##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546322_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1377266
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.117416679130972	31.0	31.0	33.0	30.0	34.0
2	31.450217314592823	31.0	31.0	34.0	30.0	34.0
3	31.55019146628175	31.0	31.0	34.0	30.0	34.0
4	35.40158328166091	37.0	35.0	37.0	33.0	37.0
5	35.310828118896424	37.0	35.0	37.0	33.0	37.0
6	35.341190445418675	37.0	35.0	37.0	33.0	37.0
7	35.76016470311472	37.0	35.0	37.0	35.0	37.0
8	35.65836446989906	37.0	35.0	37.0	35.0	37.0
9	37.37066913726179	39.0	37.0	39.0	35.0	39.0
10	36.86417075568554	39.0	37.0	39.0	32.0	39.0
11	36.60974132810946	39.0	35.0	39.0	32.0	39.0
12	35.99693232825032	37.0	35.0	39.0	32.0	39.0
13	35.74566568840006	37.0	35.0	39.0	31.0	39.0
14	36.66371565115236	38.0	35.0	41.0	31.0	41.0
15	36.90783987987796	38.0	35.0	41.0	31.0	41.0
16	36.96413546838446	38.0	35.0	41.0	32.0	41.0
17	36.89999607918877	38.0	35.0	41.0	32.0	41.0
18	36.82788364774851	38.0	35.0	40.0	31.0	41.0
19	36.75369318635616	38.0	35.0	40.0	31.0	41.0
20	36.60624817573366	38.0	35.0	40.0	31.0	41.0
21	36.473646339922716	38.0	35.0	40.0	31.0	41.0
22	36.35679672626784	38.0	35.0	40.0	30.0	41.0
23	36.28040189767263	38.0	35.0	40.0	30.0	41.0
24	36.289962868465494	38.0	35.0	40.0	30.0	41.0
25	36.225636151622126	38.0	35.0	40.0	30.0	41.0
26	36.108785811891096	38.0	35.0	40.0	30.0	41.0
27	35.977987549246116	38.0	34.0	40.0	30.0	41.0
28	35.96124641136861	38.0	35.0	40.0	30.0	41.0
29	35.88345751655817	38.0	35.0	40.0	30.0	41.0
30	35.73704062976941	37.0	34.0	40.0	29.0	41.0
31	35.54777798914662	37.0	34.0	40.0	29.0	41.0
32	35.40961005354085	37.0	34.0	40.0	28.0	41.0
33	35.23439408218891	37.0	34.0	40.0	27.0	41.0
34	35.03582024097015	37.0	34.0	40.0	26.0	41.0
35	34.87275297582312	37.0	34.0	40.0	25.0	41.0
36	34.650581659606786	37.0	33.0	40.0	24.0	41.0
37	34.49619173057347	37.0	33.0	40.0	24.0	41.0
38	34.402293384139305	37.0	33.0	40.0	24.0	41.0
39	34.349997749163926	36.0	33.0	40.0	23.0	41.0
40	34.16311300794472	36.0	33.0	40.0	23.0	41.0
41	34.04948644633644	36.0	33.0	40.0	23.0	41.0
42	33.92597290574225	36.0	33.0	40.0	23.0	41.0
43	33.893335782630224	36.0	33.0	40.0	23.0	41.0
44	33.72020292376346	35.0	33.0	40.0	23.0	41.0
45	33.6968189151551	35.0	33.0	40.0	23.0	41.0
46	33.58254832399842	35.0	32.0	40.0	23.0	41.0
47	33.498536956550154	35.0	32.0	40.0	22.0	41.0
48	33.419580531284446	35.0	32.0	39.0	22.0	41.0
49	33.41213389425136	35.0	32.0	39.0	22.0	41.0
50	33.258857766037934	35.0	32.0	39.0	22.0	41.0
51	33.05775717980405	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	4.0
12	13.0
13	13.0
14	29.0
15	69.0
16	190.0
17	431.0
18	954.0
19	1704.0
20	2920.0
21	4674.0
22	7186.0
23	9905.0
24	13662.0
25	18798.0
26	24296.0
27	28008.0
28	31219.0
29	35219.0
30	41140.0
31	49600.0
32	60923.0
33	77718.0
34	124154.0
35	163402.0
36	107854.0
37	134478.0
38	189499.0
39	249167.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.66178646681178	23.021188354319357	28.6347009219715	13.68232425689736
2	31.808016752029022	24.94057066681382	29.869466029074992	13.38194655208217
3	29.66522080701912	25.645009751202746	30.928738529811962	13.761030911966172
4	26.36041258551362	27.69247189722247	30.247969528035977	15.699145989227933
5	22.787827478497256	31.983218927934036	29.723452114551584	15.505501479017125
6	21.07494122413535	40.60907624235261	27.45998231278489	10.85600022072715
7	86.33009164533213	4.447506872310796	7.136747730648982	2.085653751708094
8	87.09319768294577	3.890969500445084	6.688613528541327	2.3272192880678095
9	81.71892720796127	5.408831699904012	8.879112676854	3.9931284152807085
10	45.700394840212425	24.63402131469157	15.855615400365652	13.809968444730359
11	39.18015837173066	23.58665646287645	22.16129636540799	15.071888799984897
12	34.56848568105217	22.761470914115357	26.440716608120724	16.229326796711746
13	23.880644697538457	33.52613075469807	25.910317977790783	16.682906569972687
14	18.946376371739373	35.75416804016072	28.220547083860342	17.07890850423956
15	18.53643377532009	23.252951862603158	40.99520354092819	17.215410821148566
16	19.467481227301047	20.396858704128324	39.03196622874594	21.103693839824697
17	20.21323404483956	20.50380972157884	28.48614574090989	30.79681049267171
18	23.392213268896494	23.44826634796764	31.807072853029116	21.352447530106748
19	28.485201841909984	24.84639858966968	25.525933261984253	21.142466306436084
20	30.271058749725903	23.809561842084246	25.421813941533443	20.497565466656408
21	24.929243878814987	26.84768229230955	27.53622030893088	20.686853519944588
22	24.500278087166894	24.50804710201225	25.797630958725478	25.194043852095383
23	21.86164473674657	28.885051979791847	25.353925821155826	23.899377462305758
24	22.145032259563514	24.85424021213041	33.831445777358915	19.169281750947167
25	20.864742177618556	26.08319671000373	31.08019801548866	21.971863096889056
26	20.13423695930924	29.858284456306915	26.767741307779325	23.23973727660452
27	20.418132735433822	28.77664881003379	28.528185550213248	22.277032904319135
28	18.78010493252574	26.890157747305167	33.71476533944786	20.614971980721226
29	18.975709848351734	24.629519642538188	33.75368302129	22.641087487820073
30	21.816700622828126	26.839913277464195	30.766678332290205	20.576707767417478
31	27.336404151413017	25.624026150358752	25.71899691127204	21.320572786956188
32	28.003232491036588	25.18395139355796	26.298260466750794	20.514555648654653
33	26.815880156774362	25.75101686965336	25.742303955808097	21.690799017764178
34	21.16657203474129	26.362518206359553	29.404777290661354	23.0661324682378
35	22.626057711437007	25.035614035342483	29.33427529613016	23.00405295709035
36	27.786498759135853	25.368955597538896	25.768733127805376	21.07581251551988
37	22.643338323896764	29.369489989588065	27.493309208242998	20.49386247827217
38	22.241309957553586	30.21159311273204	25.282044281932464	22.26505264778191
39	22.472419997299	28.949963187939005	26.15486042638096	22.42275638838104
40	24.662919145611667	26.060470526390688	25.68813867473676	23.588471653260882
41	20.530892362114507	24.463393418555313	27.1307067770496	27.875007442280577
42	22.79399912580431	25.90378329240684	25.28748985308575	26.014727728703097
43	22.773451170652585	26.506063461960146	26.106213324078283	24.61427204330899
44	21.58776881154403	28.216698880245357	27.745838494524662	22.449693813685954
45	20.89451129992318	30.808500318747434	24.783447787137707	23.51354059419168
46	23.137941399845783	29.43570813481201	26.71756944555373	20.708781019788482
47	22.62373426774494	26.88173526392142	27.675046069531955	22.819484398801684
48	23.792063406778357	24.30547185511005	29.166116058916725	22.73634867919487
49	22.253144998860062	24.32623763310791	29.758812023240246	23.661805344791784
50	21.2894241199594	29.614322868639753	27.252760178498566	21.843492832902285
51	20.780807774242593	29.78030387739188	25.325173205466484	24.113715142899046
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	409.0
1	845.0
2	1281.0
3	5788.5
4	10296.0
5	7669.0
6	5042.0
7	5140.5
8	5239.0
9	5397.0
10	5555.0
11	5510.5
12	5466.0
13	5425.5
14	5385.0
15	5178.0
16	4971.0
17	4782.0
18	4593.0
19	4563.5
20	4534.0
21	4862.5
22	5191.0
23	5847.0
24	6503.0
25	7108.5
26	8951.5
27	10189.0
28	11952.5
29	13716.0
30	16636.0
31	19556.0
32	21615.5
33	23675.0
34	30674.0
35	37673.0
36	41288.0
37	44903.0
38	49884.0
39	54865.0
40	67648.5
41	80432.0
42	89998.5
43	99565.0
44	103828.0
45	108091.0
46	104909.0
47	101727.0
48	103998.0
49	106269.0
50	106351.5
51	106434.0
52	101911.0
53	97388.0
54	87645.5
55	77903.0
56	74296.5
57	70690.0
58	65189.0
59	59688.0
60	55924.5
61	52161.0
62	47868.0
63	43575.0
64	37771.0
65	31967.0
66	28030.5
67	24094.0
68	19719.5
69	15345.0
70	12639.5
71	9934.0
72	8312.5
73	6691.0
74	5334.0
75	3093.5
76	2210.0
77	1666.5
78	1123.0
79	826.5
80	530.0
81	443.0
82	356.0
83	263.5
84	171.0
85	136.5
86	102.0
87	69.0
88	36.0
89	28.0
90	20.0
91	21.0
92	22.0
93	12.5
94	3.0
95	4.5
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1377266.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.793926326117926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.55605345968813	17.33838767897142
2	5.790536086130005	2.523970336996891
3	2.00133878475045	1.3085109008536107
4	1.192901097060993	1.039919944947701
5	0.8414304551196852	0.9169036673715146
6	0.6181902422644598	0.8083675557261978
7	0.5106688911121187	0.7790636132956498
8	0.4180016090710742	0.728791701783499
9	0.3658176385183311	0.7175342396396652
>10	6.341740705797583	35.65913178138162
>50	2.1851846608093473	32.12275284731162
>100	0.17579247007688018	5.0704032929986536
>500	0.001339371200584895	0.21002318154952912
>1k	6.696856002924475E-4	0.37268533293031453
>5k	3.3484280014622376E-4	0.4035539242421459
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5530	0.40152011303553564	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2895	0.21019904651679486	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2212	0.16060804521421423	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.036521630534697	0.0
2	0.0	0.0	0.0	0.14710302875406783	0.0
3	0.0	0.0	0.0	0.2079482104401038	0.0
4	0.0	0.0	0.0	0.2997968438921748	0.0
5	0.0	0.0	0.0	0.5244448058690188	0.0
6	0.0	0.0	0.0	0.754247908537639	0.0
7	0.0	0.0	0.0	0.9083938759832886	0.0
8	0.0	0.0	0.0	1.3408448331694822	0.0
9	0.0	0.0	0.0	1.5508260568401455	0.0
10	0.0	0.0	0.0	1.9038442828037576	0.0
11	0.0	0.0	0.0	2.1572448604699455	0.0
12	0.0	0.0	0.0	2.399391257752678	0.0
13	0.0	0.0	0.0	2.490804245512486	0.0
14	0.0	0.0	0.0	2.5269628379702977	0.0
15	0.0	0.0	0.0	2.5942700974248982	0.0
16	0.0	0.0	0.0	2.7316437057184304	0.0
17	0.0	0.0	0.0	2.8782384811648587	0.0
18	0.0	0.0	0.0	3.089454034296933	0.0
19	0.0	0.0	0.0	3.18166570582589	0.0
20	0.0	0.0	0.0	3.2856398110459417	0.0
21	0.0	0.0	0.0	3.406967136341128	0.0
22	0.0	0.0	0.0	3.542452946634855	0.0
23	0.0	0.0	0.0	3.698051066388047	0.0
24	0.0	0.0	0.0	3.814876719529851	0.0
25	0.0	0.0	0.0	3.9097022652123847	0.0
26	0.0	0.0	0.0	3.9997357082800273	0.0
27	0.0	0.0	0.0	4.096449051962366	0.0
28	0.0	0.0	0.0	4.193452826106213	0.0
29	0.0	0.0	0.0	4.308971542171229	0.0
30	0.0	0.0	0.0	4.453896342463983	0.0
31	0.0	0.0	0.0	4.573916730682381	0.0
32	0.0	0.0	0.0	4.692702789439368	0.0
33	0.0	0.0	0.0	4.809383227350417	0.0
34	0.0	0.0	0.0	4.935430047645117	0.0
35	7.260761537713121E-5	0.0	0.0	5.089866445552276	0.0
36	7.260761537713121E-5	0.0	0.0	5.214751544000941	0.0
37	7.260761537713121E-5	0.0	0.0	5.342686162295446	0.0
38	7.260761537713121E-5	0.0	0.0	5.476284174589368	0.0
39	7.260761537713121E-5	0.0	0.0	5.636819612188205	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTAA	20	7.0337753E-4	45.0	44
TCGCGCG	40	6.8175723E-9	45.0	1
CGTTAGG	230	0.0	42.06522	2
GCGTAAG	140	0.0	41.785713	1
CGTTTTT	4520	0.0	41.316372	1
TAACGGG	365	0.0	41.30137	3
CGATCTA	55	6.184564E-11	40.909092	10
ATTCGGC	205	0.0	40.609756	17
ACGGGAT	305	0.0	40.573772	5
CGACGGT	90	0.0	40.0	28
CGTACTC	40	3.458881E-7	39.375	43
TCGTACT	40	3.458881E-7	39.375	42
ACGTATG	40	3.458881E-7	39.375	1
CTCGTAG	35	6.249191E-6	38.571426	1
ACTAACG	35	6.249191E-6	38.571426	1
AGTACGG	135	0.0	38.333332	2
GCTACGA	295	0.0	38.135593	10
AGCTACG	295	0.0	38.135593	9
CACGACG	90	0.0	37.5	26
CGGCACT	30	1.1400397E-4	37.499996	15
>>END_MODULE