FastQCFastQC Report
Thu 26 May 2016
SRR1546329_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546329_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1297680
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63140.48656063128043897No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21560.1661426545835645No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15660.12067690031440725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAC351.2120836E-745.00000436
CCTCATA253.8910854E-545.026
GCTAACG551.8189894E-1245.01
CGTTTTT51050.042.7081261
CTAACGG750.042.02
CGTAAGG1650.040.909092
ATTTACG403.458681E-739.3751
CTAAGCG403.458681E-739.3751
CGGATAA403.458681E-739.37521
CGGTACG403.458681E-739.37534
TTTACGG1900.039.0789452
ATGACGG1850.038.9189222
ACGGGAA4050.038.8888855
CGCTCGA356.2489107E-638.5714336
CGCGTAG356.2489107E-638.5714313
TACGGGA4500.038.54
TTGTACG659.094947E-1238.076921
GCGAGAC2700.037.521
TATTAGG4500.037.52
CACGACC2650.037.3584927
Produced by FastQC (version 0.11.5)