SRR1546324_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1546324_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3185936
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	20511	0.6437982432792122	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12683	0.3980933703627443	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	4316	0.13547039237448585	No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	3213	0.10084948347989413	TruSeq Adapter, Index 27 (96% over 25bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	30	2.1667183E-6	45.000004	1
GTACCTC	25	3.8923674E-5	45.000004	18
GGTACGA	45	3.8562575E-10	45.000004	8
GCGACTA	25	3.8923674E-5	45.000004	9
TCACCGT	30	2.1667183E-6	45.000004	23
ATTACGC	20	7.03517E-4	45.000004	10
ATTACGA	25	3.8923674E-5	45.000004	18
CCAATCG	45	3.8562575E-10	45.000004	11
CGCGAGT	30	2.1667183E-6	45.000004	16
GCGCATT	40	6.8212103E-9	45.000004	43
CATTACG	25	3.8923674E-5	45.000004	17
CTAACGT	60	0.0	45.000004	31
CGGATAT	55	1.8189894E-12	45.000004	30
CTACGAT	20	7.03517E-4	45.000004	40
GTCCGAG	80	0.0	45.000004	1
ACCACGT	20	7.03517E-4	45.000004	42
CCGCGTA	35	1.2128294E-7	45.000004	11
CCGCGAA	25	3.8923674E-5	45.000004	45
ATCGGAT	20	7.03517E-4	45.000004	43
GCTAACC	20	7.03517E-4	45.000004	14