FastQCFastQC Report
Thu 26 May 2016
SRR1546319_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546319_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2129139
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC97130.4561937947686835No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78680.3695390484134667No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTACG351.2125747E-745.0000041
TTCGATC351.2125747E-745.00000427
CCTTCGT207.034643E-445.012
CGAAATA207.034643E-445.09
GCGCAAC207.034643E-445.038
TACGATC207.034643E-445.035
TACGACA253.891931E-545.021
GTCACGA253.891931E-545.010
ACTACGC207.034643E-445.039
GTCGTTA207.034643E-445.022
TATTACG207.034643E-445.01
CTACCGT207.034643E-445.022
TTACGTC253.891931E-545.035
TCGACCA253.891931E-545.028
GCGAATC302.1663782E-644.9999969
CGAATCC302.1663782E-644.99999610
CGGTAAT302.1663782E-644.9999966
TAAGCGT302.1663782E-644.99999627
GTACGAC302.1663782E-644.9999969
CGTTTTT74250.042.8484841
Produced by FastQC (version 0.11.5)