FastQCFastQC Report
Thu 26 May 2016
SRR1546330_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546330_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences221955
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7880.35502691987114504No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC6130.2761821089860557No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACAC207.020977E-445.026
ACGCCGG351.2060082E-745.027
AGTCACG207.020977E-445.01
GATCGAG207.020977E-445.01
CTCCGAC253.880605E-545.023
GTTTACG253.880605E-545.01
CGTTGCA207.020977E-445.033
CCGGATA207.020977E-445.020
TAAGTTA207.020977E-445.042
GTGATAG207.020977E-445.01
ATTCGCT207.020977E-445.039
TAAGTCA207.020977E-445.012
ACGGGTG207.020977E-445.05
TCTAGAG453.8198777E-1045.01
GACCCAC207.020977E-445.012
GTATGGA207.020977E-445.027
CGGGATA253.880605E-545.06
CGAGCGT207.020977E-445.040
TAGGAGA207.020977E-445.010
ACTCATG207.020977E-445.022