FastQCFastQC Report
Thu 26 May 2016
SRR1546322_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546322_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1377266
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55300.40152011303553564No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC28950.21019904651679486No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT22120.16060804521421423No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAA207.0337753E-445.044
TCGCGCG406.8175723E-945.01
CGTTAGG2300.042.065222
GCGTAAG1400.041.7857131
CGTTTTT45200.041.3163721
TAACGGG3650.041.301373
CGATCTA556.184564E-1140.90909210
ATTCGGC2050.040.60975617
ACGGGAT3050.040.5737725
CGACGGT900.040.028
CGTACTC403.458881E-739.37543
TCGTACT403.458881E-739.37542
ACGTATG403.458881E-739.3751
CTCGTAG356.249191E-638.5714261
ACTAACG356.249191E-638.5714261
AGTACGG1350.038.3333322
GCTACGA2950.038.13559310
AGCTACG2950.038.1355939
CACGACG900.037.526
CGGCACT301.1400397E-437.49999615
Produced by FastQC (version 0.11.5)