FastQCFastQC Report
Thu 26 May 2016
SRR1546320_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546320_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3492769
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117200.335550389962806No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC79110.22649651322489403No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT35480.10158129552798939No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGC253.892446E-545.034
CGTTTTT103750.042.809641
TCGTTAG700.041.7857131
AACGTAG1950.040.3846171
TCGATAG1200.039.3750041
GAACGTA403.4609184E-739.37545
TAGCCGT1100.038.86363644
TCACGAC4000.038.812525
TAATACG700.038.5714261
ATCCGCG356.2519503E-638.5714261
TAGGGCG15050.038.1229255
AGGGCGA40050.037.9775246
CGGGTAT4350.037.758626
ACGGGAT12250.037.6530655
GGGCGAT75800.037.5494737
GTCGTAA301.1403983E-437.50000439
GTATCCG601.5643309E-1037.5000041
CTCACGA4150.037.40963724
CACGACG4150.037.40963726
CGACGGT4050.037.2222228
Produced by FastQC (version 0.11.5)