FastQCFastQC Report
Thu 26 May 2016
SRR1546318_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546318_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences865962
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33630.38835422339548387No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21140.24412156653525213No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT9390.10843431928883715No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGA207.0323213E-445.00000410
GTTAGCG351.2114651E-745.0000041
TAACGAG207.0323213E-445.0000041
TATGCGT207.0323213E-445.00000420
GTTACGT207.0323213E-445.00000425
ACGTAAG302.1648793E-645.0000041
CGGTAAT207.0323213E-445.0000046
TCAACGT207.0323213E-445.00000438
CGTAAAA253.890006E-545.021
CGTAAGG1350.043.3333362
TTACGGG2350.043.0851063
TTACGAG556.002665E-1140.9090921
CGTTTTT27500.040.827271
CTACGAA1250.039.611
CGAATAT1250.039.614
CTAAGCG403.4568984E-739.3750041
TCTAGCG403.4568984E-739.3750041
TTGCGAG1200.039.3750041
ATAACGC403.4568984E-739.37500411
CGTTTGG1200.039.3750042
Produced by FastQC (version 0.11.5)