##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546320_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3492769
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.10834956448594	31.0	31.0	33.0	30.0	34.0
2	31.442338728956884	31.0	31.0	34.0	30.0	34.0
3	31.561653805333247	31.0	31.0	34.0	30.0	34.0
4	35.40077600322266	37.0	35.0	37.0	33.0	37.0
5	35.29410590852129	37.0	35.0	37.0	33.0	37.0
6	35.32518697915608	37.0	35.0	37.0	33.0	37.0
7	35.782156793077355	37.0	35.0	37.0	35.0	37.0
8	35.69228597711443	37.0	35.0	37.0	35.0	37.0
9	37.436991681957785	39.0	37.0	39.0	35.0	39.0
10	36.76892030363302	39.0	37.0	39.0	32.0	39.0
11	36.56715889313035	39.0	35.0	39.0	32.0	39.0
12	35.963458505271895	37.0	35.0	39.0	31.0	39.0
13	35.65281013430891	37.0	35.0	39.0	30.0	39.0
14	36.58388630911463	38.0	35.0	41.0	31.0	41.0
15	36.83362226359659	38.0	35.0	41.0	31.0	41.0
16	36.89729295009203	38.0	35.0	41.0	32.0	41.0
17	36.83852181464047	38.0	35.0	41.0	32.0	41.0
18	36.78777296752233	38.0	35.0	40.0	31.0	41.0
19	36.70893437269971	38.0	35.0	40.0	31.0	41.0
20	36.605460309571	38.0	35.0	40.0	31.0	41.0
21	36.46811398062683	38.0	35.0	40.0	31.0	41.0
22	36.35234165213903	38.0	35.0	40.0	31.0	41.0
23	36.27664698123466	38.0	35.0	40.0	30.0	41.0
24	36.27513729078562	38.0	35.0	40.0	30.0	41.0
25	36.21653421683484	38.0	35.0	40.0	30.0	41.0
26	36.116247882410775	38.0	35.0	40.0	30.0	41.0
27	36.00696524734387	38.0	34.0	40.0	30.0	41.0
28	35.986705390479585	38.0	35.0	40.0	30.0	41.0
29	35.94134310056004	38.0	35.0	40.0	30.0	41.0
30	35.804730859670364	38.0	34.0	40.0	30.0	41.0
31	35.613456830383	37.0	34.0	40.0	29.0	41.0
32	35.46564029857113	37.0	34.0	40.0	29.0	41.0
33	35.28822776427528	37.0	34.0	40.0	27.0	41.0
34	35.127410372687116	37.0	34.0	40.0	27.0	41.0
35	34.96856934999137	37.0	34.0	40.0	26.0	41.0
36	34.771392840465545	37.0	33.0	40.0	25.0	41.0
37	34.58090243013494	37.0	33.0	40.0	24.0	41.0
38	34.531594846381196	37.0	33.0	40.0	24.0	41.0
39	34.49107713679319	37.0	33.0	40.0	24.0	41.0
40	34.29924252076218	36.0	33.0	40.0	23.0	41.0
41	34.2224936719262	36.0	33.0	40.0	23.0	41.0
42	34.10841570112424	36.0	33.0	40.0	23.0	41.0
43	34.05826666464344	36.0	33.0	40.0	23.0	41.0
44	33.88825857077866	36.0	33.0	40.0	23.0	41.0
45	33.8577163276472	36.0	33.0	40.0	23.0	41.0
46	33.755970406287965	35.0	33.0	40.0	23.0	41.0
47	33.687907788920484	35.0	32.0	40.0	23.0	41.0
48	33.619133129044606	35.0	32.0	40.0	23.0	41.0
49	33.61560670058627	36.0	32.0	40.0	23.0	41.0
50	33.46541640744063	35.0	32.0	39.0	22.0	41.0
51	33.25127370289876	35.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	9.0
12	7.0
13	25.0
14	47.0
15	136.0
16	364.0
17	984.0
18	2123.0
19	4147.0
20	7213.0
21	11531.0
22	17157.0
23	24666.0
24	34216.0
25	47335.0
26	60536.0
27	70039.0
28	78123.0
29	88144.0
30	103593.0
31	124452.0
32	153626.0
33	195250.0
34	316047.0
35	421942.0
36	261522.0
37	328043.0
38	474557.0
39	666842.0
40	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.28027132627437	23.257163585682306	29.633966632204995	13.828598455838334
2	29.55726531013073	25.476749249664092	32.064416513087465	12.90156892711771
3	28.569424430874186	25.410984808900906	32.177106473402624	13.842484286822291
4	25.120069492142193	27.8611611589544	32.03255067827274	14.986218670630667
5	22.555800283385473	32.46152264864926	30.39556867345078	14.587108394514495
6	20.452197096343905	40.96277194397912	28.075861873487767	10.50916908618921
7	87.65930412231671	4.108201830696505	6.580710032641724	1.6517840143450653
8	89.16195144883615	3.074237088109749	5.856499528024899	1.907311935029199
9	84.23162253215143	4.4281771854938015	8.27077313157555	3.069427150779224
10	43.5670380720855	23.354937014156963	19.891381308068183	13.186643605689355
11	40.15329957406287	21.84224035428624	22.182543420420874	15.821916651230012
12	35.689448686701006	22.241436522140457	25.211114734469987	16.858000056688546
13	23.102100367931573	34.06159983669117	26.17513497170869	16.661164823668557
14	19.326185041152165	36.66200083658553	27.218404652583665	16.79340946967864
15	18.546803410131048	23.027460447570395	42.36899147925328	16.05674466304528
16	18.679477514831355	20.471207801031216	40.863710139433785	19.985604544703644
17	19.634479119575328	20.168009965732058	28.783752947876025	31.413757966816586
18	22.679913844860625	22.8604010170727	33.70512049322472	20.75456464484196
19	27.531565929496054	24.367972803240065	26.90948070141484	21.190980565849042
20	29.53542017808793	23.584411107634086	25.719880129490384	21.1602885847876
21	23.74173614115334	27.214396371474898	28.730786376081557	20.313081111290213
22	24.03053279504027	23.5509133297965	27.04541869216086	25.373135183002372
23	20.67394093339697	29.683383012160263	25.83958458174589	23.803091472696877
24	21.26115411583188	25.07735266775444	34.458534188776866	19.202959027636812
25	19.11686687553629	25.63390249970725	33.597641298350965	21.651589326405496
26	19.67645154890003	30.425144061917635	27.383316789630236	22.515087599552103
27	20.240817529014947	29.61006009844911	28.77447664016716	21.374645732368787
28	17.676176122726698	27.469781139262288	35.01579978521339	19.83824295279762
29	18.868467969109894	24.430960077806464	34.54474086319479	22.155831089888853
30	20.896200120878305	27.628251395955473	30.62873611166384	20.84681237150238
31	26.525630524091344	26.749636176912933	25.794147852320037	20.93058544667569
32	27.97319261594454	26.005269744434862	26.336124719384536	19.685412920236065
33	26.33678322270955	26.484717426202536	25.85699197398969	21.32150737709823
34	20.263378425541454	27.341945602471853	29.210176796690533	23.18449917529616
35	21.241169971446723	25.934924410975935	30.22083624768772	22.60306936988962
36	28.48124224648123	25.1828849832325	26.266752825623453	20.069119944662816
37	21.52074757878348	29.771336151918437	28.41207076677559	20.295845502522496
38	21.592638963527218	30.312912190872055	25.468618165129158	22.625830680471566
39	20.894367763800012	29.25950728490776	27.394081887465216	22.45204306382701
40	22.993533211042585	25.60034746071097	26.828141225486142	24.577978102760305
41	19.404833242622114	24.647664932894216	27.803212866353316	28.144288958130353
42	22.505439094311704	26.782160515052674	25.703159871150945	25.00924051948468
43	22.61286102802676	27.430070525706107	26.53917278812312	23.417895658144012
44	20.84140119200554	29.548590244588173	27.72170160694853	21.888306956457757
45	20.12134212139423	31.42017121659062	25.080559292641453	23.377927369373698
46	22.347398296308747	29.703968398711737	27.508260637906485	20.44037266707303
47	22.16619535961296	27.362702772499414	27.96832541745532	22.502776450432307
48	23.31425296090294	25.020950426438166	28.769409027622494	22.8953875850364
49	21.20446556872212	24.398034911555847	30.735241866839747	23.662257652882282
50	20.592286521095442	29.24272976540962	27.627478370313067	22.53750534318187
51	20.030898121232752	30.261176733989565	25.97778438826043	23.73014075651725
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1106.0
1	2127.0
2	3148.0
3	12879.5
4	22611.0
5	17072.0
6	11533.0
7	11824.0
8	12115.0
9	12827.5
10	13540.0
11	13397.0
12	13254.0
13	12967.0
14	12680.0
15	12361.5
16	12043.0
17	11452.0
18	10861.0
19	10972.5
20	11084.0
21	11379.5
22	11675.0
23	12910.0
24	14145.0
25	15785.5
26	22090.5
27	26755.0
28	32123.5
29	37492.0
30	42070.0
31	46648.0
32	55430.0
33	64212.0
34	80369.5
35	96527.0
36	100800.5
37	105074.0
38	124297.5
39	143521.0
40	177186.5
41	210852.0
42	251200.0
43	291548.0
44	301774.5
45	312001.0
46	313225.5
47	314450.0
48	312412.5
49	310375.0
50	299604.0
51	288833.0
52	265907.5
53	242982.0
54	215974.0
55	188966.0
56	177134.5
57	165303.0
58	152381.0
59	139459.0
60	125516.5
61	111574.0
62	95636.5
63	79699.0
64	68449.5
65	57200.0
66	45773.0
67	34346.0
68	29287.5
69	24229.0
70	19009.0
71	13789.0
72	11314.0
73	8839.0
74	7124.0
75	4109.0
76	2809.0
77	1932.5
78	1056.0
79	956.0
80	856.0
81	646.5
82	437.0
83	305.5
84	174.0
85	130.5
86	87.0
87	53.5
88	20.0
89	17.0
90	14.0
91	10.0
92	6.0
93	5.0
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3492769.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.677793636091614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.70620923505545	14.26707712008541
2	8.01656498794042	2.83430363054256
3	2.5866689203209288	1.3717979813497583
4	1.206063590415064	0.8528217305344489
5	0.6759808622484056	0.5974925092387293
6	0.4535280696892414	0.48104253744848313
7	0.349439428878048	0.4324122678413891
8	0.2844024633152655	0.40220864448667026
9	0.2330085653717635	0.3707169600675418
>10	2.4833988922478163	10.67166801246248
>50	1.2586222857751694	16.350602020324672
>100	1.737776116164429	49.53399506385218
>500	0.006211571332695099	0.6935460092321293
>1k	0.0017980864384117392	0.5730474109029773
>5k	1.6346240349197628E-4	0.2286005782690461
>10k+	1.6346240349197628E-4	0.33866752336154976
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11720	0.335550389962806	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7911	0.22649651322489403	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3548	0.10158129552798939	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06046778358374115	0.0
2	0.0	0.0	0.0	0.22632472974880388	0.0
3	0.0	0.0	0.0	0.31221646779389073	0.0
4	0.0	0.0	0.0	0.4562855430748498	0.0
5	0.0	0.0	0.0	0.7879994354049753	0.0
6	0.0	0.0	0.0	1.094060328638968	0.0
7	0.0	0.0	0.0	1.3062701827690293	0.0
8	0.0	0.0	0.0	1.8652536139664546	0.0
9	0.0	0.0	0.0	2.103631817620919	0.0
10	0.0	0.0	0.0	2.571226439538372	0.0
11	0.0	0.0	0.0	2.9087237088968667	0.0
12	0.0	0.0	0.0	3.25498193553596	0.0
13	0.0	0.0	0.0	3.3808419623513606	0.0
14	0.0	0.0	0.0	3.42630732235656	0.0
15	0.0	0.0	0.0	3.506186638738491	0.0
16	0.0	0.0	0.0	3.6838107530157305	0.0
17	0.0	0.0	0.0	3.8762368768160735	0.0
18	0.0	0.0	0.0	4.162055950450775	0.0
19	0.0	0.0	0.0	4.280042567945375	0.0
20	0.0	0.0	0.0	4.413747373502227	0.0
21	0.0	0.0	0.0	4.583870275990196	0.0
22	0.0	0.0	0.0	4.756054580191247	0.0
23	0.0	0.0	0.0	4.95827236212873	0.0
24	0.0	0.0	0.0	5.103257615948836	0.0
25	0.0	0.0	0.0	5.225567450925039	0.0
26	0.0	0.0	0.0	5.348965247916481	0.0
27	0.0	0.0	0.0	5.479263014530878	0.0
28	2.863057934836229E-5	0.0	0.0	5.60981845635941	0.0
29	2.863057934836229E-5	0.0	0.0	5.748390460405483	0.0
30	5.726115869672458E-5	0.0	0.0	5.928877632617559	0.0
31	5.726115869672458E-5	0.0	0.0	6.081736295758466	0.0
32	5.726115869672458E-5	0.0	0.0	6.221310370081731	0.0
33	5.726115869672458E-5	0.0	0.0	6.370704733121486	0.0
34	5.726115869672458E-5	0.0	0.0	6.523906963214572	0.0
35	5.726115869672458E-5	0.0	0.0	6.714672513412711	0.0
36	5.726115869672458E-5	0.0	0.0	6.865154838467703	0.0
37	5.726115869672458E-5	0.0	0.0	7.025915541508757	0.0
38	8.589173804508686E-5	0.0	0.0	7.187764206565049	0.0
39	1.1452231739344915E-4	0.0	0.0	7.381421445277372	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGC	25	3.892446E-5	45.0	34
CGTTTTT	10375	0.0	42.80964	1
TCGTTAG	70	0.0	41.785713	1
AACGTAG	195	0.0	40.384617	1
TCGATAG	120	0.0	39.375004	1
GAACGTA	40	3.4609184E-7	39.375	45
TAGCCGT	110	0.0	38.863636	44
TCACGAC	400	0.0	38.8125	25
TAATACG	70	0.0	38.571426	1
ATCCGCG	35	6.2519503E-6	38.571426	1
TAGGGCG	1505	0.0	38.122925	5
AGGGCGA	4005	0.0	37.977524	6
CGGGTAT	435	0.0	37.75862	6
ACGGGAT	1225	0.0	37.653065	5
GGGCGAT	7580	0.0	37.549473	7
GTCGTAA	30	1.1403983E-4	37.500004	39
GTATCCG	60	1.5643309E-10	37.500004	1
CTCACGA	415	0.0	37.409637	24
CACGACG	415	0.0	37.409637	26
CGACGGT	405	0.0	37.22222	28
>>END_MODULE