##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546327_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3186460
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.10638514213265	31.0	31.0	33.0	30.0	34.0
2	31.42621467082593	31.0	31.0	34.0	30.0	34.0
3	31.498391945921178	31.0	31.0	34.0	30.0	34.0
4	35.34841862129134	37.0	35.0	37.0	33.0	37.0
5	35.242731432373226	37.0	35.0	37.0	33.0	37.0
6	35.27175329362365	37.0	35.0	37.0	32.0	37.0
7	35.78774282432543	37.0	35.0	37.0	35.0	37.0
8	35.71105584253372	37.0	35.0	37.0	35.0	37.0
9	37.44884605486967	39.0	37.0	39.0	35.0	39.0
10	36.8922581799238	39.0	37.0	39.0	32.0	39.0
11	36.53491554891635	39.0	35.0	39.0	32.0	39.0
12	35.72309867376336	37.0	35.0	39.0	31.0	39.0
13	35.33039077848145	37.0	35.0	39.0	30.0	39.0
14	36.220412307074305	38.0	35.0	40.0	30.0	41.0
15	36.55173892030655	38.0	35.0	40.0	31.0	41.0
16	36.69666589255789	38.0	35.0	40.0	32.0	41.0
17	36.63083390345399	38.0	35.0	40.0	31.0	41.0
18	36.54976211846375	38.0	35.0	40.0	31.0	41.0
19	36.44023995280028	37.0	35.0	40.0	31.0	41.0
20	36.30160240517691	37.0	35.0	40.0	31.0	41.0
21	36.1325298293404	37.0	34.0	40.0	31.0	41.0
22	36.018684684571596	37.0	34.0	40.0	30.0	41.0
23	35.97680874701079	37.0	34.0	40.0	30.0	41.0
24	35.928185196111045	37.0	34.0	40.0	30.0	41.0
25	35.87941791204032	37.0	34.0	40.0	30.0	41.0
26	35.75198433371202	37.0	34.0	40.0	30.0	41.0
27	35.6441176101379	37.0	34.0	40.0	30.0	41.0
28	35.624429617820404	36.0	34.0	40.0	30.0	41.0
29	35.61184292286738	36.0	34.0	40.0	30.0	41.0
30	35.479716676186115	36.0	34.0	40.0	29.0	41.0
31	35.2917704913917	36.0	34.0	40.0	29.0	41.0
32	35.08882019545201	36.0	34.0	40.0	27.0	41.0
33	34.882385154685764	36.0	34.0	40.0	27.0	41.0
34	34.67991030799069	36.0	34.0	40.0	25.0	41.0
35	34.51897560302028	36.0	33.0	40.0	25.0	41.0
36	34.308888233337306	36.0	33.0	40.0	24.0	41.0
37	34.12998845113386	36.0	33.0	40.0	23.0	41.0
38	34.08417303214225	35.0	33.0	40.0	23.0	41.0
39	34.06908136301727	36.0	33.0	40.0	23.0	41.0
40	33.87440043182717	35.0	33.0	40.0	23.0	41.0
41	33.82639606334302	35.0	33.0	40.0	23.0	41.0
42	33.717087614468724	35.0	33.0	40.0	23.0	41.0
43	33.64930675420372	35.0	33.0	40.0	23.0	41.0
44	33.442490098730254	35.0	32.0	40.0	22.0	41.0
45	33.425876992022495	35.0	32.0	39.0	23.0	41.0
46	33.35068571392706	35.0	32.0	39.0	22.0	41.0
47	33.296453117252376	35.0	32.0	39.0	22.0	41.0
48	33.251289518776325	35.0	32.0	39.0	22.0	41.0
49	33.28083139283092	35.0	32.0	39.0	22.0	41.0
50	33.111168192916274	35.0	32.0	39.0	21.0	41.0
51	32.8978361567382	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	5.0
13	15.0
14	42.0
15	103.0
16	300.0
17	866.0
18	1965.0
19	3945.0
20	6912.0
21	10909.0
22	16454.0
23	23818.0
24	33942.0
25	47173.0
26	61131.0
27	69882.0
28	76008.0
29	85265.0
30	99763.0
31	121229.0
32	148638.0
33	190808.0
34	319836.0
35	439774.0
36	230437.0
37	276184.0
38	384101.0
39	536855.0
40	94.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.03570733666828	23.849569741970715	30.356885069952234	13.757837851408775
2	30.042460912737017	26.426316351060425	30.537430251752728	12.993792484449829
3	27.762313037037966	25.153273538660457	33.09735568624744	13.987057738054142
4	24.19085756607646	27.981521814176237	32.35195169561206	15.475668924135247
5	23.12274436208206	31.9072262008624	31.032776184229522	13.937253252826022
6	20.378445045599193	40.3121332136603	28.318447430691112	10.990974310049397
7	86.18055773491587	4.492446162826459	7.601005504541089	1.7259905977165881
8	87.52747563126478	3.2758296040119754	6.742968686253711	2.4537260784695243
9	82.59328533858891	5.2438442660507265	9.053400952781457	3.109469442578912
10	49.446533143362856	27.336354449765572	12.807598400733102	10.409514006138473
11	42.863271467396416	20.477520508652237	23.528429668032864	13.13077835591848
12	37.84971410279746	21.619038054769245	26.132636216993156	14.398611625440145
13	21.434005134224186	38.481418250974436	26.413480790595205	13.671095824206173
14	16.10489383202676	40.87793350614789	28.481857610012362	14.535315051812983
15	13.936029324077504	23.01183758779335	47.91640252819744	15.135730559931712
16	15.933669338388054	19.35153744280487	44.90268197309867	19.8121112457084
17	16.733522466938233	19.23903014630656	29.531046992587385	34.49640039416782
18	22.065238540574807	22.189514382731936	34.431971529534344	21.313275547158916
19	27.28563986367317	24.83963395115583	26.26952794009653	21.605198245074472
20	30.5173766499501	22.95500335795836	25.106167973236758	21.42145201885478
21	22.52766392799533	27.910345650031697	28.08913339568047	21.4728570262925
22	22.885961223426623	24.283154346830024	26.719525743301343	26.11135868644201
23	19.873778424960616	29.939462601131034	25.10108396151215	25.0856750123962
24	20.592820873320235	23.860898928591602	37.492546587749416	18.053733610338746
25	17.402666281704462	25.532942513008166	34.583770077138894	22.480621128148478
26	17.997244591176415	32.919352510309245	28.079436114057604	21.003966784456733
27	18.85192345110248	31.08418119166724	29.337289656860595	20.72660570036969
28	15.777351669250518	27.808508501597384	37.589268341670696	18.824871487481406
29	16.12406871575353	24.14632538930349	36.74830376028571	22.98130213465727
30	18.806920532503156	28.968824337980077	31.601589224405767	20.622665905111003
31	26.98172894057983	26.62678960350985	24.87500235370913	21.51647910220119
32	28.172046722695406	28.067761716764057	25.01917488372677	18.741016676813768
33	25.942864495396144	27.677297063198658	25.064177802326093	21.315660639079105
34	18.849695273124407	29.152853009295583	28.279909366507034	23.71754235107298
35	19.576897246474143	26.899506034910214	29.49737326060895	24.02622345800669
36	27.041889745987714	25.958650037973175	27.10452979168105	19.894930424358066
37	20.143764553768133	30.751806079473777	28.82929646064912	20.275132906108972
38	20.178913276802472	31.800022595607665	24.17573106205633	23.845333065533538
39	20.01045046854503	31.09789547020832	27.197297314260965	21.694356746985683
40	21.964185961851083	26.69614556592582	26.129592086516073	25.210076385707026
41	17.972106977649176	25.062232069443834	26.549211350526917	30.41644960238007
42	22.228429040377094	25.820063644294923	24.959170992261004	26.99233632306698
43	22.15235716123849	26.4765915781149	26.212505413531	25.15854584711561
44	20.11420196707318	30.22040132309836	27.44415432800035	22.22124238182811
45	17.75735455646705	34.189100129924746	23.974441857107887	24.079103456500317
46	22.04188974598771	31.073040301776896	26.42349190010231	20.46157805213309
47	21.24868976858332	28.18566685287121	26.522849808251163	24.042793570294307
48	21.9780885371227	24.617757637001564	28.94148992926319	24.46266389661254
49	20.97004199017091	22.956384200648998	31.875247139458835	24.198326669721258
50	18.87750042366764	30.104002560835536	27.558513209015643	23.459983806481173
51	18.120170973431332	31.810598595306388	24.814245275321202	25.254985155941075
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1744.0
1	2405.0
2	3066.0
3	13899.5
4	24733.0
5	18428.0
6	12123.0
7	12941.0
8	13759.0
9	14663.5
10	15568.0
11	15765.5
12	15963.0
13	15527.0
14	15091.0
15	14925.5
16	14760.0
17	13924.0
18	13088.0
19	13040.5
20	12993.0
21	12697.0
22	12401.0
23	13312.5
24	14224.0
25	14231.5
26	17917.5
27	21596.0
28	25473.0
29	29350.0
30	33355.5
31	37361.0
32	43591.0
33	49821.0
34	61038.0
35	72255.0
36	78806.0
37	85357.0
38	104770.0
39	124183.0
40	170170.0
41	216157.0
42	263561.0
43	310965.0
44	333445.0
45	355925.0
46	341159.0
47	326393.0
48	299680.5
49	272968.0
50	253342.5
51	233717.0
52	217294.0
53	200871.0
54	182860.5
55	164850.0
56	148522.0
57	132194.0
58	119285.0
59	106376.0
60	100073.5
61	93771.0
62	77318.5
63	60866.0
64	49610.5
65	38355.0
66	33223.0
67	28091.0
68	22306.5
69	16522.0
70	13310.0
71	10098.0
72	8752.0
73	7406.0
74	5389.5
75	2616.0
76	1859.0
77	1552.0
78	1245.0
79	891.5
80	538.0
81	333.0
82	128.0
83	94.0
84	60.0
85	43.5
86	27.0
87	19.5
88	12.0
89	7.0
90	2.0
91	8.0
92	14.0
93	8.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3186460.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.70667586386095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.17963701575117	14.811878046718235
2	8.973519998849211	3.3572945995269214
3	3.197449667233096	1.7944096354781873
4	1.5380051823913194	1.1508385769573102
5	0.9005488245719723	0.8423137480424432
6	0.5716398589510349	0.6416088931356122
7	0.41690516210504913	0.5459236813428693
8	0.3065668041887812	0.4587876669263407
9	0.23859142672731748	0.40169272353156527
>10	2.3271437166361686	9.965196445687925
>50	0.8560316095129356	11.722667946436447
>100	1.4807188244940717	51.630223725407674
>500	0.011374459940865536	1.2932794247743877
>1k	0.0015279125293699977	0.4545516884020287
>5k	0.0	0.0
>10k+	3.395361176377772E-4	0.9293331976321222
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	14727	0.46217432511313494	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14536	0.45618021252424323	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08875052566170609	0.0
2	0.0	0.0	0.0	0.386604570589306	0.0
3	3.1382788423517E-5	0.0	0.0	0.5355786672357412	0.0
4	3.1382788423517E-5	0.0	0.0	0.795428155382462	0.0
5	3.1382788423517E-5	0.0	0.0	1.3362477482849306	0.0
6	3.1382788423517E-5	0.0	0.0	1.8320958053764993	0.0
7	3.1382788423517E-5	0.0	0.0	2.150317279990962	0.0
8	3.1382788423517E-5	0.0	0.0	3.029223652579979	0.0
9	3.1382788423517E-5	0.0	0.0	3.3900001882967303	0.0
10	3.1382788423517E-5	0.0	0.0	4.022991030799068	0.0
11	3.1382788423517E-5	0.0	0.0	4.55009634516046	0.0
12	3.1382788423517E-5	0.0	0.0	5.0315710851540585	0.0
13	3.1382788423517E-5	0.0	0.0	5.21246147762721	0.0
14	3.1382788423517E-5	0.0	0.0	5.27742384966389	0.0
15	3.1382788423517E-5	0.0	0.0	5.389931146162199	0.0
16	3.1382788423517E-5	0.0	0.0	5.643943435662146	0.0
17	3.1382788423517E-5	0.0	0.0	5.956013883745599	0.0
18	3.1382788423517E-5	0.0	0.0	6.404850523778739	0.0
19	3.1382788423517E-5	0.0	0.0	6.5978860553717915	0.0
20	3.1382788423517E-5	0.0	0.0	6.803443319545829	0.0
21	3.1382788423517E-5	0.0	0.0	7.053626908858106	0.0
22	3.1382788423517E-5	0.0	0.0	7.317210948827225	0.0
23	3.1382788423517E-5	0.0	0.0	7.622659628553317	0.0
24	3.1382788423517E-5	0.0	0.0	7.847799752703628	0.0
25	3.1382788423517E-5	0.0	0.0	8.018773184034949	0.0
26	3.1382788423517E-5	0.0	0.0	8.200761974102923	0.0
27	3.1382788423517E-5	0.0	0.0	8.375313043314524	0.0
28	3.1382788423517E-5	0.0	0.0	8.55667417761403	0.0
29	3.1382788423517E-5	0.0	0.0	8.755295845546469	0.0
30	3.1382788423517E-5	0.0	0.0	9.020888383974693	0.0
31	3.1382788423517E-5	0.0	0.0	9.24948061485159	0.0
32	3.1382788423517E-5	0.0	0.0	9.457956478349015	0.0
33	3.1382788423517E-5	0.0	0.0	9.657739309453124	0.0
34	3.1382788423517E-5	0.0	0.0	9.881467208124377	0.0
35	3.1382788423517E-5	0.0	0.0	10.151139509047658	0.0
36	3.1382788423517E-5	0.0	0.0	10.38716946078093	0.0
37	3.1382788423517E-5	0.0	0.0	10.615008504735663	0.0
38	3.1382788423517E-5	0.0	0.0	10.834876320430823	0.0
39	3.1382788423517E-5	0.0	0.0	11.067265868706967	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAA	20	7.0351706E-4	45.000004	1
GCGTAAT	20	7.0351706E-4	45.000004	2
TCCGCGA	25	3.892368E-5	45.0	29
GTCCGCG	25	3.892368E-5	45.0	28
CGAATCG	25	3.892368E-5	45.0	16
ACGTCCG	45	3.8562575E-10	45.0	30
ACTCGAT	30	2.1667183E-6	44.999996	14
TTACGTA	30	2.1667183E-6	44.999996	20
CGTAACG	30	2.1667183E-6	44.999996	22
AATCGAC	30	2.1667183E-6	44.999996	35
CGTTTTT	12035	0.0	43.653927	1
CGTTGAT	160	0.0	43.593754	25
CTCACGA	255	0.0	42.35294	24
ACGTTAG	155	0.0	42.09677	1
CTATGCG	135	0.0	41.666664	1
TTCGCGA	55	6.184564E-11	40.909092	15
CGGGTAT	255	0.0	40.588234	6
GCACGAC	50	1.0822987E-9	40.5	9
ATTCGGC	245	0.0	40.408165	17
GGCACCG	1230	0.0	40.2439	8
>>END_MODULE