##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546329_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1297680
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.137946180876643	31.0	31.0	33.0	30.0	34.0
2	31.47778265828247	31.0	31.0	34.0	30.0	34.0
3	31.575738240552372	31.0	31.0	34.0	30.0	34.0
4	35.429702237839834	37.0	35.0	37.0	33.0	37.0
5	35.345718513038655	37.0	35.0	37.0	33.0	37.0
6	35.376023364774056	37.0	35.0	37.0	33.0	37.0
7	35.780177701744655	37.0	35.0	37.0	35.0	37.0
8	35.673642962825966	37.0	35.0	37.0	35.0	37.0
9	37.39448400221934	39.0	37.0	39.0	35.0	39.0
10	36.89569154182849	39.0	37.0	39.0	32.0	39.0
11	36.62323916527958	39.0	35.0	39.0	32.0	39.0
12	36.06938690586277	37.0	35.0	39.0	32.0	39.0
13	35.822356821404355	38.0	35.0	39.0	31.0	39.0
14	36.76875809136305	39.0	35.0	41.0	31.0	41.0
15	36.994428518587014	39.0	35.0	41.0	32.0	41.0
16	37.041408513655135	39.0	35.0	41.0	32.0	41.0
17	36.959681893841314	38.0	35.0	41.0	32.0	41.0
18	36.890136243141605	38.0	35.0	40.0	31.0	41.0
19	36.79357776955798	38.0	35.0	40.0	31.0	41.0
20	36.661485882498	38.0	35.0	40.0	31.0	41.0
21	36.49919779914925	38.0	35.0	40.0	31.0	41.0
22	36.397281302015905	38.0	35.0	40.0	31.0	41.0
23	36.316917884224154	38.0	35.0	40.0	30.0	41.0
24	36.28887167868812	38.0	35.0	40.0	30.0	41.0
25	36.22243773503483	38.0	35.0	40.0	30.0	41.0
26	36.122961747117934	38.0	35.0	40.0	30.0	41.0
27	36.004259909993216	38.0	35.0	40.0	30.0	41.0
28	35.961306331298935	38.0	35.0	40.0	30.0	41.0
29	35.875070125146415	38.0	35.0	40.0	30.0	41.0
30	35.710477159238025	37.0	34.0	40.0	29.0	41.0
31	35.521378922384564	37.0	34.0	40.0	29.0	41.0
32	35.36514009617163	37.0	34.0	40.0	27.0	41.0
33	35.16260942605265	37.0	34.0	40.0	27.0	41.0
34	34.940840577029775	37.0	34.0	40.0	25.0	41.0
35	34.73697213488688	37.0	33.0	40.0	24.0	41.0
36	34.556411441957955	37.0	33.0	40.0	24.0	41.0
37	34.39141699032119	37.0	33.0	40.0	23.0	41.0
38	34.312744282103445	37.0	33.0	40.0	23.0	41.0
39	34.25806670365576	36.0	33.0	40.0	23.0	41.0
40	34.057134270390236	36.0	33.0	40.0	23.0	41.0
41	33.964717033475125	36.0	33.0	40.0	23.0	41.0
42	33.84282180506751	36.0	33.0	40.0	22.0	41.0
43	33.79918392824117	36.0	33.0	40.0	23.0	41.0
44	33.61941387707293	35.0	32.0	40.0	22.0	41.0
45	33.59044525614944	35.0	32.0	40.0	22.0	41.0
46	33.48592565193268	35.0	32.0	40.0	22.0	41.0
47	33.41200064730904	35.0	32.0	40.0	22.0	41.0
48	33.33329172060909	35.0	32.0	39.0	21.0	41.0
49	33.32331776709204	35.0	32.0	39.0	21.0	41.0
50	33.199880556069296	35.0	32.0	39.0	21.0	41.0
51	32.99893271068368	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	8.0
12	8.0
13	8.0
14	24.0
15	51.0
16	157.0
17	393.0
18	905.0
19	1696.0
20	2951.0
21	4614.0
22	6617.0
23	9457.0
24	13043.0
25	18398.0
26	23849.0
27	27636.0
28	29999.0
29	33830.0
30	39075.0
31	46437.0
32	56738.0
33	72305.0
34	114977.0
35	148667.0
36	101327.0
37	127701.0
38	179609.0
39	237166.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.39468898341656	22.986252388878615	28.42195302385796	14.197105603846866
2	32.57205166142654	24.781379076505765	29.217603723568214	13.428965538499476
3	29.432911041242832	25.244359164046603	31.322822267431107	13.999907527279454
4	26.00972504777757	27.507783120646078	30.82269897047038	15.659792861105974
5	23.38373096603169	31.446119228161024	29.8041890142408	15.365960791566488
6	21.267338635102647	40.60985759201036	27.342025769064794	10.780778003822206
7	86.36212317366378	4.191171937611738	7.508630787251095	1.9380741014733989
8	87.05220085074903	3.47003883854263	7.3122803772887	2.165479933419641
9	81.7160625115591	5.5284045373281545	9.477220886505147	3.2783120646076074
10	44.94220454965785	25.810831638000124	16.019588804635966	13.22737500770606
11	38.09537019912459	24.020097404598978	22.319986437334318	15.56454595894211
12	33.72510942605265	22.741584982430183	27.413538006288146	16.119767585229024
13	23.712163245176008	31.84698847173417	27.405061340237964	17.035786942851857
14	19.195487331237285	34.64251587448369	28.989041982615127	17.172954811663892
15	18.306516244374578	23.370245360951852	40.804897971764994	17.518340422908576
16	20.30708649281795	20.20251525799889	39.023025707416316	20.467372541766846
17	20.379754639048148	20.05532951112755	29.700311324825847	29.864604524998462
18	23.391051723075027	22.727791134948525	32.531132482584304	21.350024659392147
19	28.432279144319097	23.98102768016768	26.545835645151346	21.040857530361876
20	30.52177732568892	23.585706799827385	25.358331792121326	20.53418408236237
21	24.993989273164416	25.84920781702731	28.436132174341903	20.72067073546637
22	24.50619567227668	23.6275507058751	26.988625855372668	24.87762776647556
23	22.354586646939154	27.60195117440355	26.13679797792984	23.90666420072745
24	22.170257690647926	24.53925466987239	33.87190986992171	19.41857776955798
25	21.686779483385735	24.65839035817767	31.352567659207196	22.302262499229393
26	20.542352506010726	29.26638308365699	27.377550705875098	22.813713704457186
27	20.313097219653535	28.171814314777137	29.21583132975772	22.2992571358116
28	19.361938228222677	26.33430429689908	33.72287466863942	20.580882806238826
29	19.9894426977375	24.345909623327785	33.495777079095	22.168870599839714
30	21.85315331977067	26.223491153443067	31.55878182602799	20.364573700758275
31	27.19268232538068	25.214690832870968	26.67714690832871	20.915479933419643
32	28.02401208310215	25.645151347019297	25.88820048085815	20.442636089020404
33	26.78171814314777	25.77129954996609	26.34809814438074	21.098884162505392
34	22.248397139510512	26.19582639787929	28.68049133838851	22.87528512422169
35	21.694100240429073	26.007259108562973	29.90567782504161	22.39296282596634
36	27.63154244497873	25.656093952284074	26.11984464582948	20.592518956907714
37	22.722473953517046	29.192944331422233	27.38787682633623	20.696704888724494
38	22.894396153134824	29.752018987731955	24.858670858763332	22.494914000369892
39	22.01975833795697	29.05654706861476	26.013963380802664	22.90973121262561
40	24.09731212625609	25.38699833549103	26.831499291042476	23.684190247210406
41	20.547284384439923	24.768587016830036	27.364835706799827	27.319292891930214
42	22.828971703347513	26.225957092657666	25.555529868688737	25.389541335306088
43	22.846541520251527	25.944377658590717	27.023919610381604	24.185161210776155
44	22.29078046976142	27.179042599099933	28.432433265520007	22.097743665618644
45	20.8932094198878	30.259231859934655	25.467911965970043	23.37964675420751
46	22.688875531718146	28.605126071142344	27.376703039270083	21.32929535786943
47	22.411149127674	27.287466863941805	27.739273164416495	22.562110843967695
48	23.18052216262869	24.808196165464523	29.086138339189937	22.92514333271685
49	21.720069662782812	24.4424665556994	30.659561679304602	23.17790210221318
50	20.836569878552496	28.751232969607297	27.59385981135565	22.818337340484558
51	20.979671413599654	29.573392515874485	26.451821712594786	22.995114357931076
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	494.0
1	1046.0
2	1598.0
3	6971.0
4	12344.0
5	9039.5
6	5735.0
7	5781.5
8	5828.0
9	5890.0
10	5952.0
11	5943.0
12	5934.0
13	5835.5
14	5737.0
15	5491.5
16	5246.0
17	5195.0
18	5144.0
19	4881.5
20	4619.0
21	4929.5
22	5240.0
23	5759.0
24	6278.0
25	7003.5
26	9213.5
27	10698.0
28	12497.0
29	14296.0
30	16131.5
31	17967.0
32	21840.0
33	25713.0
34	30383.5
35	35054.0
36	36904.5
37	38755.0
38	44155.0
39	49555.0
40	59520.5
41	69486.0
42	81447.5
43	93409.0
44	95109.0
45	96809.0
46	95915.0
47	95021.0
48	93226.0
49	91431.0
50	94417.5
51	97404.0
52	94060.5
53	90717.0
54	82230.0
55	73743.0
56	69427.5
57	65112.0
58	63986.5
59	62861.0
60	58146.5
61	53432.0
62	48263.5
63	43095.0
64	37502.5
65	31910.0
66	27488.0
67	23066.0
68	19050.0
69	15034.0
70	12779.5
71	10525.0
72	8365.5
73	6206.0
74	5060.5
75	3100.0
76	2285.0
77	1705.5
78	1126.0
79	823.5
80	521.0
81	444.0
82	367.0
83	256.0
84	145.0
85	118.0
86	91.0
87	59.0
88	27.0
89	20.0
90	13.0
91	9.5
92	6.0
93	5.5
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1297680.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.266242089294405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.98036209056237	17.585958626085922
2	5.583251958920835	2.4863607952571707
3	1.9425391997459636	1.2975914426846356
4	1.1483645921843855	1.0227905606540548
5	0.8444756842777654	0.9401650012325637
6	0.6399571101716158	0.8549663965107871
7	0.5285997663024152	0.8238951255383816
8	0.49881831849588876	0.8885447550563376
9	0.41979266445523855	0.8412484584663248
>10	7.458265387421373	42.363333445899606
>50	1.8603996975831008	26.990637554740836
>100	0.09308943798395651	2.9545996438959907
>500	0.0010420459475814032	0.1737089890631243
>1k	6.94697298387602E-4	0.2878650299612416
>5k	3.47348649193801E-4	0.4883341749530573
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6314	0.48656063128043897	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2156	0.1661426545835645	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1566	0.12067690031440725	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03390666420072745	0.0
2	0.0	0.0	0.0	0.1296929905677825	0.0
3	0.0	0.0	0.0	0.18402071388940264	0.0
4	0.0	0.0	0.0	0.2552247087109303	0.0
5	0.0	0.0	0.0	0.43469884717341717	0.0
6	0.0	0.0	0.0	0.621493742679243	0.0
7	0.0	0.0	0.0	0.7332316133407312	0.0
8	0.0	0.0	0.0	1.020128228839159	0.0
9	0.0	0.0	0.0	1.1519789162197152	0.0
10	0.0	0.0	0.0	1.3773041119536404	0.0
11	0.0	0.0	0.0	1.5986221564638432	0.0
12	0.0	0.0	0.0	1.7824887491523334	0.0
13	0.0	0.0	0.0	1.8628629554281486	0.0
14	0.0	0.0	0.0	1.8962301954256828	0.0
15	0.0	0.0	0.0	1.9485543431354417	0.0
16	0.0	0.0	0.0	2.072776031070834	0.0
17	0.0	0.0	0.0	2.2074779606682697	0.0
18	0.0	0.0	0.0	2.3935793107699896	0.0
19	0.0	0.0	0.0	2.4768047592626843	0.0
20	7.706060045619876E-5	0.0	0.0	2.5671968435978054	0.0
21	7.706060045619876E-5	0.0	0.0	2.6847142592935085	0.0
22	7.706060045619876E-5	0.0	0.0	2.80469761420381	0.0
23	7.706060045619876E-5	0.0	0.0	2.9529622094815364	0.0
24	7.706060045619876E-5	0.0	0.0	3.066318352752605	0.0
25	7.706060045619876E-5	0.0	0.0	3.1604864065100795	0.0
26	7.706060045619876E-5	0.0	0.0	3.2503390666420073	0.0
27	7.706060045619876E-5	0.0	0.0	3.346818938413168	0.0
28	7.706060045619876E-5	0.0	0.0	3.4435299919856974	0.0
29	7.706060045619876E-5	0.0	0.0	3.551568953825288	0.0
30	7.706060045619876E-5	0.0	0.0	3.679027186979841	0.0
31	7.706060045619876E-5	0.0	0.0	3.7967757844769126	0.0
32	7.706060045619876E-5	0.0	0.0	3.9021946859009926	0.0
33	7.706060045619876E-5	0.0	0.0	4.013701374761112	0.0
34	7.706060045619876E-5	0.0	0.0	4.131141729856359	0.0
35	7.706060045619876E-5	0.0	0.0	4.2727791134948525	0.0
36	7.706060045619876E-5	0.0	0.0	4.397848468035263	0.0
37	7.706060045619876E-5	0.0	0.0	4.523380186178411	0.0
38	7.706060045619876E-5	0.0	0.0	4.64421120769373	0.0
39	7.706060045619876E-5	0.0	0.0	4.803418408236237	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGTAC	35	1.2120836E-7	45.000004	36
CCTCATA	25	3.8910854E-5	45.0	26
GCTAACG	55	1.8189894E-12	45.0	1
CGTTTTT	5105	0.0	42.708126	1
CTAACGG	75	0.0	42.0	2
CGTAAGG	165	0.0	40.90909	2
ATTTACG	40	3.458681E-7	39.375	1
CTAAGCG	40	3.458681E-7	39.375	1
CGGATAA	40	3.458681E-7	39.375	21
CGGTACG	40	3.458681E-7	39.375	34
TTTACGG	190	0.0	39.078945	2
ATGACGG	185	0.0	38.918922	2
ACGGGAA	405	0.0	38.888885	5
CGCTCGA	35	6.2489107E-6	38.57143	36
CGCGTAG	35	6.2489107E-6	38.57143	13
TACGGGA	450	0.0	38.5	4
TTGTACG	65	9.094947E-12	38.07692	1
GCGAGAC	270	0.0	37.5	21
TATTAGG	450	0.0	37.5	2
CACGACC	265	0.0	37.35849	27
>>END_MODULE