##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546325_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3594680
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.135409827856723	31.0	31.0	33.0	30.0	34.0
2	31.461944874091714	31.0	31.0	34.0	30.0	34.0
3	31.52975591707746	31.0	31.0	34.0	30.0	34.0
4	35.38376962622542	37.0	35.0	37.0	33.0	37.0
5	35.28532442387083	37.0	35.0	37.0	33.0	37.0
6	35.32236304761481	37.0	35.0	37.0	33.0	37.0
7	35.783290584975575	37.0	35.0	37.0	35.0	37.0
8	35.70472642905627	37.0	35.0	37.0	35.0	37.0
9	37.45967680016024	39.0	37.0	39.0	35.0	39.0
10	36.93302018538507	39.0	37.0	39.0	32.0	39.0
11	36.61473594311594	39.0	35.0	39.0	32.0	39.0
12	35.8031151590684	37.0	35.0	39.0	31.0	39.0
13	35.40130359308756	37.0	35.0	39.0	30.0	39.0
14	36.28619877151791	38.0	35.0	40.0	30.0	41.0
15	36.62457576195934	38.0	35.0	40.0	31.0	41.0
16	36.74427153460113	38.0	35.0	40.0	32.0	41.0
17	36.6984118196891	38.0	35.0	40.0	32.0	41.0
18	36.601674140674554	38.0	35.0	40.0	31.0	41.0
19	36.49816117150901	37.0	35.0	40.0	31.0	41.0
20	36.3304789299743	37.0	35.0	40.0	31.0	41.0
21	36.17913778138805	37.0	35.0	40.0	31.0	41.0
22	36.06282923653844	37.0	35.0	40.0	30.0	41.0
23	36.025391968130684	37.0	34.0	40.0	30.0	41.0
24	35.9605619971736	37.0	34.0	40.0	30.0	41.0
25	35.88857033171242	37.0	34.0	40.0	30.0	41.0
26	35.773135856321005	37.0	34.0	40.0	30.0	41.0
27	35.64586806057841	37.0	34.0	40.0	30.0	41.0
28	35.63299653933034	36.0	34.0	40.0	30.0	41.0
29	35.60264446348493	36.0	34.0	40.0	30.0	41.0
30	35.47150455673384	36.0	34.0	40.0	29.0	41.0
31	35.2339791024514	36.0	34.0	40.0	29.0	41.0
32	35.02733817752902	36.0	34.0	40.0	27.0	41.0
33	34.81771145136702	36.0	34.0	40.0	26.0	41.0
34	34.62487175492672	36.0	34.0	40.0	25.0	41.0
35	34.427524842266905	36.0	33.0	40.0	24.0	41.0
36	34.18392958483091	36.0	33.0	40.0	23.0	41.0
37	33.99950621473956	36.0	33.0	40.0	23.0	41.0
38	33.96267873635484	35.0	33.0	40.0	23.0	41.0
39	33.923792938453495	35.0	33.0	40.0	23.0	41.0
40	33.72190959974184	35.0	33.0	40.0	23.0	41.0
41	33.64911035196457	35.0	33.0	40.0	22.0	41.0
42	33.53686670301668	35.0	33.0	40.0	22.0	41.0
43	33.49263606218078	35.0	32.0	40.0	22.0	41.0
44	33.31644318826711	35.0	32.0	40.0	21.0	41.0
45	33.27049445291375	35.0	32.0	39.0	22.0	41.0
46	33.18959684867638	35.0	32.0	39.0	21.0	41.0
47	33.12413483258593	35.0	32.0	39.0	20.0	41.0
48	33.07278784203323	35.0	32.0	39.0	20.0	41.0
49	33.09533866714144	35.0	32.0	39.0	20.0	41.0
50	32.92410590094251	35.0	32.0	39.0	20.0	41.0
51	32.70159151857745	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	3.0
12	14.0
13	16.0
14	44.0
15	156.0
16	439.0
17	1103.0
18	2482.0
19	4962.0
20	8501.0
21	13532.0
22	19943.0
23	28341.0
24	40178.0
25	55475.0
26	72074.0
27	82474.0
28	87337.0
29	96273.0
30	111186.0
31	132976.0
32	164297.0
33	211316.0
34	358468.0
35	492034.0
36	252733.0
37	308988.0
38	438008.0
39	611234.0
40	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.89666952273916	22.885069046479796	29.74796087551604	14.470300555265004
2	30.96948824373797	26.04816005875349	30.642699767434095	12.339651930074444
3	27.814742897837917	24.86187922151624	33.89603525209476	13.42734262855108
4	25.20597104610146	27.584819789244104	31.815293711818576	15.393915452835857
5	22.569046479797926	32.29458533165678	31.388551971246397	13.747816217298897
6	20.68451155596604	39.77772708558203	28.657293556032805	10.88046780241913
7	85.5069992321987	4.737528792548988	7.914139784347982	1.8413321909043365
8	87.43201063794274	3.3855308400191393	7.052644463484928	2.1298140585531953
9	82.93653398911725	5.001557857723079	9.151273548688618	2.910634604471052
10	49.78078716325236	24.98631310714723	14.102479219290728	11.13042051030968
11	43.66430391578666	20.007538918624192	21.92222951695283	14.405927648636318
12	38.64713966194488	21.649576596525975	25.59654823238786	14.106735509141286
13	21.883505624979136	39.00580858379605	25.40757452680072	13.703111264424091
14	16.232738380050517	40.5668376600977	28.083779362836193	15.116644597015592
15	14.16626792927326	22.70508084168827	48.19836536214628	14.930285866892184
16	15.91980370992689	18.34077024936851	44.75079840208308	20.98862763862152
17	16.808394627616366	18.694097944740562	29.392602401326407	35.10490502631667
18	21.365378837615587	21.376116928349674	35.83929584830916	21.41920838572557
19	27.516802608298928	24.399529304416525	26.84347424527357	21.24019384201097
20	30.79125262888491	22.130954632957593	25.990157677456686	21.087635060700812
21	22.775128801451032	26.624845605172087	27.973672204479954	22.626353388896923
22	22.858390732972058	23.80069992321987	26.010882748951232	27.330026594856843
23	20.116366408136468	28.243626692779326	25.372049806936918	26.26795709214728
24	20.84766933357072	23.25578354679693	37.86495599051932	18.03159112911302
25	18.03793383555699	25.351324735442375	34.53158556533544	22.079155863665196
26	19.054825464297238	32.35108549300633	26.78213359742731	21.81195544526912
27	18.40636718706533	31.073447427865624	29.67621596359064	20.843969421478405
28	16.016084880990796	27.004044866302422	38.040854818787764	18.939015433919014
29	16.497490736310326	23.57322487676233	36.89257458243849	23.036709804488854
30	19.69941691610936	28.008918735464633	32.565819488800116	19.72584485962589
31	27.8236449419698	26.661510899440284	25.50346623343385	20.011377925156065
32	28.541956446749083	26.34579434052544	25.917661655557655	19.194587557167814
33	26.48027084469271	27.73315009959162	25.45408770738981	20.33249134832586
34	19.931287346857022	27.64229361167058	28.301907262955257	24.124511778517142
35	20.219991765609176	26.289099446960506	29.848387060878856	23.64252172655146
36	28.651952329553676	25.493924354879987	26.445107770371774	19.409015545194567
37	21.39444957548377	30.71469504935071	28.474440005786327	19.416415369379195
38	21.044181957782055	30.931932745056585	25.320390132084082	22.703495165077282
39	20.971268652564344	30.625702426919784	26.872934447572522	21.53009447294335
40	23.49252228292922	25.554569530528447	26.771562420020693	24.181345766521638
41	17.839334794752247	24.92283040493173	27.61797990363537	29.619854896680653
42	22.015255878131015	26.45567894777838	25.399813057073228	26.12925211701737
43	21.9148296927682	27.148035430135646	26.929907530016578	24.007227347079574
44	20.848865545750943	29.23550914128657	28.24743788042329	21.668187432539195
45	18.56326571488978	34.62230295881692	23.224598573447427	23.589832752845872
46	22.163780920693917	30.67905905393526	26.635194231475403	20.521965793895422
47	21.57162807259617	28.20965983063861	27.220197625379726	22.99851447138549
48	22.620511422435378	24.760813201731445	28.620600442876697	23.99807493295648
49	20.67622152736822	23.330755449720144	31.682847986468893	24.310175036442743
50	19.82009525187221	31.008796332357818	26.99745178986725	22.173656625902723
51	19.101895022644573	31.102073063527214	25.704123871944095	24.091908041884118
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1626.0
1	2451.0
2	3276.0
3	16536.0
4	29796.0
5	22479.5
6	15163.0
7	15984.5
8	16806.0
9	17835.0
10	18864.0
11	18841.5
12	18819.0
13	18280.0
14	17741.0
15	16656.5
16	15572.0
17	15008.0
18	14444.0
19	13834.0
20	13224.0
21	13538.5
22	13853.0
23	15407.5
24	16962.0
25	17921.5
26	23715.0
27	28549.0
28	30412.0
29	32275.0
30	37095.0
31	41915.0
32	54749.5
33	67584.0
34	84513.0
35	101442.0
36	106200.0
37	110958.0
38	126580.5
39	142203.0
40	188782.0
41	235361.0
42	280403.0
43	325445.0
44	329229.0
45	333013.0
46	328998.0
47	324983.0
48	315630.0
49	306277.0
50	292831.5
51	279386.0
52	255030.0
53	230674.0
54	201473.0
55	172272.0
56	158571.5
57	144871.0
58	137522.5
59	130174.0
60	117857.5
61	105541.0
62	95546.5
63	85552.0
64	76628.5
65	67705.0
66	54677.0
67	41649.0
68	34830.5
69	28012.0
70	22956.0
71	17900.0
72	15243.5
73	12587.0
74	9282.5
75	4764.5
76	3551.0
77	2760.0
78	1969.0
79	1448.5
80	928.0
81	672.5
82	417.0
83	334.0
84	251.0
85	177.0
86	103.0
87	90.0
88	77.0
89	49.5
90	22.0
91	19.5
92	17.0
93	9.5
94	2.0
95	6.0
96	10.0
97	5.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3594680.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.19775043092686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.90276036423663	13.913454819172227
2	8.415864211725477	2.894678647476475
3	2.8992803273274266	1.4958329849601903
4	1.3203879551579847	0.9083081009923548
5	0.7678624347130041	0.6602753258739057
6	0.5015942937708473	0.5175776089108826
7	0.3433971254505163	0.4133960643536963
8	0.250334292727911	0.3444149352509762
9	0.20254409408964785	0.3134972503270739
>10	1.9488415977456226	7.5987001960068055
>50	0.7402774171268783	9.348675635167046
>100	1.6843199264095672	58.001664587533384
>500	0.019759790592242022	2.105082971928416
>1k	0.0022862567627387467	0.5347874051292228
>5k	1.6330405448133906E-4	0.15777941613359803
>10k+	3.266081089626781E-4	0.7918740507837184
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16914	0.4705286701458823	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	11282	0.31385269342472766	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	5618	0.15628651229038468	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0507694704396497	0.0
2	0.0	0.0	0.0	0.20519211723992123	0.0
3	0.0	0.0	0.0	0.28795330877852826	0.0
4	0.0	0.0	0.0	0.4393158779084647	0.0
5	0.0	0.0	0.0	0.7445447160804299	0.0
6	0.0	0.0	0.0	1.0591485194787853	0.0
7	0.0	0.0	0.0	1.2545762070615465	0.0
8	0.0	0.0	0.0	1.7888935871899585	0.0
9	0.0	0.0	0.0	2.0182046802497022	0.0
10	0.0	0.0	0.0	2.406027796632802	0.0
11	0.0	0.0	0.0	2.754153359965282	0.0
12	0.0	0.0	0.0	3.057156687104276	0.0
13	2.7818887912136825E-5	0.0	0.0	3.1790034161594356	0.0
14	2.7818887912136825E-5	0.0	0.0	3.223151991275997	0.0
15	2.7818887912136825E-5	0.0	0.0	3.3029643806959172	0.0
16	2.7818887912136825E-5	0.0	0.0	3.476999343474245	0.0
17	2.7818887912136825E-5	0.0	0.0	3.692094984810887	0.0
18	2.7818887912136825E-5	0.0	0.0	3.991342762081743	0.0
19	2.7818887912136825E-5	0.0	0.0	4.11471952997207	0.0
20	2.7818887912136825E-5	0.0	0.0	4.251059899629452	0.0
21	2.7818887912136825E-5	0.0	0.0	4.422006965849533	0.0
22	2.7818887912136825E-5	0.0	0.0	4.606557468258649	0.0
23	2.7818887912136825E-5	0.0	0.0	4.831695728131572	0.0
24	2.7818887912136825E-5	0.0	0.0	4.9923219869362505	0.0
25	2.7818887912136825E-5	0.0	0.0	5.116894967006799	0.0
26	2.7818887912136825E-5	0.0	0.0	5.239854451578443	0.0
27	2.7818887912136825E-5	0.0	0.0	5.3620628261764605	0.0
28	2.7818887912136825E-5	0.0	0.0	5.489501151701959	0.0
29	2.7818887912136825E-5	0.0	0.0	5.635522494352766	0.0
30	2.7818887912136825E-5	0.0	0.0	5.830783268608054	0.0
31	2.7818887912136825E-5	0.0	0.0	5.996027462806147	0.0
32	2.7818887912136825E-5	0.0	0.0	6.1477238585910285	0.0
33	0.003922463195611292	0.0	0.0	6.295469972292388	0.0
34	0.003922463195611292	0.0	0.0	6.457904458811354	0.0
35	0.003922463195611292	0.0	0.0	6.666657393704029	0.0
36	0.003922463195611292	0.0	0.0	6.835156397787842	0.0
37	0.003922463195611292	0.0	0.0	7.008356793928806	0.0
38	0.003922463195611292	0.0	0.0	7.173044610368656	0.0
39	0.003950282083523429	0.0	0.0	7.353839562909633	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTA	20	7.035291E-4	45.000004	28
CCGTACC	25	3.8924678E-5	45.000004	33
TGGTCGA	25	3.8924678E-5	45.000004	10
CGCGATA	30	2.1667965E-6	44.999996	23
ACGGGTA	145	0.0	43.448273	5
CGTTTTT	15430	0.0	43.118927	1
TGATGCG	105	0.0	42.857147	1
TCGATAG	205	0.0	41.707317	1
CGGTCTA	315	0.0	41.428574	31
TAATGCG	180	0.0	41.250004	1
TACGGGA	875	0.0	40.885715	4
GCTACGA	775	0.0	40.64516	10
GTATCGA	95	0.0	40.26316	22
TAAACGG	235	0.0	40.212765	2
GCGATAT	420	0.0	40.178574	9
CGTAGGT	45	1.9303116E-8	40.000004	21
CGTAGAC	45	1.9303116E-8	40.000004	34
GCGTAAG	85	0.0	39.705883	1
GCGTACA	40	3.4609548E-7	39.375004	9
ACCGCTC	120	0.0	39.374996	18
>>END_MODULE