##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546324_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3185936
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.076409256180916	31.0	31.0	33.0	30.0	34.0
2	31.39832877998805	31.0	31.0	34.0	30.0	34.0
3	31.459188759598437	31.0	31.0	34.0	30.0	34.0
4	35.300028625810434	37.0	35.0	37.0	33.0	37.0
5	35.18981015312298	37.0	35.0	37.0	32.0	37.0
6	35.2322196679406	37.0	35.0	37.0	32.0	37.0
7	35.75494171885436	37.0	35.0	37.0	35.0	37.0
8	35.67565512929325	37.0	35.0	37.0	35.0	37.0
9	37.41637496798429	39.0	37.0	39.0	35.0	39.0
10	36.881491341947864	39.0	37.0	39.0	32.0	39.0
11	36.48860209370182	38.0	35.0	39.0	32.0	39.0
12	35.32275758207321	37.0	35.0	39.0	31.0	39.0
13	34.73702736024829	35.0	33.0	39.0	27.0	39.0
14	35.53575338613205	37.0	33.0	40.0	27.0	41.0
15	35.98129937324541	37.0	34.0	40.0	30.0	41.0
16	36.22070907890177	37.0	35.0	40.0	31.0	41.0
17	36.192497589405434	36.0	35.0	40.0	31.0	41.0
18	36.1177013599771	36.0	35.0	40.0	31.0	41.0
19	36.00094101074221	36.0	35.0	40.0	31.0	41.0
20	35.81177054404106	36.0	34.0	40.0	31.0	41.0
21	35.61245611964584	35.0	34.0	40.0	30.0	41.0
22	35.50075174140347	35.0	34.0	40.0	30.0	41.0
23	35.4971248637763	35.0	34.0	40.0	30.0	41.0
24	35.4404532922193	35.0	34.0	40.0	30.0	41.0
25	35.3705780655983	35.0	34.0	40.0	30.0	41.0
26	35.23082980951281	35.0	34.0	40.0	29.0	41.0
27	35.10122802215738	35.0	34.0	40.0	29.0	41.0
28	35.13445781710618	35.0	34.0	40.0	29.0	41.0
29	35.16093418072428	36.0	34.0	40.0	29.0	41.0
30	35.034313934743196	36.0	34.0	40.0	29.0	41.0
31	34.79040225541254	35.0	34.0	40.0	28.0	41.0
32	34.53166196684428	35.0	34.0	40.0	27.0	41.0
33	34.340461013655016	35.0	33.0	40.0	25.0	41.0
34	34.15930043792468	35.0	33.0	40.0	24.0	41.0
35	33.9465864976572	35.0	33.0	40.0	23.0	41.0
36	33.68288879625956	35.0	33.0	39.0	23.0	41.0
37	33.466020346924736	35.0	33.0	39.0	23.0	41.0
38	33.44927864213217	35.0	32.0	39.0	23.0	41.0
39	33.440829633740286	35.0	33.0	39.0	23.0	41.0
40	33.18618390325481	35.0	32.0	39.0	21.0	41.0
41	33.16400046956373	35.0	32.0	39.0	21.0	41.0
42	33.01075476720185	35.0	32.0	39.0	21.0	41.0
43	32.89486072538808	35.0	32.0	39.0	20.0	41.0
44	32.7262408912169	35.0	32.0	39.0	18.0	41.0
45	32.69835112820848	35.0	31.0	39.0	20.0	41.0
46	32.61756796118943	35.0	31.0	38.0	19.0	40.0
47	32.544020972172696	35.0	31.0	38.0	18.0	40.0
48	32.51808071474129	35.0	31.0	38.0	18.0	40.0
49	32.57030712481355	35.0	31.0	38.0	18.0	40.0
50	32.36105213664054	35.0	31.0	38.0	18.0	40.0
51	32.116619103459705	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	6.0
12	6.0
13	19.0
14	41.0
15	115.0
16	406.0
17	1153.0
18	2504.0
19	4717.0
20	8170.0
21	13258.0
22	19580.0
23	28356.0
24	39157.0
25	54374.0
26	70019.0
27	79799.0
28	85434.0
29	94143.0
30	108602.0
31	130742.0
32	160822.0
33	206957.0
34	366386.0
35	521763.0
36	198290.0
37	228212.0
38	315604.0
39	447222.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.880897795812597	24.075436543609165	29.767704059340804	14.275961601237439
2	28.97333781971766	26.91993812807288	30.788534358505633	13.318189693703827
3	26.981270182451876	25.233714676001025	33.812575017200594	13.972440124346502
4	23.86827607334234	28.204678311177627	33.1393662647335	14.78767935074653
5	21.93305201359977	32.3528784005705	31.864984105142096	13.849085480687624
6	21.064610211881217	40.479815037088	27.57522436106689	10.880350389963892
7	84.62511487989714	5.000696812490898	8.240717955414045	2.13347035219791
8	85.68213548545859	3.9874623972358516	7.223779762054228	3.1066223552513295
9	81.32916041000196	5.180486990322467	9.858264572797443	3.6320880268781295
10	56.08832066934176	21.585493242802116	13.155600112494412	9.170585975361714
11	51.38063037047825	18.018692152008075	20.024758814991888	10.575918662521783
12	44.79229965699248	20.940596421271486	22.441882071705145	11.825221850030886
13	20.492753150094668	46.521210721119324	22.455975261273295	10.530060867512718
14	13.703947599700683	47.57082377047122	26.316096745195132	12.409131884632963
15	10.5860255824348	21.984151596265587	55.33551207557213	12.094310745727473
16	11.971678024919521	17.077806961596213	51.44437929701036	19.506135716473903
17	13.233567780394836	17.746872504657972	29.15086806514632	39.86869164980087
18	19.783950462281727	21.9001574419574	37.78854942472165	20.527342671039218
19	29.301624389190494	23.63299199983929	25.762538858282152	21.302844752688063
20	31.45829043646828	22.275149281090393	24.552156728823178	21.71440355361815
21	20.019360087584936	29.962340737541492	28.120558605069277	21.897740569804288
22	21.28859462336971	25.487140984627437	24.816192164563255	28.408072227439597
23	16.918387563340882	33.311340843005006	24.57638822625439	25.19388336739972
24	18.92768718517886	23.331447963800905	41.11834010476042	16.622524746259813
25	14.042937460137303	26.020359479914223	38.439441344710005	21.497261715238473
26	15.210726141391415	35.278047016638126	28.532242957799532	20.978983884170933
27	16.974226726462803	34.84602327228168	28.93956438547416	19.240185615781357
28	12.338979816292605	29.319829400213944	40.71083034938555	17.630360434107903
29	12.750852496723098	23.40809733779963	40.612083858558364	23.228966306918906
30	16.895882403161895	30.787624107954457	32.55809281793483	19.75840067094882
31	28.263844597003835	27.261125145012326	23.59294097558771	20.882089282396134
32	29.19427760005223	26.678188136861507	25.40079273406622	18.726741529020043
33	26.986763073708953	27.601276359600448	24.942403111675816	20.46955745501479
34	16.752502247377222	25.875221598927283	30.923157276229023	26.449118877466464
35	17.432396633202927	24.40466475158321	33.072980750398	25.089957864815865
36	29.074532570648	22.483973312709356	28.807075848353513	19.63441826828913
37	18.190133135128892	31.12162328433465	31.170682650247837	19.517560930288617
38	18.00070685663491	32.63540133888439	25.72229950633032	23.64159229815037
39	18.067845681771384	31.542661246176944	28.46152590635845	21.92796716569322
40	22.2183998674173	25.08512412050964	26.522880560061466	26.17359545201159
41	15.690114302358868	22.740695356090015	26.913283882664306	34.65590645888681
42	21.414177811481462	25.09275139236946	24.140566539943052	29.352504256206025
43	21.22559900763857	26.69413321548204	25.872836114724212	26.207431662155173
44	19.234629948624203	30.76643724167717	27.980442796088813	22.018490013609814
45	15.357684523480696	37.496547325495555	21.785748364060044	25.360019786963704
46	21.32657404291863	32.88333475625373	26.460481315381102	19.329609885446537
47	21.07719677984743	26.035645411583914	27.95225641695251	24.934901391616155
48	22.1275631400003	21.9352491701026	31.21045118294906	24.726736506948036
49	19.81417705817066	20.478440244876232	34.31986706575399	25.387515631199122
50	17.61799358179198	32.32553949608529	27.150357069319657	22.90610985280307
51	16.454222558143037	33.22066105533821	24.098067255588308	26.227049130930443
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2098.0
1	2377.0
2	2656.0
3	11708.0
4	20760.0
5	16143.0
6	11526.0
7	12904.5
8	14283.0
9	15472.5
10	16662.0
11	17115.0
12	17568.0
13	17528.5
14	17489.0
15	16611.0
16	15733.0
17	14936.0
18	14139.0
19	14079.5
20	14020.0
21	14013.5
22	14007.0
23	13665.5
24	13324.0
25	14738.5
26	16839.5
27	17526.0
28	19333.5
29	21141.0
30	30951.5
31	40762.0
32	44925.0
33	49088.0
34	59739.0
35	70390.0
36	70379.0
37	70368.0
38	96355.5
39	122343.0
40	183335.5
41	244328.0
42	306281.5
43	368235.0
44	370061.5
45	371888.0
46	363314.0
47	354740.0
48	331864.5
49	308989.0
50	288148.5
51	267308.0
52	234616.0
53	201924.0
54	172215.5
55	142507.0
56	121834.5
57	101162.0
58	88044.0
59	74926.0
60	68556.5
61	62187.0
62	52320.5
63	42454.0
64	34805.5
65	27157.0
66	21439.5
67	15722.0
68	12400.0
69	9078.0
70	6846.5
71	4615.0
72	3862.5
73	3110.0
74	2378.5
75	1172.5
76	698.0
77	639.5
78	581.0
79	498.5
80	416.0
81	268.0
82	120.0
83	79.0
84	38.0
85	31.0
86	24.0
87	21.0
88	18.0
89	14.5
90	11.0
91	9.5
92	8.0
93	8.0
94	8.0
95	4.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3185936.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.39912493234666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.26756184687251	14.768528984354692
2	8.86544785002444	3.262329651476075
3	3.2851728419867507	1.8133291663219964
4	1.608467154275097	1.1837755248433448
5	0.9066551224836209	0.83408304345641
6	0.5776078394320131	0.6376487279767461
7	0.4110993131694257	0.5294707335824317
8	0.278680250384971	0.41019781944085837
9	0.2279182644674104	0.3774146960069559
>10	1.7304481210727438	6.758472751435711
>50	0.4654375702078999	6.246677501085114
>100	1.3433192468047184	57.607606376408036
>500	0.028226047733857073	3.360569724691424
>1k	0.003614310990310967	1.1587494451335583
>5k	0.0	0.0
>10k+	3.442200943153302E-4	1.0511458537867109
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	20511	0.6437982432792122	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12683	0.3980933703627443	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	4316	0.13547039237448585	No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	3213	0.10084948347989413	TruSeq Adapter, Index 27 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15351846364773178	0.0
2	0.0	0.0	0.0	0.6072312814821139	0.0
3	0.0	0.0	0.0	0.8204496261067391	0.0
4	0.0	0.0	0.0	1.1998043902953481	0.0
5	0.0	0.0	0.0	1.9432279870028777	0.0
6	0.0	0.0	0.0	2.6257589606319773	0.0
7	0.0	0.0	0.0	3.071781730706455	0.0
8	3.138795004042768E-5	0.0	0.0	4.282320799915629	0.0
9	3.138795004042768E-5	0.0	0.0	4.734275892547747	0.0
10	3.138795004042768E-5	0.0	0.0	5.502213478236851	0.0
11	3.138795004042768E-5	0.0	0.0	6.093813560598832	0.0
12	3.138795004042768E-5	0.0	0.0	6.659110540826934	0.0
13	3.138795004042768E-5	0.0	0.0	6.868468167596587	0.0
14	3.138795004042768E-5	0.0	0.0	6.938714399787064	0.0
15	3.138795004042768E-5	0.0	0.0	7.062477086796471	0.0
16	3.138795004042768E-5	0.0	0.0	7.3450941889604815	0.0
17	3.138795004042768E-5	0.0	0.0	7.6826088157452	0.0
18	3.138795004042768E-5	0.0	0.0	8.206379538069816	0.0
19	6.277590008085536E-5	0.0	0.0	8.394738626262424	0.0
20	6.277590008085536E-5	0.0	0.0	8.594962359570312	0.0
21	6.277590008085536E-5	0.0	0.0	8.84794923689616	0.0
22	6.277590008085536E-5	0.0	0.0	9.093842437512869	0.0
23	6.277590008085536E-5	0.0	0.0	9.400565485307927	0.0
24	6.277590008085536E-5	0.0	0.0	9.615541555134817	0.0
25	6.277590008085536E-5	0.0	0.0	9.773925151038815	0.0
26	6.277590008085536E-5	0.0	0.0	9.947908558112907	0.0
27	6.277590008085536E-5	0.0	0.0	10.113793874076567	0.0
28	6.277590008085536E-5	0.0	0.0	10.28432460664621	0.0
29	6.277590008085536E-5	0.0	0.0	10.4686346492836	0.0
30	6.277590008085536E-5	0.0	0.0	10.718419955705325	0.0
31	6.277590008085536E-5	0.0	0.0	10.94626508504879	0.0
32	6.277590008085536E-5	0.0	0.0	11.137166597194671	0.0
33	6.277590008085536E-5	0.0	0.0	11.319436423079434	0.0
34	6.277590008085536E-5	0.0	0.0	11.511719005027095	0.0
35	6.277590008085536E-5	0.0	0.0	11.778391028570567	0.0
36	9.416385012128303E-5	0.0	0.0	11.98109440993165	0.0
37	9.416385012128303E-5	0.0	0.0	12.188380431998635	0.0
38	9.416385012128303E-5	0.0	0.0	12.377932262292777	0.0
39	9.416385012128303E-5	0.0	0.0	12.563340883181583	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	30	2.1667183E-6	45.000004	1
GTACCTC	25	3.8923674E-5	45.000004	18
GGTACGA	45	3.8562575E-10	45.000004	8
GCGACTA	25	3.8923674E-5	45.000004	9
TCACCGT	30	2.1667183E-6	45.000004	23
ATTACGC	20	7.03517E-4	45.000004	10
ATTACGA	25	3.8923674E-5	45.000004	18
CCAATCG	45	3.8562575E-10	45.000004	11
CGCGAGT	30	2.1667183E-6	45.000004	16
GCGCATT	40	6.8212103E-9	45.000004	43
CATTACG	25	3.8923674E-5	45.000004	17
CTAACGT	60	0.0	45.000004	31
CGGATAT	55	1.8189894E-12	45.000004	30
CTACGAT	20	7.03517E-4	45.000004	40
GTCCGAG	80	0.0	45.000004	1
ACCACGT	20	7.03517E-4	45.000004	42
CCGCGTA	35	1.2128294E-7	45.000004	11
CCGCGAA	25	3.8923674E-5	45.000004	45
ATCGGAT	20	7.03517E-4	45.000004	43
GCTAACC	20	7.03517E-4	45.000004	14
>>END_MODULE