010069	29.251510499794986	24.668333892463938
36	31.9354503486377	22.580122285816266	24.421299826161214	21.063127539384823
37	23.693018096629064	28.539211998603868	25.81714960866506	21.950620296102013
38	23.878095973920793	29.4328608397351	22.395835098265792	24.29320808807832
39	22.863245691023298	27.68120067366766	24.09672168737709	25.358831947931954
40	25.205268704612998	24.1699169662816	24.92824490598134	25.69656942312406
41	20.187890473657042	22.59282979951497	26.636868958989186	30.58241076783881
42	23.867873485123173	24.802920581992833	23.11649231957872	28.212713613305272
43	23.968573471455535	24.40712388865733	25.063113984703545	26.561188655183592
44	22.285985702070366	25.807943720963333	27.031423139874143	24.87464743709216
45	20.28774329240949	30.354692122358106	24.395207064699882	24.962357520532517
46	23.83517281653852	27.723107229954035	26.556388038897417	21.885331914610028
47	23.827265919125995	25.625689735604645	26.4014693274506	24.145575017818757
48	23.71758595644655	24.42689113218864	27.76970144684927	24.08582146451554
49	23.186581317356882	22.755316541342342	29.67933012765121	24.378772013649563
50	22.201268944079033	27.25055715387839	27.237341339631744	23.310832562410834
51	22.08910395649851	29.41405371946102	23.735207042108748	24.761635281931724
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1006.0
1	1576.5
2	2147.0
3	9106.5
4	16066.0
5	11921.0
6	7776.0
7	8142.0
8	8508.0
9	8880.5
10	9253.0
11	9305.0
12	9357.0
13	9036.5
14	8716.0
15	8283.0
16	7850.0
17	7420.0
18	6990.0
19	6781.0
20	6572.0
21	6347.0
22	6122.0
23	5794.0
24	5466.0
25	6192.5
26	7835.5
27	8752.0
28	9711.0
29	10670.0
30	13623.5
31	16577.0
32	19376.0
33	22175.0
34	24182.5
35	26190.0
36	29205.0
37	32220.0
38	39498.0
39	46776.0
40	65518.5
41	84261.0
42	101108.5
43	117956.0
44	125582.5
45	133209.0
46	134597.0
47	135985.0
48	131988.0
49	127991.0
50	130005.0
51	132019.0
52	125753.5
53	119488.0
54	111554.5
55	103621.0
56	103694.0
57	103767.0
58	99120.0
59	94473.0
60	91681.5
61	88890.0
62	81864.5
63	74839.0
64	66682.0
65	58525.0
66	49582.5
67	40640.0
68	36292.0
69	31944.0
70	26367.5
71	20791.0
72	18260.0
73	15729.0
74	12357.5
75	7077.0
76	5168.0
77	4216.0
78	3264.0
79	2436.5
80	1609.0
81	1181.0
82	753.0
83	536.5
84	320.0
85	226.5
86	133.0
87	101.5
88	70.0
89	52.5
90	35.0
91	27.0
92	19.0
93	14.0
94	9.0
95	6.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1770606.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.21540508485492
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.27185186456536	15.616715555739452
2	6.709361331517329	2.5784619169153413
3	2.180052292198776	1.2567176370229798
4	1.1050128587460335	0.849330788191144
5	0.7492806235951023	0.7198865352306296
6	0.5345948928289852	0.6163474453202131
7	0.41178496904786144	0.5538830491676371
8	0.3613119654046451	0.5554204621804274
9	0.29427511115813515	0.508915392056485
>10	3.4365177989935365	15.60035611571979
>50	1.5871033903494485	22.442950789524257
>100	1.3520527238888769	37.11407780328415
>500	0.004730558404143142	0.5607936951537834
>1k	0.0017739594015536782	0.551476486562433
>5k	2.9565990025894637E-4	0.4746663279312998
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8355	0.471872342011718	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3955	0.22336985190381148	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04049461031985659	0.0
2	0.0	0.0	0.0	0.13746705930060105	0.0
3	0.0	0.0	0.0	0.20213418456731763	0.0
4	0.0	0.0	0.0	0.3203987787232168	0.0
5	0.0	0.0	0.0	0.5346192207639644	0.0
6	0.0	0.0	0.0	0.8317491299588954	0.0
7	0.0	0.0	0.0	1.0121393466417712	0.0
8	0.0	0.0	0.0	1.5814359603435209	0.0
9	0.0	0.0	0.0	1.8816156728261397	0.0
10	0.0	0.0	0.0	2.333833726983869	0.0
11	0.0	0.0	0.0	2.6276314436978074	0.0
12	0.0	0.0	0.0	2.910924282420821	0.0
13	0.0	0.0	0.0	3.021733801873483	0.0
14	0.0	0.0	0.0	3.063301491127896	0.0
15	0.0	0.0	0.0	3.117294304887705	0.0
16	0.0	0.0	0.0	3.240641904523084	0.0
17	0.0	0.0	0.0	3.38669359530014	0.0
18	0.0	0.0	0.0	3.6439501504004843	0.0
19	0.0	0.0	0.0	3.733298091162009	0.0
20	0.0	0.0	0.0	3.852692242091126	0.0
21	0.0	0.0	0.0	3.9756444968558786	0.0
22	0.0	0.0	0.0	4.092666578561238	0.0
23	0.0	0.0	0.0	4.234143564406763	0.0
24	0.0	0.0	0.0	4.338119265381457	0.0
25	0.0	0.0	0.0	4.41526799299223	0.0
26	0.0	0.0	0.0	4.494111055762829	0.0
27	0.0	0.0	0.0	4.571824561760211	0.0
28	0.0	0.0	0.0	4.656710753267525	0.0
29	0.0	0.0	0.0	4.749955664896651	0.0
30	0.0	0.0	0.0	4.874828166175874	0.0
31	0.0	0.0	0.0	4.97631884224949	0.0
32	0.0	0.0	0.0	5.07368663610086	0.0
33	0.0	0.0	0.0	5.180881573879225	0.0
34	0.0	0.0	0.0	5.285591486756512	0.0
35	0.0	0.0	0.0	5.418935663834868	0.0
36	0.0	0.0	0.0	5.525565823226624	0.0
37	0.0	0.0	0.0	5.634850441035442	0.0
38	0.0	0.0	0.0	5.740463999331302	0.0
39	0.0	0.0	0.0	5.859462805389793	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGT	25	3.8916627E-5	45.0	14
ACACGTA	20	7.034321E-4	45.0	26
CGTGCAA	20	7.034321E-4	45.0	29
CGTTGTA	20	7.034321E-4	45.0	20
CCAATCG	20	7.034321E-4	45.0	15
CGTTAGT	20	7.034321E-4	45.0	44
TCGTAAT	35	1.2124292E-7	45.0	21
ACCGTAG	20	7.034321E-4	45.0	44
CGCGTAG	20	7.034321E-4	45.0	15
CGATCTA	20	7.034321E-4	45.0	10
CAATCGT	20	7.034321E-4	45.0	16
CGGACTA	25	3.8916627E-5	45.0	34
ACTAACG	30	2.1661708E-6	44.999996	1
CGTTTTT	7270	0.0	43.60729	1
GTTACAC	115	0.0	43.043476	33
AGGGTAC	890	0.0	42.47191	6
TATAGCG	75	0.0	42.000004	1
ATCGCCA	140	0.0	41.785713	24
ATTCGGC	120	0.0	41.249996	17
TAGGGTA	850	0.0	40.764706	5
>>END_MODULE