##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546319_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2129139
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.122894747595154	31.0	31.0	33.0	30.0	34.0
2	31.45311132810023	31.0	31.0	34.0	30.0	34.0
3	31.546562248871492	31.0	31.0	34.0	30.0	34.0
4	35.38149411569653	37.0	35.0	37.0	33.0	37.0
5	35.270660581577815	37.0	35.0	37.0	33.0	37.0
6	35.30590910222395	37.0	35.0	37.0	32.0	37.0
7	35.75130181730737	37.0	35.0	37.0	35.0	37.0
8	35.673699556487385	37.0	35.0	37.0	35.0	37.0
9	37.4245706832668	39.0	37.0	39.0	35.0	39.0
10	36.92664922299578	39.0	37.0	39.0	32.0	39.0
11	36.567463655496425	39.0	35.0	39.0	32.0	39.0
12	35.55136137189728	37.0	35.0	39.0	31.0	39.0
13	35.05481746377291	37.0	34.0	39.0	30.0	39.0
14	35.88616525271483	38.0	35.0	40.0	30.0	41.0
15	36.295724234068324	38.0	35.0	40.0	31.0	41.0
16	36.47111907677235	38.0	35.0	40.0	31.0	41.0
17	36.43752615493869	37.0	35.0	40.0	31.0	41.0
18	36.36921967048652	37.0	35.0	40.0	31.0	41.0
19	36.23818078575424	37.0	35.0	40.0	31.0	41.0
20	36.06759821693182	36.0	34.0	40.0	31.0	41.0
21	35.897554363524414	36.0	34.0	40.0	30.0	41.0
22	35.76021340081601	36.0	34.0	40.0	30.0	41.0
23	35.73583077478737	36.0	34.0	40.0	30.0	41.0
24	35.6964937470029	36.0	34.0	40.0	30.0	41.0
25	35.643266597436806	36.0	34.0	40.0	30.0	41.0
26	35.50147923644253	35.0	34.0	40.0	30.0	41.0
27	35.3951672483572	36.0	34.0	40.0	29.0	41.0
28	35.37722807200469	36.0	34.0	40.0	29.0	41.0
29	35.37005146211685	36.0	34.0	40.0	29.0	41.0
30	35.251670276106914	36.0	34.0	40.0	29.0	41.0
31	35.006604547659876	35.0	34.0	40.0	29.0	41.0
32	34.799601623003475	35.0	34.0	40.0	27.0	41.0
33	34.627741542473274	35.0	33.0	40.0	26.0	41.0
34	34.44670874001181	35.0	34.0	40.0	25.0	41.0
35	34.27554800320693	35.0	33.0	40.0	24.0	41.0
36	34.02758016268548	35.0	33.0	40.0	23.0	41.0
37	33.839186638354754	35.0	33.0	40.0	23.0	41.0
38	33.82663179811182	35.0	33.0	40.0	23.0	41.0
39	33.8215607341747	35.0	33.0	40.0	23.0	41.0
40	33.609773246368604	35.0	33.0	40.0	23.0	41.0
41	33.56146780459143	35.0	33.0	39.0	23.0	41.0
42	33.429833373960086	35.0	33.0	39.0	23.0	41.0
43	33.363207850685185	35.0	32.0	39.0	23.0	41.0
44	33.192988809091375	35.0	32.0	39.0	22.0	41.0
45	33.15611803644572	35.0	32.0	39.0	23.0	41.0
46	33.09883056014662	35.0	32.0	39.0	22.0	41.0
47	33.024541845318694	35.0	32.0	39.0	22.0	41.0
48	32.97919299773289	35.0	32.0	39.0	22.0	41.0
49	33.018842828016396	35.0	32.0	39.0	22.0	40.0
50	32.83965255438935	35.0	32.0	38.0	21.0	40.0
51	32.616590086415215	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	6.0
12	2.0
13	8.0
14	32.0
15	86.0
16	243.0
17	671.0
18	1506.0
19	3048.0
20	5143.0
21	8151.0
22	11934.0
23	17092.0
24	23990.0
25	32435.0
26	40964.0
27	48121.0
28	52393.0
29	58757.0
30	69225.0
31	82537.0
32	102033.0
33	132438.0
34	233458.0
35	328202.0
36	143297.0
37	172020.0
38	238379.0
39	322922.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.56987918590566	23.230798928580988	30.058582365923503	14.140739519589843
2	29.952483139898334	26.0364870494599	30.28242871883893	13.728601091802837
3	28.652380140516893	24.360081704388488	32.306110592121975	14.681427562972637
4	25.266363539440125	27.644695813659887	31.371272613013996	15.717668033885998
5	22.607495330272002	31.565200768949325	31.314254259585685	14.51304964119299
6	21.147703367417535	40.832045253973554	27.227109174177922	10.793142204430993
7	85.85071242413012	4.931007322678322	7.207279562301945	2.011000690889604
8	87.73043939357646	3.409547239517946	6.392161338456531	2.467852028449059
9	83.19132757419784	5.197077316229707	8.413260007918694	3.1983351016537673
10	55.427146841986364	20.52026664299513	12.440145993286487	11.612440521732024
11	48.54145267171378	18.933381052153006	19.302403459802296	13.22276281633092
12	42.227444990674634	20.5186227860182	23.80873207432676	13.44520014898041
13	21.282405704841253	41.80163906630802	24.002143589497916	12.91381163935281
14	14.951114041873264	43.663612380403535	28.55745914193484	12.827814435788362
15	13.042549124317388	21.174286883101573	52.0798313308807	13.70333266170034
16	14.761178110024757	16.52348672397622	48.25931984713069	20.45601531886833
17	15.560562274233858	16.753110059981992	28.95306506526817	38.733262600515985
18	22.76882815072196	20.75331859498135	34.851176931144465	21.62667632315222
19	30.263500879933158	22.894137019706086	24.94459027804197	21.897771822318788
20	32.35735196245994	21.6753344896693	23.80478681758213	22.16252673028863
21	22.963132045394875	27.37505630210146	27.538596587634718	22.123215064868944
22	23.05283966899296	23.998245300095487	24.872777211821305	28.076137819090253
23	19.315413413591127	30.591567765185836	24.28700051992848	25.806018301294557
24	20.8919661891497	23.381094423614428	37.69039973435272	18.03653965288316
25	17.052949572573702	24.41090036864667	36.27658879951004	22.259561259269592
26	16.639589993889548	33.29420953728244	28.272179505424493	21.794020963403515
27	18.800980114496987	31.80473421415887	29.0851372315288	20.309148439815345
28	15.597290735832653	26.62099562311338	39.13445763757087	18.647256003483097
29	15.582542990382498	23.79562818585353	38.10568497406698	22.51614384969699
30	19.351437365056956	29.17357673688754	31.51428817000675	19.960697728048757
31	29.766257628083466	26.09881271255658	23.0267258267309	21.10820383262906
32	30.147914250784	26.305234181516568	24.921623247707174	18.62522831999226
33	28.51340377495316	26.290392501382016	24.498917167925626	20.697286555739197
34	19.71120720629325	26.934455664942497	28.613209377123805	24.74112775164045
35	20.45507597202437	25.009827916354922	30.83438892434923	23.700707187271476
36	30.880651756414213	22.414130782443046	27.04562736392504	19.659590097217702
37	21.08875935295911	31.188428749837378	28.074024288691348	19.648787608512173
38	20.70113787779943	32.18601509812182	23.404531127371207	23.708315896707543
39	21.198803835728903	30.631161234658705	26.29114397885718	21.87889095075521
40	24.095326796418647	25.031198057054986	24.587309705942168	26.2861654405842
41	18.185567029677255	23.131556934516723	26.403489861394675	32.27938617441134
42	22.82603437351906	25.684466819686268	23.481134862496063	28.00836394429861
43	23.083556310790417	26.649222995774345	24.995315007615755	25.27190568581948
44	21.20481565553024	29.365203493055176	27.561704519996113	21.868276331418475
45	17.72091911331294	35.49942018816057	22.996337956328823	23.78332274219767
46	23.142265488537856	31.074908683744933	26.139862169637585	19.642963658079626
47	22.289244619538696	26.91721865035585	26.93168459175282	23.861852138352642
48	24.006887291059908	22.970224114066767	29.970095893222563	23.052792701650763
49	21.363894043554694	22.25293886401968	32.47646114227394	23.90670595015168
50	19.46237422732851	31.76255754086511	26.46196420243112	22.313104029375257
51	18.393914159667357	33.71334609905694	24.094387449574686	23.79835229170101
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1098.0
1	1564.5
2	2031.0
3	8087.5
4	14144.0
5	10628.0
6	7112.0
7	7709.0
8	8306.0
9	8878.0
10	9450.0
11	9711.0
12	9972.0
13	9794.5
14	9617.0
15	8856.5
16	8096.0
17	8033.5
18	7971.0
19	7590.0
20	7209.0
21	7479.0
22	7749.0
23	8087.5
24	8426.0
25	8923.5
26	10653.5
27	11886.0
28	13952.0
29	16018.0
30	18166.0
31	20314.0
32	23204.0
33	26094.0
34	33815.0
35	41536.0
36	44385.5
37	47235.0
38	65443.0
39	83651.0
40	115480.5
41	147310.0
42	177718.5
43	208127.0
44	215550.0
45	222973.0
46	213197.0
47	203421.0
48	190309.0
49	177197.0
50	171681.0
51	166165.0
52	151247.0
53	136329.0
54	123312.5
55	110296.0
56	103120.5
57	95945.0
58	89144.0
59	82343.0
60	72660.5
61	62978.0
62	58326.5
63	53675.0
64	45735.0
65	37795.0
66	30587.0
67	23379.0
68	19975.5
69	16572.0
70	14095.0
71	11618.0
72	9344.5
73	7071.0
74	5631.5
75	3248.0
76	2304.0
77	1648.0
78	992.0
79	836.0
80	680.0
81	468.0
82	256.0
83	167.5
84	79.0
85	73.0
86	67.0
87	43.5
88	20.0
89	17.5
90	15.0
91	8.0
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2129139.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.95595496730603
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.7855692601071	14.685379986099715
2	7.770180451860664	2.790420205629034
3	2.499352493218542	1.3463478244696858
4	1.1168669166205871	0.8021764823725279
5	0.6681247199145224	0.5998408691664558
6	0.4486682986054689	0.48337606590105686
7	0.3142353602547629	0.3949677184508797
8	0.23651920433739948	0.3397542545588319
9	0.19117084902190146	0.308938964048903
>10	2.1313063216548787	9.025418438518725
>50	1.0368592413514928	13.72226698602567
>100	1.7914123760892902	53.49794441870063
>500	0.00841903304938829	1.0071819729442524
>1k	7.892843483801521E-4	0.1654386148804302
>5k	5.261895655867681E-4	0.8305471982332505
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	9713	0.4561937947686835	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7868	0.3695390484134667	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06237263043887693	0.0
2	0.0	0.0	0.0	0.2704379563757932	0.0
3	0.0	0.0	0.0	0.36958601575566463	0.0
4	0.0	0.0	0.0	0.5525707809588759	0.0
5	0.0	0.0	0.0	0.929107963359837	0.0
6	0.0	0.0	0.0	1.2886899352273384	0.0
7	0.0	0.0	0.0	1.5135695696711207	0.0
8	0.0	0.0	0.0	2.115033353858062	0.0
9	0.0	0.0	0.0	2.3803988372764766	0.0
10	0.0	0.0	0.0	2.8130150262617892	0.0
11	0.0	0.0	0.0	3.1553130162004455	0.0
12	0.0	0.0	0.0	3.4833799014531226	0.0
13	0.0	0.0	0.0	3.6072327828291155	0.0
14	0.0	0.0	0.0	3.6520396272859594	0.0
15	0.0	0.0	0.0	3.72944180722818	0.0
16	4.69673421979495E-5	0.0	0.0	3.912849278511173	0.0
17	4.69673421979495E-5	0.0	0.0	4.1282415098309695	0.0
18	4.69673421979495E-5	0.0	0.0	4.4555569176084795	0.0
19	4.69673421979495E-5	0.0	0.0	4.574337326027093	0.0
20	4.69673421979495E-5	0.0	0.0	4.720170923551727	0.0
21	4.69673421979495E-5	0.0	0.0	4.88868974735797	0.0
22	4.69673421979495E-5	0.0	0.0	5.045419768272527	0.0
23	4.69673421979495E-5	0.0	0.0	5.247989915172283	0.0
24	4.69673421979495E-5	0.0	0.0	5.377713714323019	0.0
25	4.69673421979495E-5	0.0	0.0	5.484846221876543	0.0
26	4.69673421979495E-5	0.0	0.0	5.5993056348129455	0.0
27	4.69673421979495E-5	0.0	0.0	5.706719946419656	0.0
28	4.69673421979495E-5	0.0	0.0	5.834283247829287	0.0
29	4.69673421979495E-5	0.0	0.0	5.955928664121976	0.0
30	4.69673421979495E-5	0.0	0.0	6.1213946106853525	0.0
31	4.69673421979495E-5	0.0	0.0	6.278077664257712	0.0
32	4.69673421979495E-5	0.0	0.0	6.414799597395943	0.0
33	4.69673421979495E-5	0.0	0.0	6.555372852594406	0.0
34	4.69673421979495E-5	0.0	0.0	6.6952415976599	0.0
35	4.69673421979495E-5	0.0	0.0	6.888606145488858	0.0
36	4.69673421979495E-5	0.0	0.0	7.0409682035790055	0.0
37	4.69673421979495E-5	0.0	0.0	7.189525906951119	0.0
38	4.69673421979495E-5	0.0	0.0	7.3234297995574735	0.0
39	4.69673421979495E-5	0.0	0.0	7.46048050409109	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACTACG	35	1.2125747E-7	45.000004	1
TTCGATC	35	1.2125747E-7	45.000004	27
CCTTCGT	20	7.034643E-4	45.0	12
CGAAATA	20	7.034643E-4	45.0	9
GCGCAAC	20	7.034643E-4	45.0	38
TACGATC	20	7.034643E-4	45.0	35
TACGACA	25	3.891931E-5	45.0	21
GTCACGA	25	3.891931E-5	45.0	10
ACTACGC	20	7.034643E-4	45.0	39
GTCGTTA	20	7.034643E-4	45.0	22
TATTACG	20	7.034643E-4	45.0	1
CTACCGT	20	7.034643E-4	45.0	22
TTACGTC	25	3.891931E-5	45.0	35
TCGACCA	25	3.891931E-5	45.0	28
GCGAATC	30	2.1663782E-6	44.999996	9
CGAATCC	30	2.1663782E-6	44.999996	10
CGGTAAT	30	2.1663782E-6	44.999996	6
TAAGCGT	30	2.1663782E-6	44.999996	27
GTACGAC	30	2.1663782E-6	44.999996	9
CGTTTTT	7425	0.0	42.848484	1
>>END_MODULE