FastQCFastQC Report
Thu 26 May 2016
SRR1546325_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546325_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3594680
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169140.4705286701458823No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC112820.31385269342472766No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT56180.15628651229038468No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA207.035291E-445.00000428
CCGTACC253.8924678E-545.00000433
TGGTCGA253.8924678E-545.00000410
CGCGATA302.1667965E-644.99999623
ACGGGTA1450.043.4482735
CGTTTTT154300.043.1189271
TGATGCG1050.042.8571471
TCGATAG2050.041.7073171
CGGTCTA3150.041.42857431
TAATGCG1800.041.2500041
TACGGGA8750.040.8857154
GCTACGA7750.040.6451610
GTATCGA950.040.2631622
TAAACGG2350.040.2127652
GCGATAT4200.040.1785749
CGTAGGT451.9303116E-840.00000421
CGTAGAC451.9303116E-840.00000434
GCGTAAG850.039.7058831
GCGTACA403.4609548E-739.3750049
ACCGCTC1200.039.37499618
Produced by FastQC (version 0.11.5)