FastQCFastQC Report
Thu 26 May 2016
SRR1546328_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546328_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1231280
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC51150.415421350139692No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51120.4151777012539796No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT39040.31706841660710805No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACGT207.0334825E-445.014
ACGCACG207.0334825E-445.01
CGCATAT253.890969E-545.034
TAAGTCG253.890969E-545.02
TCGATAT351.212029E-745.017
CCGTAGA453.8562575E-1045.018
GTGTCGC207.0334825E-445.035
ACTACCG253.890969E-545.01
TCGTAAG406.8157533E-945.01
CCGAACG253.890969E-545.037
CTTTACG253.890969E-545.01
CACGTTA207.0334825E-445.014
TATACGT253.890969E-545.015
CGTCCAC207.0334825E-445.022
CAATACG351.212029E-745.01
GTACGTC253.890969E-545.019
TACGTCT253.890969E-545.017
GACCGTA302.1656288E-644.99999641
CCTACGT302.1656288E-644.99999644
CGGATAC302.1656288E-644.99999641
Produced by FastQC (version 0.11.5)