FastQCFastQC Report
Thu 26 May 2016
SRR1546317_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546317_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1779756
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73540.4132027086859098No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC59370.33358505323201604No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG850.045.0000041
GTACCAC207.0343306E-445.023
TACCCGA207.0343306E-445.030
TCGGCGA207.0343306E-445.010
CACTCGA253.891671E-545.014
CGTAAGG1800.045.02
CAATACG207.0343306E-445.01
CGGTCTA1950.043.84615731
CGTTTTT60850.042.3746951
TAACGGG5450.042.1100923
ACGTTAG650.041.5384641
ACGTAAG556.184564E-1140.9090921
TAACCGA501.0822987E-940.532
ACGTATG501.0822987E-940.51
TATAACG950.040.2631571
TAGTAAG1900.040.2631571
CGCATCG451.929402E-840.021
TAATGCG451.929402E-840.01
TACGGGA3550.039.9295774
TCTACGA403.459645E-739.37515