FastQCFastQC Report
Thu 26 May 2016
SRR1546327_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546327_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3186460
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC147270.46217432511313494No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145360.45618021252424323No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA207.0351706E-445.0000041
GCGTAAT207.0351706E-445.0000042
TCCGCGA253.892368E-545.029
GTCCGCG253.892368E-545.028
CGAATCG253.892368E-545.016
ACGTCCG453.8562575E-1045.030
ACTCGAT302.1667183E-644.99999614
TTACGTA302.1667183E-644.99999620
CGTAACG302.1667183E-644.99999622
AATCGAC302.1667183E-644.99999635
CGTTTTT120350.043.6539271
CGTTGAT1600.043.59375425
CTCACGA2550.042.3529424
ACGTTAG1550.042.096771
CTATGCG1350.041.6666641
TTCGCGA556.184564E-1140.90909215
CGGGTAT2550.040.5882346
GCACGAC501.0822987E-940.59
ATTCGGC2450.040.40816517
GGCACCG12300.040.24398
Produced by FastQC (version 0.11.5)