##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546331_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	691471
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11138138837348	31.0	31.0	33.0	30.0	34.0
2	31.44726966134516	31.0	31.0	34.0	30.0	34.0
3	31.521180208569845	31.0	31.0	34.0	30.0	34.0
4	35.389769057559896	37.0	35.0	37.0	33.0	37.0
5	35.311869912114894	37.0	35.0	37.0	33.0	37.0
6	35.341415619744	37.0	35.0	37.0	33.0	37.0
7	35.73885383479568	37.0	35.0	37.0	35.0	37.0
8	35.63556389205043	37.0	35.0	37.0	35.0	37.0
9	37.34047993335946	39.0	37.0	39.0	35.0	39.0
10	36.85679948978338	39.0	37.0	39.0	32.0	39.0
11	36.613612139916206	39.0	35.0	39.0	32.0	39.0
12	36.14512683829112	38.0	35.0	39.0	32.0	39.0
13	35.93892585516963	38.0	35.0	39.0	31.0	39.0
14	36.862028920952575	39.0	35.0	41.0	31.0	41.0
15	37.06641204041818	39.0	35.0	41.0	31.0	41.0
16	37.12120392612272	39.0	35.0	41.0	32.0	41.0
17	37.0320042344509	39.0	35.0	41.0	32.0	41.0
18	36.93511513859583	38.0	35.0	40.0	31.0	41.0
19	36.84111842723701	38.0	35.0	40.0	31.0	41.0
20	36.693807838651225	38.0	35.0	40.0	31.0	41.0
21	36.54000384687138	38.0	35.0	40.0	31.0	41.0
22	36.43795618326727	38.0	35.0	40.0	30.0	41.0
23	36.34523067489454	38.0	35.0	40.0	30.0	41.0
24	36.352620717282434	38.0	35.0	40.0	30.0	41.0
25	36.29075839767684	38.0	35.0	40.0	30.0	41.0
26	36.16092504240959	38.0	35.0	40.0	30.0	41.0
27	36.044535490280865	38.0	35.0	40.0	30.0	41.0
28	35.96376854560784	38.0	35.0	40.0	30.0	41.0
29	35.85142543938936	38.0	34.0	40.0	30.0	41.0
30	35.67842324551572	38.0	34.0	40.0	29.0	41.0
31	35.49658915558281	38.0	34.0	40.0	28.0	41.0
32	35.322820479817665	38.0	34.0	40.0	27.0	41.0
33	35.12797499822842	38.0	34.0	40.0	26.0	41.0
34	34.883204067849555	37.0	34.0	40.0	25.0	41.0
35	34.670299983658026	37.0	33.0	40.0	24.0	41.0
36	34.46937326366543	37.0	33.0	40.0	23.0	41.0
37	34.27239898708695	37.0	33.0	40.0	23.0	41.0
38	34.15824669436607	37.0	33.0	40.0	23.0	41.0
39	34.072983537993636	37.0	33.0	40.0	23.0	41.0
40	33.88180415375337	36.0	33.0	40.0	22.0	41.0
41	33.753266586740445	36.0	33.0	40.0	21.0	41.0
42	33.642375457539075	36.0	32.0	40.0	21.0	41.0
43	33.603276203918895	36.0	32.0	40.0	21.0	41.0
44	33.41135492305534	35.0	32.0	40.0	21.0	41.0
45	33.36874142227223	35.0	32.0	40.0	21.0	41.0
46	33.27907171811977	35.0	32.0	40.0	20.0	41.0
47	33.18425790814076	35.0	32.0	39.0	20.0	41.0
48	33.0648226751375	35.0	32.0	39.0	20.0	41.0
49	33.04676985730421	35.0	31.0	39.0	20.0	41.0
50	32.92489345178612	35.0	31.0	39.0	20.0	41.0
51	32.720825602230605	35.0	31.0	39.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	9.0
14	14.0
15	39.0
16	117.0
17	237.0
18	510.0
19	961.0
20	1590.0
21	2663.0
22	3754.0
23	5402.0
24	7634.0
25	10323.0
26	13336.0
27	15433.0
28	16622.0
29	17931.0
30	21109.0
31	25134.0
32	30419.0
33	38070.0
34	58640.0
35	73568.0
36	56476.0
37	70961.0
38	98325.0
39	122175.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.96998283369802	22.735299094249793	26.64536907549268	14.649348996559509
2	33.73952052942206	23.856531944217473	28.887111679303977	13.516835847056493
3	30.364252441534063	24.644417480993418	31.293430960951362	13.697899116521157
4	26.172030352682903	27.453935161416748	30.49672365146188	15.877310834438466
5	23.04319342387461	31.092988715361887	30.30409084401226	15.559727016751246
6	21.423024248305424	40.32157530829203	27.50455189010096	10.750848553301584
7	84.69595977271642	4.6959308488714635	8.205260958160213	2.402848420251898
8	85.17320321459613	3.880278420931608	8.164767575212844	2.7817507892594193
9	79.94680904911414	5.519971191850417	10.387998918248197	4.145220840787249
10	43.69308329633492	24.47015131509492	16.999556018979828	14.837209369590337
11	35.39425369972132	24.41736529803853	23.499177839706945	16.689203162533207
12	30.977582573961886	22.364495401831746	28.667435076814503	17.990486947391865
13	23.83006662607687	28.7708378225551	29.692785380731802	17.706310170636225
14	19.93243389816782	31.80523839756114	30.84467750635963	17.417650197911406
15	18.222022326315926	23.09106238728739	40.24290244999429	18.444012836402393
16	21.910246416697156	20.454075442064816	36.47846402813711	21.15721411310091
17	21.675674034052044	20.725381107812186	29.88064575376263	27.71829910437314
18	24.988755855270863	22.28003777454152	31.750427711357382	20.980778658830232
19	28.10689096144307	24.136659382678378	26.1263306776423	21.630118978236254
20	29.112717670010746	23.58001998637687	25.7945741759235	21.512688167688886
21	25.657909008476132	25.12064858829944	28.70214369076939	20.519298712455043
22	24.608985770914472	22.500726711604678	27.791621051352838	25.098666466128005
23	22.699867384170847	26.823395341236296	26.271528379353583	24.20520889523928
24	22.53688151780769	24.87089118704906	32.12556998051979	20.46665731462346
25	22.812814998749044	24.766186868285146	30.026566551597973	22.394431581367837
26	21.383109342257303	26.738214617822003	27.77455598282502	24.10412005709567
27	21.332058755898657	25.945412027402448	29.504635769251347	23.217893447447544
28	20.211982859729474	25.660946012197183	32.406998992004006	21.720072136069334
29	21.240659405817453	24.93336669216786	31.31020679102956	22.515767110985134
30	23.599688200951306	25.021005942403946	30.333882404323536	21.04542345232121
31	27.90485790438066	24.271155261753567	26.192277044156587	21.631709789709184
32	27.22211054404306	24.676812187351313	26.39647938959118	21.704597879014447
33	26.565828501846067	25.049929787366352	26.363072348659593	22.021169362127985
34	22.775358619522727	25.791971029876887	28.823623839611496	22.609046510988893
35	23.09525634480694	26.210499066482907	28.566057000221267	22.12818758848889
36	27.56861820669269	25.751333027704703	26.12488448539418	20.555164280208423
37	23.345736842181378	28.375593481143824	27.054496862485916	21.224172814188883
38	23.82066637646409	28.67018284208593	25.443872555754325	22.065278225695657
39	22.845065085882126	27.874054009495698	25.56650965839493	23.714371246227245
40	24.40030022951071	25.035034007210715	26.852897663097945	23.71176810018063
41	21.822751785685877	24.57919421060319	26.89411414216937	26.703939861541553
42	23.507276516296418	26.028423462444554	25.572728285061846	24.89157173619718
43	23.161058092096415	25.842443139336286	26.745011721388174	24.25148704717913
44	22.98476725705055	26.898452718913735	27.28675533753404	22.830024686501677
45	20.994517485187377	28.890437921474653	26.16176238772125	23.953282205616723
46	23.5936141935092	26.777551046970878	27.723360777241563	21.90547398227836
47	22.891777095496412	27.04697666279569	27.4870529638987	22.574193277809194
48	23.59086642823777	25.573306761961096	28.379642819438562	22.456183990362575
49	23.143993023568594	24.343464874159583	28.99181599806789	23.520726104203938
50	21.7076348827355	27.618656458477652	27.857422798642318	22.816285860144532
51	22.329352930202422	28.317890410443823	26.215849977800946	23.136906681552805
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	268.0
1	547.5
2	827.0
3	4018.5
4	7210.0
5	5241.0
6	3272.0
7	3329.0
8	3386.0
9	3365.5
10	3345.0
11	3260.0
12	3175.0
13	3106.0
14	3037.0
15	2967.0
16	2897.0
17	2762.0
18	2627.0
19	2546.0
20	2465.0
21	2583.0
22	2701.0
23	2978.0
24	3255.0
25	3707.0
26	4770.5
27	5382.0
28	5944.0
29	6506.0
30	7530.5
31	8555.0
32	10515.5
33	12476.0
34	14215.5
35	15955.0
36	17703.0
37	19451.0
38	22219.0
39	24987.0
40	29818.0
41	34649.0
42	39197.0
43	43745.0
44	45536.0
45	47327.0
46	48288.0
47	49249.0
48	50759.0
49	52269.0
50	52176.5
51	52084.0
52	49305.0
53	46526.0
54	44030.0
55	41534.0
56	39260.5
57	36987.0
58	34742.5
59	32498.0
60	31226.5
61	29955.0
62	27220.5
63	24486.0
64	22676.5
65	20867.0
66	17899.0
67	14931.0
68	12647.5
69	10364.0
70	8690.0
71	7016.0
72	5803.5
73	4591.0
74	3781.0
75	2379.5
76	1788.0
77	1336.0
78	884.0
79	620.5
80	357.0
81	283.0
82	209.0
83	157.0
84	105.0
85	78.0
86	51.0
87	45.5
88	40.0
89	37.0
90	34.0
91	24.5
92	15.0
93	8.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	691471.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.396651205766272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.85218533032605	20.971547476088773
2	5.627299655675616	3.1959293110509823
3	2.74751943110238	2.3406105289823893
4	1.8594351186636426	2.112069220177763
5	1.4213078543448128	2.0180191697922853
6	1.194624956559998	2.035400892788277
7	1.0484276670508563	2.0840284343002895
8	0.9060161252142561	2.058225911560727
9	0.8795092782251142	2.247760638539443
>10	10.342931433481363	57.36285922599128
>50	0.10027820878721334	1.7781302006495185
>100	0.019441693540316243	1.098056054945104
>500	5.116235142188484E-4	0.13613105629578756
>1k	5.116235142188484E-4	0.5612318788373825
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3863	0.5586640654488764	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	937	0.1355082136488732	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03731176000150404	0.0
2	0.0	0.0	0.0	0.1317481138037604	0.0
3	0.0	0.0	0.0	0.1930666651240616	0.0
4	0.0	0.0	0.0	0.28432139598045325	0.0
5	0.0	0.0	0.0	0.4901145528879736	0.0
6	0.0	0.0	0.0	0.7829684831323367	0.0
7	0.0	0.0	0.0	0.9821091556985037	0.0
8	0.0	0.0	0.0	1.5143079030067783	0.0
9	0.0	0.0	0.0	1.8138143175924948	0.0
10	0.0	0.0	0.0	2.2524444264473855	0.0
11	0.0	0.0	0.0	2.528088668939117	0.0
12	0.0	0.0	0.0	2.7813169315849833	0.0
13	0.0	0.0	0.0	2.885587392674458	0.0
14	0.0	0.0	0.0	2.9304193523661874	0.0
15	0.0	0.0	0.0	2.9859531346940074	0.0
16	0.0	0.0	0.0	3.099913083845888	0.0
17	0.0	0.0	0.0	3.226021047881979	0.0
18	0.0	0.0	0.0	3.4164845669594244	0.0
19	0.0	0.0	0.0	3.499351382776718	0.0
20	0.0	0.0	0.0	3.5987047902225835	0.0
21	0.0	0.0	0.0	3.716569458444389	0.0
22	0.0	0.0	0.0	3.843689757054164	0.0
23	0.0	0.0	0.0	3.9855612165947667	0.0
24	0.0	0.0	0.0	4.097351877374467	0.0
25	0.0	0.0	0.0	4.184123412261686	0.0
26	0.0	0.0	0.0	4.270461089474468	0.0
27	0.0	0.0	0.0	4.367645208548153	0.0
28	0.0	0.0	0.0	4.464540089172214	0.0
29	0.0	0.0	0.0	4.576909226851162	0.0
30	0.0	0.0	0.0	4.7235531208105614	0.0
31	0.0	0.0	0.0	4.84517788887748	0.0
32	0.0	0.0	0.0	4.958559361130113	0.0
33	0.0	0.0	0.0	5.081630321445151	0.0
34	0.0	0.0	0.0	5.209907573853423	0.0
35	0.0	0.0	0.0	5.3533698448669575	0.0
36	0.0	0.0	0.0	5.473114563011319	0.0
37	0.0	0.0	0.0	5.605875011388764	0.0
38	0.0	0.0	0.0	5.7716086430233515	0.0
39	0.0	0.0	0.0	6.024547667219594	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGG	35	1.2109922E-7	45.000004	2
CGGATAC	25	3.8891878E-5	45.0	14
TATTACG	20	7.031335E-4	45.0	1
TATAGCG	25	3.8891878E-5	45.0	1
CGTTTTT	2905	0.0	43.141136	1
CGGGATA	75	0.0	42.0	6
CGACGGT	70	0.0	41.785717	28
CGGTAGT	135	0.0	41.666668	12
TACGGGT	55	6.002665E-11	40.90909	4
GCGATAT	45	1.9263098E-8	40.0	9
TATACTA	445	0.0	39.4382	44
AATGCGG	40	3.4555524E-7	39.375	2
TAGGGTA	290	0.0	38.793102	5
ATACTAT	465	0.0	37.741936	45
GGGCGAT	1560	0.0	37.644234	7
TGATTAG	60	1.546141E-10	37.500004	1
TACGAAA	60	1.546141E-10	37.500004	20
CATACGA	60	1.546141E-10	37.500004	18
CCGCGAA	30	1.13945134E-4	37.500004	45
ACATACG	60	1.546141E-10	37.500004	17
>>END_MODULE