##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546323_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1560853
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15021978366957	31.0	31.0	33.0	30.0	34.0
2	31.483242175912785	31.0	31.0	34.0	30.0	34.0
3	31.5544564414458	31.0	31.0	34.0	30.0	34.0
4	35.39446315572319	37.0	35.0	37.0	33.0	37.0
5	35.300326167806965	37.0	35.0	37.0	33.0	37.0
6	35.329612718173976	37.0	35.0	37.0	33.0	37.0
7	35.77460785865165	37.0	35.0	37.0	35.0	37.0
8	35.6922580153288	37.0	35.0	37.0	35.0	37.0
9	37.412575047105655	39.0	37.0	39.0	35.0	39.0
10	36.953350507703156	39.0	37.0	39.0	32.0	39.0
11	36.624479691553276	39.0	35.0	39.0	32.0	39.0
12	35.649656950398274	37.0	35.0	39.0	31.0	39.0
13	35.179085410349344	37.0	35.0	39.0	30.0	39.0
14	36.04923269519936	38.0	35.0	40.0	30.0	41.0
15	36.417653680391425	38.0	35.0	40.0	31.0	41.0
16	36.58897474650079	38.0	35.0	40.0	32.0	41.0
17	36.57107235594896	38.0	35.0	40.0	32.0	41.0
18	36.46659166494218	38.0	35.0	40.0	31.0	41.0
19	36.34508887127743	37.0	35.0	40.0	31.0	41.0
20	36.16404876051749	37.0	35.0	40.0	31.0	41.0
21	35.994075034612486	36.0	34.0	40.0	31.0	41.0
22	35.88046920497958	36.0	34.0	40.0	30.0	41.0
23	35.86690610839073	36.0	34.0	40.0	30.0	41.0
24	35.81297021564491	36.0	34.0	40.0	30.0	41.0
25	35.74034133899861	36.0	34.0	40.0	30.0	41.0
26	35.62221938901357	36.0	34.0	40.0	30.0	41.0
27	35.514209217652144	36.0	34.0	40.0	30.0	41.0
28	35.51432902393755	36.0	34.0	40.0	30.0	41.0
29	35.5006409956607	36.0	34.0	40.0	30.0	41.0
30	35.392386727001195	36.0	34.0	40.0	29.0	41.0
31	35.15577892344763	36.0	34.0	40.0	29.0	41.0
32	34.95185709352514	35.0	34.0	40.0	27.0	41.0
33	34.740626439517364	35.0	34.0	40.0	27.0	41.0
34	34.53499272513171	35.0	34.0	40.0	25.0	41.0
35	34.32648942597413	35.0	33.0	40.0	24.0	41.0
36	34.07475527804348	35.0	33.0	40.0	23.0	41.0
37	33.88515894834427	35.0	33.0	40.0	23.0	41.0
38	33.874898533045716	35.0	33.0	40.0	23.0	41.0
39	33.862382299934715	35.0	33.0	40.0	23.0	41.0
40	33.63718492388457	35.0	33.0	40.0	23.0	41.0
41	33.58783626645174	35.0	33.0	40.0	22.0	41.0
42	33.4742432503253	35.0	33.0	40.0	22.0	41.0
43	33.40550135086392	35.0	32.0	40.0	22.0	41.0
44	33.27479781888493	35.0	32.0	39.0	22.0	41.0
45	33.232347953330645	35.0	32.0	39.0	22.0	41.0
46	33.169595727464404	35.0	32.0	39.0	22.0	41.0
47	33.13608328266659	35.0	32.0	39.0	21.0	41.0
48	33.08416807988965	35.0	32.0	39.0	21.0	41.0
49	33.12742968107823	35.0	32.0	39.0	21.0	41.0
50	32.94490448491946	35.0	32.0	39.0	20.0	41.0
51	32.737978528407226	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	5.0
12	8.0
13	10.0
14	34.0
15	66.0
16	160.0
17	475.0
18	1062.0
19	2236.0
20	3873.0
21	5969.0
22	8793.0
23	12662.0
24	17966.0
25	24632.0
26	31205.0
27	35324.0
28	37770.0
29	41665.0
30	48659.0
31	58107.0
32	72376.0
33	94631.0
34	166975.0
35	229800.0
36	103481.0
37	123667.0
38	177738.0
39	261459.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.48361440827547	24.0123189051115	27.88994223030612	14.614124456306904
2	31.47881318740458	25.768666235705734	29.393158740765468	13.359361836124222
3	28.551439501349584	25.271374049958578	32.643624992231814	13.533561456460024
4	25.784042443458798	27.5442338259913	31.732905020524033	14.938818710025863
5	23.102816216517507	32.087006271570736	30.643949173945273	14.166228337966484
6	21.254275706937168	39.71962766512926	28.407415688729177	10.618680939204395
7	84.5368526055945	5.341117965625206	7.878768852672224	2.243260576108064
8	85.97670632660474	4.253956009950969	7.497502967928433	2.2718346955158495
9	81.22923811531258	6.05431773523836	9.528828147173373	3.187616002275679
10	52.679400302270615	22.51563728294721	13.98325146570497	10.821710949077204
11	47.70615810713757	19.39535625712351	20.46304168297719	12.435443952761727
12	42.69902418741547	21.236849338150357	23.90852950277829	12.155596971655882
13	21.588003482711056	42.75867105999092	22.795740534182272	12.857584923115759
14	14.430955381448477	44.57421679043446	26.71821113199001	14.276616696127054
15	13.22866407022314	22.19049455650212	51.286251812310326	13.29458956096442
16	13.746457866307718	17.649451934294902	48.761030026530364	19.843060172867016
17	15.400425280279437	18.018929393094673	29.298915400745617	37.28172992588027
18	21.51349294264098	22.72936657071486	34.98766379665478	20.769476689989382
19	29.315124486418643	24.337653834153507	25.167904985286892	21.179316694140958
20	31.49547074580374	22.611610446339277	25.3278175459188	20.565101261938185
21	21.587170604791098	27.79768498378771	28.369039236878805	22.246105174542382
22	21.8779090663887	25.24081383704936	25.816780952466377	27.064496144095568
23	19.30726340020489	30.706415017942113	25.15726977492435	24.829051806928646
24	20.4472810700303	22.93502334941215	38.998996061768786	17.618699518788763
25	16.97251438796607	25.90333618860969	35.48489191486963	21.639257508554618
26	16.61258299147966	34.36665720602773	27.12190065304036	21.898859149452253
27	17.504467108689926	33.05308059118956	29.788903887810065	19.65354841231045
28	14.054750831756738	28.2796650293141	39.57611639276728	18.089467746161876
29	15.011215021529894	24.16902808912819	38.65963034315211	22.160126546189808
30	18.608863230554064	29.42269387315782	32.815774451533876	19.152668444754248
31	27.305325998028003	27.513032937759036	24.956802466343724	20.22483859786924
32	29.22081707886649	27.233826631976232	25.399124709373655	18.146231579783617
33	26.883825702997015	28.78522192672853	25.70184379951219	18.629108570762266
34	19.190276086216958	27.16078964514916	29.28155309949111	24.36738116914277
35	19.933010988222467	26.651196493199553	30.63023872203212	22.785553796545862
36	28.9977979989147	25.345884590028657	26.34809299786719	19.308224413189453
37	20.280257013312593	31.637252194793486	29.260731151492163	18.821759640401755
38	20.274683138002107	31.683060480391173	24.98537658575151	23.056879795855213
39	20.04692306065978	31.688442153104745	27.487469992369558	20.777164793865918
40	23.293096787461728	26.004306619521504	26.123344094543178	24.579252498473593
41	16.89326285050546	24.407167106703834	27.493620475470788	31.20594956731992
42	22.436898285745038	27.210890455411242	23.69383920202607	26.658372056817655
43	22.431772883160683	28.116420957002358	25.832285295284052	23.61952086455291
44	20.56702328790732	31.35605979550925	27.50816380530389	20.568753111279538
45	17.25063154569969	36.73420879480643	22.50589901803693	23.509260641456947
46	21.788727061420904	32.82089985411823	26.298760997992765	19.091612086468103
47	21.96670666616267	28.02358710269321	27.42590109382498	22.583805137319146
48	23.701783576031822	22.570927563325952	30.092327720803947	23.634961139838282
49	20.439977371347588	23.01363421154971	32.09469437544727	24.45169404165543
50	18.518335807407873	32.813339885306306	26.681372300914948	21.986952006370874
51	17.83274914421794	33.53749520294352	24.353350379568095	24.27640527327045
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	749.0
1	1160.0
2	1571.0
3	6869.0
4	12167.0
5	9285.0
6	6403.0
7	6757.5
8	7112.0
9	7690.5
10	8269.0
11	8236.0
12	8203.0
13	8032.0
14	7861.0
15	7430.0
16	6999.0
17	7108.5
18	7218.0
19	7055.5
20	6893.0
21	6464.0
22	6035.0
23	6492.0
24	6949.0
25	7322.5
26	8871.0
27	10046.0
28	13176.5
29	16307.0
30	19679.0
31	23051.0
32	26022.5
33	28994.0
34	36052.5
35	43111.0
36	46155.5
37	49200.0
38	56950.0
39	64700.0
40	88220.0
41	111740.0
42	137212.5
43	162685.0
44	157658.5
45	152632.0
46	147993.5
47	143355.0
48	135736.0
49	128117.0
50	122808.5
51	117500.0
52	108493.5
53	99487.0
54	86258.5
55	73030.0
56	64870.0
57	56710.0
58	52873.5
59	49037.0
60	46429.0
61	43821.0
62	38512.5
63	33204.0
64	27558.0
65	21912.0
66	18252.0
67	14592.0
68	11368.0
69	8144.0
70	7109.0
71	6074.0
72	5011.0
73	3948.0
74	2990.0
75	1853.5
76	1675.0
77	1227.5
78	780.0
79	559.0
80	338.0
81	324.5
82	311.0
83	207.5
84	104.0
85	73.5
86	43.0
87	36.0
88	29.0
89	16.5
90	4.0
91	6.0
92	8.0
93	6.5
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1560853.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.087278187215794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.84016682378663	15.811933092409802
2	6.506850073399992	2.4839611494698235
3	1.9859728406622261	1.1372044824592529
4	0.9539088069775024	0.728300910560589
5	0.5577430273284306	0.5322898159798829
6	0.4231344185434314	0.4845890614394567
7	0.323431539664053	0.43213994404611095
8	0.24174903871453016	0.3691464922748991
9	0.21147061245695217	0.3632758567548074
>10	2.836889164106394	13.555599970155555
>50	1.6226062786964293	23.024325991466263
>100	1.4920284517780211	39.611175926442
>500	0.0030366929140665144	0.3395297012865499
>1k	3.3741032378516823E-4	0.21085361191429522
>5k	6.748206475703365E-4	0.9156739933406994
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7562	0.48447867928626204	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6656	0.42643349501842903	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3274	0.20975710076477413	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05561061804026388	0.0
2	0.0	0.0	0.0	0.2149465708814347	0.0
3	0.0	0.0	0.0	0.3021424823477932	0.0
4	0.0	0.0	0.0	0.44898526638959596	0.0
5	0.0	0.0	0.0	0.7488213175744288	0.0
6	0.0	0.0	0.0	1.04301942591647	0.0
7	0.0	0.0	0.0	1.2270854462271592	0.0
8	0.0	0.0	0.0	1.7085529514951119	0.0
9	0.0	0.0	0.0	1.9355442184497835	0.0
10	0.0	0.0	0.0	2.3223839785040616	0.0
11	0.0	0.0	0.0	2.6191447881382808	0.0
12	0.0	0.0	0.0	2.9099473172681862	0.0
13	0.0	0.0	0.0	3.018477716991927	0.0
14	6.406753230445148E-5	0.0	0.0	3.0569182363745977	0.0
15	6.406753230445148E-5	0.0	0.0	3.1248298206173164	0.0
16	6.406753230445148E-5	0.0	0.0	3.2757088591942995	0.0
17	6.406753230445148E-5	0.0	0.0	3.445744089930314	0.0
18	6.406753230445148E-5	0.0	0.0	3.707459959393998	0.0
19	6.406753230445148E-5	0.0	0.0	3.808366322773509	0.0
20	6.406753230445148E-5	0.0	0.0	3.9231112731307816	0.0
21	6.406753230445148E-5	0.0	0.0	4.059254779277741	0.0
22	6.406753230445148E-5	0.0	0.0	4.191874571147956	0.0
23	6.406753230445148E-5	0.0	0.0	4.355631183718134	0.0
24	6.406753230445148E-5	0.0	0.0	4.474796793804414	0.0
25	6.406753230445148E-5	0.0	0.0	4.56455540656295	0.0
26	6.406753230445148E-5	0.0	0.0	4.660848907616541	0.0
27	6.406753230445148E-5	0.0	0.0	4.757975286590089	0.0
28	6.406753230445148E-5	0.0	0.0	4.8531796395945035	0.0
29	6.406753230445148E-5	0.0	0.0	4.955367353620104	0.0
30	6.406753230445148E-5	0.0	0.0	5.105733851938652	0.0
31	6.406753230445148E-5	0.0	0.0	5.243991586651658	0.0
32	1.2813506460890295E-4	0.0	0.0	5.351625040923136	0.0
33	1.2813506460890295E-4	0.0	0.0	5.471110988670938	0.0
34	1.2813506460890295E-4	0.0	0.0	5.593223705243223	0.0
35	1.2813506460890295E-4	0.0	0.0	5.75883827625023	0.0
36	1.2813506460890295E-4	0.0	0.0	5.882168275936299	0.0
37	1.2813506460890295E-4	0.0	0.0	6.007804706785328	0.0
38	1.2813506460890295E-4	0.0	0.0	6.117808659752072	0.0
39	1.2813506460890295E-4	0.0	0.0	6.233706825690824	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCGA	20	7.0340623E-4	45.000004	42
CAACGTA	20	7.0340623E-4	45.000004	44
CTATCGT	20	7.0340623E-4	45.000004	13
CGACATA	20	7.0340623E-4	45.000004	41
ATCGTTA	20	7.0340623E-4	45.000004	15
ACGATAC	20	7.0340623E-4	45.000004	34
TATCGTT	20	7.0340623E-4	45.000004	14
TTCGTGA	35	1.2123019E-7	45.0	15
TACGCAA	25	3.8914495E-5	45.0	17
TGCGATG	55	1.8189894E-12	45.0	1
ACCGTTA	25	3.8914495E-5	45.0	21
TTACCCG	35	1.2123019E-7	45.0	1
TCGATAG	115	0.0	44.999996	1
CACTCGA	60	0.0	44.999996	44
TGTTACG	60	0.0	44.999996	1
AACTACG	30	2.1660035E-6	44.999996	1
GCGCTAT	30	2.1660035E-6	44.999996	38
CGTTTTT	6365	0.0	42.45483	1
CGAGGGT	230	0.0	42.065216	4
AGCTACG	335	0.0	41.64179	9
>>END_MODULE