FastQCFastQC Report
Thu 26 May 2016
SRR1546323_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546323_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1560853
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC75620.48447867928626204No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66560.42643349501842903No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT32740.20975710076477413No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGA207.0340623E-445.00000442
CAACGTA207.0340623E-445.00000444
CTATCGT207.0340623E-445.00000413
CGACATA207.0340623E-445.00000441
ATCGTTA207.0340623E-445.00000415
ACGATAC207.0340623E-445.00000434
TATCGTT207.0340623E-445.00000414
TTCGTGA351.2123019E-745.015
TACGCAA253.8914495E-545.017
TGCGATG551.8189894E-1245.01
ACCGTTA253.8914495E-545.021
TTACCCG351.2123019E-745.01
TCGATAG1150.044.9999961
CACTCGA600.044.99999644
TGTTACG600.044.9999961
AACTACG302.1660035E-644.9999961
GCGCTAT302.1660035E-644.99999638
CGTTTTT63650.042.454831
CGAGGGT2300.042.0652164
AGCTACG3350.041.641799
Produced by FastQC (version 0.11.5)