FastQCFastQC Report
Thu 26 May 2016
SRR1546321_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546321_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1988413
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC115510.5809155341470812No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87880.4419604981460089No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCATA207.0345297E-445.00000439
AGTCCGC207.0345297E-445.00000420
CGTTGAT406.8193913E-945.00000425
GACCGAA207.0345297E-445.0000049
TACGATC207.0345297E-445.00000445
GAACGAT207.0345297E-445.0000049
CGTAATT207.0345297E-445.00000433
TTACGCA207.0345297E-445.00000442
ACGTACG207.0345297E-445.00000431
ACGTAAC207.0345297E-445.00000432
CGGTATG207.0345297E-445.00000429
CGTCAGT406.8193913E-945.00000427
CCGTTCA207.0345297E-445.00000418
TCGCGAT207.0345297E-445.00000442
AATCGGT207.0345297E-445.0000043
CTTACGC207.0345297E-445.00000441
TCGCCTA253.8918377E-545.010
CTTCGAA253.8918377E-545.034
AACGTCG453.8562575E-1045.020
CTCGTTA253.8918377E-545.033