##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546330_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	221955
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.10550336779978	31.0	31.0	33.0	30.0	34.0
2	31.443693541483633	31.0	31.0	34.0	30.0	34.0
3	31.548102092766552	31.0	31.0	34.0	30.0	34.0
4	35.394246581514274	37.0	35.0	37.0	33.0	37.0
5	35.29704219323737	37.0	35.0	37.0	33.0	37.0
6	35.3317609425334	37.0	35.0	37.0	33.0	37.0
7	35.739906737852266	37.0	35.0	37.0	35.0	37.0
8	35.647856547498364	37.0	35.0	37.0	35.0	37.0
9	37.39291297785587	39.0	37.0	39.0	35.0	39.0
10	36.886936541190785	39.0	37.0	39.0	32.0	39.0
11	36.57689621770179	39.0	35.0	39.0	32.0	39.0
12	35.802703250658915	37.0	35.0	39.0	31.0	39.0
13	35.40886215674348	37.0	35.0	39.0	30.0	39.0
14	36.31898357775225	38.0	35.0	40.0	31.0	41.0
15	36.63011421234034	38.0	35.0	40.0	31.0	41.0
16	36.754229460926766	38.0	35.0	40.0	32.0	41.0
17	36.68903606586921	38.0	35.0	40.0	32.0	41.0
18	36.62220269874524	38.0	35.0	40.0	31.0	41.0
19	36.53165281250704	38.0	35.0	40.0	31.0	41.0
20	36.37447230294429	38.0	35.0	40.0	31.0	41.0
21	36.210844540560025	38.0	35.0	40.0	31.0	41.0
22	36.092275461242146	37.0	34.0	40.0	30.0	41.0
23	36.012313306751366	37.0	34.0	40.0	30.0	41.0
24	35.98823635421594	37.0	34.0	40.0	30.0	41.0
25	35.916379446284154	37.0	34.0	40.0	30.0	41.0
26	35.78064472528215	37.0	34.0	40.0	30.0	41.0
27	35.65360546056633	37.0	34.0	40.0	30.0	41.0
28	35.63373206280552	37.0	34.0	40.0	29.0	41.0
29	35.5941654839945	37.0	34.0	40.0	29.0	41.0
30	35.448338627199206	36.0	34.0	40.0	29.0	41.0
31	35.24417111576671	36.0	34.0	40.0	29.0	41.0
32	35.072568763938634	36.0	34.0	40.0	27.0	41.0
33	34.88736906129621	36.0	34.0	40.0	27.0	41.0
34	34.696974611970894	36.0	34.0	40.0	25.0	41.0
35	34.48182739744543	36.0	33.0	40.0	24.0	41.0
36	34.300439278232076	36.0	33.0	40.0	24.0	41.0
37	34.096893514451125	36.0	33.0	40.0	23.0	41.0
38	34.06104390529612	35.0	33.0	40.0	23.0	41.0
39	34.0201437228267	35.0	33.0	40.0	23.0	41.0
40	33.80345565542565	35.0	33.0	40.0	23.0	41.0
41	33.70646752720146	35.0	33.0	40.0	23.0	41.0
42	33.567831317158884	35.0	33.0	40.0	23.0	41.0
43	33.505422270280015	35.0	32.0	40.0	23.0	41.0
44	33.36042441035345	35.0	32.0	39.0	22.0	41.0
45	33.29597440922709	35.0	32.0	39.0	22.0	41.0
46	33.220963708859905	35.0	32.0	39.0	22.0	41.0
47	33.1405690342637	35.0	32.0	39.0	22.0	41.0
48	33.07097835146764	35.0	32.0	39.0	22.0	41.0
49	33.073330179540896	35.0	32.0	39.0	22.0	41.0
50	32.928990110608005	35.0	32.0	39.0	21.0	40.0
51	32.70683697145818	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	3.0
15	13.0
16	34.0
17	78.0
18	158.0
19	321.0
20	529.0
21	793.0
22	1267.0
23	1717.0
24	2295.0
25	3193.0
26	4073.0
27	4933.0
28	5255.0
29	5912.0
30	7099.0
31	8332.0
32	10177.0
33	13384.0
34	22411.0
35	29587.0
36	16672.0
37	20436.0
38	27760.0
39	35511.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.012998130251624	23.712013696470006	29.098691176139308	14.17629699713906
2	30.32461534995832	25.468676082989795	30.50438151877633	13.702327048275551
3	29.176634903471427	25.266833367123965	31.59739586853191	13.9591358608727
4	26.018787592079473	27.824559032236262	30.739564326102137	15.417089049582122
5	23.24615349958325	31.87808339528283	29.91462233335586	14.961140771778064
6	21.053366673424794	39.80897028676984	27.795724358541147	11.341938681264221
7	86.40940731229303	4.468923880966863	7.083868351692911	2.0378004550471944
8	88.07686242706855	3.3655470703520987	6.213421639521525	2.344168863057827
9	83.62415805005519	4.917212948570656	8.16516861525985	3.2934603861143024
10	50.991417179157935	22.765875965848934	13.640602824896938	12.60210403009619
11	43.54666486449956	21.193034624135525	20.557770719289948	14.70252979207497
12	39.10747674078079	21.005609245117256	24.715370232704828	15.171543781397128
13	22.501407943051518	36.70699015566218	25.17897772070915	15.612624180577145
14	17.223311031515397	38.181613390101596	28.1962559978374	16.398819580545606
15	15.810412020454597	22.323894483115946	45.29071208127773	16.574981415151722
16	17.69547881327296	17.551305444797368	43.45340271676692	21.29981302516276
17	17.517965353337388	18.855173345948504	29.04958212250231	34.5772791782118
18	23.306526097632403	21.95625239350319	31.98576287986303	22.751458629001377
19	28.506228740059925	24.442341916154177	24.638778130702168	22.412651213083734
20	31.366267937194475	22.183325448852244	24.082359036741682	22.3680475772116
21	24.454056002342817	26.208465679980176	26.62206303079453	22.715415286882475
22	24.90459777882904	24.241850825617806	24.634723254713794	26.218828140839364
23	22.093217093555	28.149850194859315	24.018832646257124	25.738100065328556
24	23.213714491676242	23.17181410646302	34.292536775472506	19.32193462638823
25	20.819535491428443	23.811583429073462	32.13534274965646	23.233538329841636
26	20.201392174089342	29.703318240183822	26.15935662634318	23.93593295938366
27	20.117591403662903	28.81079498096461	28.065148340879908	23.006465274492577
28	17.517064269784417	26.748214728210673	35.08549030208826	20.64923069991665
29	18.586650447162715	23.81879209749724	34.329481201144375	23.26507625419567
30	21.559325088418824	26.806785159153883	30.16377193575274	21.47011781667455
31	28.563898087450156	25.466423374107368	23.778693879389966	22.19098465905251
32	29.65105539411142	24.957311166677933	25.10508886936541	20.286544569845237
33	27.08612106057534	25.997612128584624	25.014529972291683	21.901736838548356
34	21.790903561532744	26.152598499695884	27.603793561758017	24.452704377013358
35	21.36424049920029	23.89268094884098	29.510486359847715	25.232592192111014
36	28.123268230046634	24.96587146043117	25.216823229933993	21.694037079588206
37	22.212160122547363	28.837376945777297	28.058390214232613	20.892072717442726
38	23.170462481133562	28.919826090874277	23.01727827712825	24.892433150863912
39	22.019778783987746	27.97188619314726	26.30352999481877	23.704805028046223
40	24.457660336554707	24.14092946768489	25.48354396161384	25.917866234146565
41	19.420603275438715	23.369601946340474	27.309589781712507	29.9002049965083
42	23.874659277781532	25.770539073235565	24.062985740352776	26.291815908630127
43	23.36194273614021	25.90209727196954	25.521840012615172	25.21411997927508
44	22.055371584330157	28.55128291770854	25.734045189340183	23.659300308621116
45	19.06062039602622	31.856907931787976	23.834110517897773	25.24836115428803
46	23.507918271721746	29.11896555608119	25.727737604469375	21.645378567727693
47	23.028091279763917	26.43734090243518	26.809488409812797	23.725079407988105
48	23.693090941857584	23.594422292807103	28.025050122772633	24.687436642562684
49	21.649433443716067	23.214165033452726	30.182244148588676	24.954157374242527
50	20.55416638507806	28.831519902682977	26.25892635894663	24.355387353292336
51	19.57288639589106	30.69631231555946	24.20175260751053	25.52904868103895
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	84.0
1	158.0
2	232.0
3	878.5
4	1525.0
5	1139.0
6	753.0
7	778.5
8	804.0
9	844.0
10	884.0
11	858.5
12	833.0
13	874.5
14	916.0
15	851.0
16	786.0
17	753.0
18	720.0
19	677.0
20	634.0
21	672.0
22	710.0
23	691.0
24	672.0
25	802.0
26	1087.5
27	1243.0
28	1670.5
29	2098.0
30	2230.5
31	2363.0
32	2815.0
33	3267.0
34	3989.0
35	4711.0
36	4905.0
37	5099.0
38	5872.0
39	6645.0
40	9493.5
41	12342.0
42	14841.5
43	17341.0
44	18033.0
45	18725.0
46	17617.5
47	16510.0
48	16722.0
49	16934.0
50	17624.0
51	18314.0
52	17362.5
53	16411.0
54	14905.0
55	13399.0
56	12360.0
57	11321.0
58	11158.5
59	10996.0
60	10244.5
61	9493.0
62	8733.0
63	7973.0
64	6771.0
65	5569.0
66	4627.5
67	3686.0
68	3251.0
69	2816.0
70	2226.0
71	1636.0
72	1398.5
73	1161.0
74	907.0
75	513.0
76	373.0
77	288.0
78	203.0
79	153.0
80	103.0
81	73.0
82	43.0
83	28.0
84	13.0
85	13.5
86	14.0
87	9.5
88	5.0
89	4.5
90	4.0
91	3.0
92	2.0
93	3.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	221955.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.88238606924827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.73163615143336	21.84361694938163
2	5.975636443212489	3.690838232975153
3	3.2533372237216427	3.014124484692843
4	2.367787584798308	2.9249172129485705
5	1.8863520315121451	2.912752584983443
6	1.6310452987088775	3.022234236669595
7	1.4647311984827485	3.166407605145187
8	1.3596907141294041	3.3592394854812913
9	1.1627398059668832	3.23173616273569
>10	10.133488948865708	51.45547520893875
>50	0.026260121088336127	0.5667815548196706
>100	0.0043766868480560215	0.18066725237097608
>500	0.002917791232037348	0.6312090288572008
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	788	0.35502691987114504	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	613	0.2761821089860557	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04325201054267757	0.0
2	0.0	0.0	0.0	0.17435966750016896	0.0
3	0.0	0.0	0.0	0.25680881259714805	0.0
4	0.0	0.0	0.0	0.3978283886373364	0.0
5	0.0	0.0	0.0	0.681669707823658	0.0
6	0.0	0.0	0.0	0.9722691536572728	0.0
7	0.0	0.0	0.0	1.176815120182019	0.0
8	0.0	0.0	0.0	1.7170147101890023	0.0
9	0.0	0.0	0.0	1.9567029352796739	0.0
10	0.0	0.0	0.0	2.3833659976121284	0.0
11	0.0	0.0	0.0	2.6640535243630468	0.0
12	0.0	0.0	0.0	2.9384334662431573	0.0
13	0.0	0.0	0.0	3.04431078371742	0.0
14	0.0	0.0	0.0	3.0835079182717218	0.0
15	0.0	0.0	0.0	3.1411772656619585	0.0
16	0.0	0.0	0.0	3.2903065936788987	0.0
17	0.0	0.0	0.0	3.4615124687436643	0.0
18	0.0	0.0	0.0	3.697596359622446	0.0
19	0.0	0.0	0.0	3.804374760649681	0.0
20	0.0	0.0	0.0	3.9188123718771823	0.0
21	0.0	0.0	0.0	4.053524363046564	0.0
22	0.0	0.0	0.0	4.190939604874862	0.0
23	0.0	0.0	0.0	4.37025523191638	0.0
24	0.0	0.0	0.0	4.503615597756302	0.0
25	0.0	0.0	0.0	4.611295082336509	0.0
26	0.0	0.0	0.0	4.696898019868892	0.0
27	0.0	0.0	0.0	4.7892590840485685	0.0
28	0.0	0.0	0.0	4.897389110405262	0.0
29	0.0	0.0	0.0	5.009574012750332	0.0
30	0.0	0.0	0.0	5.132121375954585	0.0
31	0.0	0.0	0.0	5.264130116465049	0.0
32	0.0	0.0	0.0	5.372710684598229	0.0
33	0.0	0.0	0.0	5.493455880696538	0.0
34	0.0	0.0	0.0	5.61375053501836	0.0
35	0.0	0.0	0.0	5.77008853145908	0.0
36	0.0	0.0	0.0	5.903448897299002	0.0
37	0.0	0.0	0.0	6.045369556892163	0.0
38	0.0	0.0	0.0	6.19359780135613	0.0
39	0.0	0.0	0.0	6.329661417854971	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACAC	20	7.020977E-4	45.0	26
ACGCCGG	35	1.2060082E-7	45.0	27
AGTCACG	20	7.020977E-4	45.0	1
GATCGAG	20	7.020977E-4	45.0	1
CTCCGAC	25	3.880605E-5	45.0	23
GTTTACG	25	3.880605E-5	45.0	1
CGTTGCA	20	7.020977E-4	45.0	33
CCGGATA	20	7.020977E-4	45.0	20
TAAGTTA	20	7.020977E-4	45.0	42
GTGATAG	20	7.020977E-4	45.0	1
ATTCGCT	20	7.020977E-4	45.0	39
TAAGTCA	20	7.020977E-4	45.0	12
ACGGGTG	20	7.020977E-4	45.0	5
TCTAGAG	45	3.8198777E-10	45.0	1
GACCCAC	20	7.020977E-4	45.0	12
GTATGGA	20	7.020977E-4	45.0	27
CGGGATA	25	3.880605E-5	45.0	6
CGAGCGT	20	7.020977E-4	45.0	40
TAGGAGA	20	7.020977E-4	45.0	10
ACTCATG	20	7.020977E-4	45.0	22
>>END_MODULE