##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546321_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1988413
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23438440605649	31.0	31.0	33.0	30.0	34.0
2	31.56695766925684	31.0	31.0	34.0	30.0	34.0
3	31.615627638724952	31.0	31.0	34.0	30.0	34.0
4	35.46075287176256	37.0	35.0	37.0	33.0	37.0
5	35.378505370866115	37.0	35.0	37.0	33.0	37.0
6	35.41748369176826	37.0	35.0	37.0	33.0	37.0
7	35.852807741651255	37.0	35.0	37.0	35.0	37.0
8	35.77860635592304	37.0	35.0	37.0	35.0	37.0
9	37.54448095038606	39.0	37.0	39.0	35.0	39.0
10	37.08539574022097	39.0	37.0	39.0	33.0	39.0
11	36.711112832193315	39.0	35.0	39.0	32.0	39.0
12	35.5690628657125	37.0	35.0	39.0	31.0	39.0
13	35.024571354140214	37.0	35.0	39.0	30.0	39.0
14	35.88097844864221	37.0	35.0	40.0	30.0	41.0
15	36.30473950834158	37.0	35.0	40.0	31.0	41.0
16	36.515287317071454	37.0	35.0	40.0	32.0	41.0
17	36.47690696047552	37.0	35.0	40.0	32.0	41.0
18	36.386758183536315	37.0	35.0	40.0	31.0	41.0
19	36.269116627179564	37.0	35.0	40.0	31.0	41.0
20	36.080422930246385	36.0	35.0	40.0	31.0	41.0
21	35.887643562982134	36.0	34.0	40.0	31.0	41.0
22	35.760516552647765	35.0	34.0	40.0	30.0	41.0
23	35.7335664170371	35.0	34.0	40.0	31.0	41.0
24	35.7197277426772	35.0	34.0	40.0	31.0	41.0
25	35.663760496436105	35.0	34.0	40.0	31.0	41.0
26	35.53564073459588	35.0	34.0	40.0	30.0	41.0
27	35.4091348225947	35.0	34.0	40.0	30.0	41.0
28	35.43493378890603	36.0	34.0	40.0	30.0	41.0
29	35.44270782780036	36.0	34.0	40.0	30.0	41.0
30	35.33382602105297	36.0	34.0	40.0	30.0	41.0
31	35.057072147486465	35.0	34.0	40.0	29.0	41.0
32	34.81221858839185	35.0	34.0	40.0	29.0	41.0
33	34.59847576936984	35.0	34.0	40.0	27.0	41.0
34	34.375493421135346	35.0	34.0	40.0	25.0	41.0
35	34.1733674040554	35.0	33.0	40.0	24.0	41.0
36	33.912583049899595	35.0	33.0	40.0	23.0	41.0
37	33.68562366067814	35.0	33.0	39.0	23.0	41.0
38	33.67615832324572	35.0	33.0	39.0	23.0	41.0
39	33.66839736010577	35.0	33.0	39.0	23.0	41.0
40	33.434799008053155	35.0	33.0	39.0	23.0	41.0
41	33.421287227552824	35.0	33.0	39.0	23.0	41.0
42	33.33485598816745	35.0	33.0	39.0	22.0	41.0
43	33.24056420874336	35.0	32.0	39.0	23.0	41.0
44	33.032095947874005	35.0	32.0	39.0	21.0	41.0
45	32.97917384366326	35.0	32.0	39.0	22.0	41.0
46	32.93739831715041	35.0	32.0	39.0	22.0	41.0
47	32.87667451379568	35.0	32.0	39.0	21.0	40.0
48	32.860268465354025	35.0	32.0	38.0	21.0	40.0
49	32.90850140287757	35.0	32.0	38.0	21.0	40.0
50	32.71876365724827	35.0	32.0	38.0	20.0	40.0
51	32.50491874675935	35.0	32.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	1.0
12	6.0
13	10.0
14	25.0
15	62.0
16	186.0
17	486.0
18	1157.0
19	2245.0
20	4057.0
21	6539.0
22	10002.0
23	14978.0
24	21585.0
25	30594.0
26	39971.0
27	45578.0
28	48628.0
29	53411.0
30	62201.0
31	75427.0
32	94957.0
33	125557.0
34	230233.0
35	336900.0
36	128328.0
37	150484.0
38	212230.0
39	292514.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.858723514682314	23.493811396324606	29.30990694589102	15.337558143102061
2	30.342438919882337	25.696824553048085	30.14187696419205	13.818859562877531
3	26.829184882617447	25.45401785242804	33.77683609994503	13.939961165009482
4	24.77297221452485	27.89727285025797	32.330909121998296	14.998845813218884
5	23.09107816132765	31.53801549275729	31.37542351614076	13.995482829774296
6	21.183074140030264	39.45161291944883	28.28682974814588	11.078483192375025
7	85.45181509072813	4.684087259538134	7.935423878238574	1.9286737714951572
8	87.03855788510737	3.4450086576581427	7.320611965421671	2.1958214918128176
9	83.16763167410393	4.65456622945032	9.362843634597038	2.8149584618487205
10	56.842014209321704	20.1156902514719	12.684739035602766	10.357556503603629
11	51.32052546427729	17.56933795946818	19.459237090081384	11.650899486173143
12	44.503732373505905	20.392845953028875	22.78575929648418	12.317662376981039
13	20.168194434456023	44.99558190375943	23.633923133674944	11.202300528109603
14	13.81383042657637	46.347413741511446	27.54669175870405	12.292064073208131
15	11.354180444404658	21.151843203600055	54.45337563172238	13.040600720272902
16	13.499609990479843	15.795008381055645	51.546836597829525	19.158545030634983
17	13.932920374187857	16.381808004675086	29.48512205462346	40.2001495665136
18	20.904007366678854	20.882532954672897	37.07846408165708	21.134995596991168
19	29.58022302207841	22.285561399970728	26.143311273865137	21.99090430408572
20	31.52383332838802	21.607281787033177	25.048619175191472	21.820265709387336
21	21.881470298172463	28.001275388965972	28.26922777109182	21.848026541769745
22	22.69900669528916	24.313359447961766	25.246465397279138	27.74116845946994
23	17.784333536342803	31.120848636576003	25.383408778759748	25.71140904832145
24	19.896570782830327	22.521981097488297	40.55555862891663	17.025889490764744
25	15.72037599834642	23.97846926166747	38.39222535760931	21.908929382376797
26	15.832274281047248	34.756763308226205	28.09159867693482	21.31936373379172
27	17.168867835806747	34.20028937650277	28.92326694705778	19.707575840632703
28	13.779883756543535	28.309812901042186	40.7031637793557	17.20713956305858
29	14.148318281966574	24.14644241412624	39.71946471884865	21.985774585058536
30	17.577233703461	29.529780784977767	32.894876466810466	19.998109044750763
31	29.096671566721806	26.47085892115974	24.450101663990328	19.98236784812813
32	30.27243334257018	27.076668679997567	25.309631349221718	17.34126662821054
33	28.345972391047535	27.221608388197023	25.06712639677974	19.365292823975704
34	18.5247732739627	27.555341873142048	29.998898619150044	23.920986233745204
35	19.530499951468837	25.75863263818935	31.587653068049747	23.12321434229207
36	31.32281875043062	23.199657214069713	26.3875261326495	19.08999790285016
37	20.90511377666511	30.84525196727239	29.206910234443246	19.04272402161925
38	21.310110122997585	32.28519427302074	22.952676330319708	23.45201927366196
39	20.49730111400398	30.606921197960386	26.766119513400888	22.12965817463475
40	23.28213504940875	24.957994139044555	24.756024025189937	27.003846786356757
41	16.65881283214302	23.854098720939763	26.095081856737007	33.39200659018021
42	22.109793086245162	26.47548572655681	23.556373851911047	27.858347335286986
43	22.633175301106963	27.023611292020323	25.40166454353296	24.941548863339758
44	20.4809061296622	30.879751842298354	27.480508324980775	21.158833703058672
45	16.807071770301242	37.10667753630659	22.405556592116426	23.68069410127574
46	23.056125664034585	31.97841695864994	26.289608848865903	18.675848528449574
47	22.41189330385589	26.21039995212262	27.273307909372953	24.104398834648535
48	23.883871207842635	22.779472876107732	30.09193764072152	23.244718275328115
49	21.246541840150915	21.83852147416055	32.247425459399025	24.66751122628951
50	19.16628990053877	32.441751286075885	25.78775133737307	22.604207476012277
51	17.990528124690393	34.19385208203728	22.728980347644075	25.086639445628244
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1068.0
1	1466.5
2	1865.0
3	8792.5
4	15720.0
5	12107.0
6	8494.0
7	9231.0
8	9968.0
9	10676.0
10	11384.0
11	11513.5
12	11643.0
13	11396.0
14	11149.0
15	10603.5
16	10058.0
17	9440.5
18	8823.0
19	8401.5
20	7980.0
21	8529.0
22	9078.0
23	8316.5
24	7555.0
25	8375.0
26	10264.0
27	11333.0
28	12696.5
29	14060.0
30	17319.0
31	20578.0
32	24856.5
33	29135.0
34	34245.0
35	39355.0
36	41161.0
37	42967.0
38	53909.5
39	64852.0
40	100275.5
41	135699.0
42	178144.5
43	220590.0
44	219641.0
45	218692.0
46	207578.0
47	196464.0
48	185754.0
49	175044.0
50	166271.5
51	157499.0
52	143637.5
53	129776.0
54	115398.5
55	101021.0
56	89137.5
57	77254.0
58	69598.0
59	61942.0
60	57506.5
61	53071.0
62	45989.0
63	38907.0
64	33317.5
65	27728.0
66	23159.0
67	18590.0
68	15162.5
69	11735.0
70	9582.0
71	7429.0
72	6051.5
73	4674.0
74	3600.0
75	2182.5
76	1839.0
77	1429.0
78	1019.0
79	672.0
80	325.0
81	265.0
82	205.0
83	125.5
84	46.0
85	34.0
86	22.0
87	17.0
88	12.0
89	6.5
90	1.0
91	6.0
92	11.0
93	10.5
94	10.0
95	16.0
96	22.0
97	11.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1988413.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.249210714044555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.82851236341673	14.933057645367917
2	7.917240792758487	2.8896679100175753
3	2.5941391628274104	1.4202297661197762
4	1.1482940696038857	0.8382184175155621
5	0.6720253143247744	0.6131965783142416
6	0.4613978401674293	0.505208784493228
7	0.33217086254427136	0.42432992445454365
8	0.24709058373203202	0.3607366502385697
9	0.1826757381436193	0.30003192339538814
>10	1.9640896503270648	8.381205735255117
>50	0.9030566711586635	12.145743958750122
>100	1.738223111610182	54.88268192411461
>500	0.00942126347752952	1.0195485907962325
>1k	0.0011083839385328848	0.2576430944403732
>5k	2.770959846332212E-4	0.4443900910582924
>10k+	2.770959846332212E-4	0.5841090056684496
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	11551	0.5809155341470812	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8788	0.4419604981460089	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09097707568799841	0.0
2	0.0	0.0	0.0	0.3515366274511382	0.0
3	0.0	0.0	0.0	0.48772563848657197	0.0
4	0.0	0.0	0.0	0.7024194671831254	0.0
5	0.0	0.0	0.0	1.1087736803169161	0.0
6	0.0	0.0	0.0	1.523778007888703	0.0
7	0.0	0.0	0.0	1.766383543056699	0.0
8	0.0	0.0	0.0	2.393315674359401	0.0
9	0.0	0.0	0.0	2.6655931137042455	0.0
10	0.0	0.0	0.0	3.141299116431043	0.0
11	0.0	0.0	0.0	3.573754546967858	0.0
12	0.0	0.0	0.0	3.9637137757598646	0.0
13	0.0	0.0	0.0	4.114738738883723	0.0
14	0.0	0.0	0.0	4.170562151826608	0.0
15	0.0	0.0	0.0	4.26008077798727	0.0
16	0.0	0.0	0.0	4.485687832457342	0.0
17	0.0	0.0	0.0	4.736692025248276	0.0
18	0.0	0.0	0.0	5.141185457950637	0.0
19	0.0	0.0	0.0	5.286225748876114	0.0
20	0.0	0.0	0.0	5.4335794425001245	0.0
21	0.0	0.0	0.0	5.626044488745547	0.0
22	0.0	0.0	0.0	5.81066408236116	0.0
23	0.0	0.0	0.0	6.032801032783431	0.0
24	0.0	0.0	0.0	6.196901750290307	0.0
25	0.0	0.0	0.0	6.315187036093608	0.0
26	0.0	0.0	0.0	6.439104954554209	0.0
27	0.0	0.0	0.0	6.562922290288788	0.0
28	0.0	0.0	0.0	6.690058855982133	0.0
29	0.0	0.0	0.0	6.821269022079417	0.0
30	0.0	0.0	0.0	7.007497939311401	0.0
31	0.0	0.0	0.0	7.174917886777043	0.0
32	0.0	0.0	0.0	7.312615638702825	0.0
33	0.0	0.0	0.0	7.449760185635479	0.0
34	0.0	0.0	0.0	7.6071218604988	0.0
35	0.0	0.0	0.0	7.812159747497125	0.0
36	0.0	0.0	0.0	7.968364721011178	0.0
37	0.0	0.0	0.0	8.119842306402141	0.0
38	0.0	0.0	0.0	8.269157363183604	0.0
39	0.0	0.0	0.0	8.415656103636417	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCATA	20	7.0345297E-4	45.000004	39
AGTCCGC	20	7.0345297E-4	45.000004	20
CGTTGAT	40	6.8193913E-9	45.000004	25
GACCGAA	20	7.0345297E-4	45.000004	9
TACGATC	20	7.0345297E-4	45.000004	45
GAACGAT	20	7.0345297E-4	45.000004	9
CGTAATT	20	7.0345297E-4	45.000004	33
TTACGCA	20	7.0345297E-4	45.000004	42
ACGTACG	20	7.0345297E-4	45.000004	31
ACGTAAC	20	7.0345297E-4	45.000004	32
CGGTATG	20	7.0345297E-4	45.000004	29
CGTCAGT	40	6.8193913E-9	45.000004	27
CCGTTCA	20	7.0345297E-4	45.000004	18
TCGCGAT	20	7.0345297E-4	45.000004	42
AATCGGT	20	7.0345297E-4	45.000004	3
CTTACGC	20	7.0345297E-4	45.000004	41
TCGCCTA	25	3.8918377E-5	45.0	10
CTTCGAA	25	3.8918377E-5	45.0	34
AACGTCG	45	3.8562575E-10	45.0	20
CTCGTTA	25	3.8918377E-5	45.0	33
>>END_MODULE