##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546317_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1779756
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.10738943990075	31.0	31.0	33.0	30.0	34.0
2	31.444540712322365	31.0	31.0	34.0	30.0	34.0
3	31.55560593699361	31.0	31.0	34.0	30.0	34.0
4	35.391832363537475	37.0	35.0	37.0	33.0	37.0
5	35.29459319142624	37.0	35.0	37.0	33.0	37.0
6	35.330182339601606	37.0	35.0	37.0	33.0	37.0
7	35.74719231175509	37.0	35.0	37.0	35.0	37.0
8	35.64361350657057	37.0	35.0	37.0	35.0	37.0
9	37.352347175680265	39.0	37.0	39.0	35.0	39.0
10	36.88634228512223	39.0	37.0	39.0	32.0	39.0
11	36.58980051198029	39.0	35.0	39.0	32.0	39.0
12	35.77389203913346	37.0	35.0	39.0	31.0	39.0
13	35.403849179325704	37.0	35.0	39.0	30.0	39.0
14	36.29895614904515	38.0	35.0	40.0	31.0	41.0
15	36.61314079008583	38.0	35.0	40.0	31.0	41.0
16	36.727610975886584	38.0	35.0	40.0	32.0	41.0
17	36.67584208172356	38.0	35.0	40.0	31.0	41.0
18	36.58500266328643	38.0	35.0	40.0	31.0	41.0
19	36.47774076895934	38.0	35.0	40.0	31.0	41.0
20	36.322499825818824	38.0	35.0	40.0	31.0	41.0
21	36.15411719359283	37.0	35.0	40.0	30.0	41.0
22	36.02225305041815	37.0	34.0	40.0	30.0	41.0
23	35.960500765273444	37.0	34.0	40.0	30.0	41.0
24	35.93111078147791	37.0	34.0	40.0	30.0	41.0
25	35.85997743510908	37.0	34.0	40.0	30.0	41.0
26	35.73777809991932	37.0	34.0	40.0	30.0	41.0
27	35.61531243608675	37.0	34.0	40.0	30.0	41.0
28	35.59305545254518	37.0	34.0	40.0	29.0	41.0
29	35.54514439057938	36.0	34.0	40.0	29.0	41.0
30	35.42628877216877	36.0	34.0	40.0	29.0	41.0
31	35.2326397551125	36.0	34.0	40.0	28.0	41.0
32	35.03832604019877	36.0	34.0	40.0	27.0	41.0
33	34.85985607015793	36.0	34.0	40.0	26.0	41.0
34	34.67728778551667	36.0	34.0	40.0	25.0	41.0
35	34.50487594928743	36.0	33.0	40.0	24.0	41.0
36	34.28097166128391	36.0	33.0	40.0	23.0	41.0
37	34.07993904782453	36.0	33.0	40.0	23.0	41.0
38	34.022001330519466	35.0	33.0	40.0	23.0	41.0
39	33.95061008362944	35.0	33.0	40.0	23.0	41.0
40	33.7094877050562	35.0	33.0	40.0	23.0	41.0
41	33.616117040762894	35.0	33.0	40.0	22.0	41.0
42	33.47144945711659	35.0	32.0	40.0	22.0	41.0
43	33.430078617518355	35.0	32.0	40.0	22.0	41.0
44	33.24126565664057	35.0	32.0	39.0	21.0	41.0
45	33.19800916530131	35.0	32.0	39.0	22.0	41.0
46	33.11497025434947	35.0	32.0	39.0	21.0	41.0
47	33.04301319956219	35.0	32.0	39.0	21.0	41.0
48	32.97899150220592	35.0	32.0	39.0	20.0	41.0
49	32.993789036249915	35.0	32.0	39.0	20.0	41.0
50	32.84311444939643	35.0	32.0	39.0	20.0	40.0
51	32.615611915341205	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	1.0
11	4.0
12	6.0
13	15.0
14	44.0
15	78.0
16	222.0
17	582.0
18	1390.0
19	2579.0
20	4471.0
21	6976.0
22	10158.0
23	14584.0
24	19582.0
25	26781.0
26	34058.0
27	39629.0
28	43143.0
29	48583.0
30	56036.0
31	67928.0
32	82556.0
33	105862.0
34	178183.0
35	238241.0
36	128818.0
37	159931.0
38	219918.0
39	289336.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.28872047629001	23.689876589824674	28.86429375712176	14.157109176763557
2	29.48921088059262	26.545099440597475	30.405797199166628	13.559892479643276
3	29.24479535396987	25.091417025704647	31.79756101398169	13.86622660634379
4	25.86141021578239	28.10244775126478	30.654876286412296	15.381265746540537
5	22.766491586487135	32.36393078601786	29.536745486459942	15.332832141035063
6	20.950793254805717	40.4119441091925	27.350715491337013	11.286547144664775
7	85.41047199728501	5.300164741683692	7.177837860920261	2.1115254001110264
8	86.74351989823325	4.225972549046049	6.63590964154637	2.394597911174341
9	81.32479957926817	5.760227806508308	9.159570188273	3.7554024259505234
10	51.49368789879062	22.261590914709657	13.79380094799512	12.450920238504604
11	44.31214166436298	20.742000588844764	19.710398504064603	15.235459242727655
12	38.96461087924412	20.416225594969198	25.490741427476575	15.128422098310104
13	22.788741827531414	37.245611196141496	25.45281488024201	14.512832096085083
14	16.802134674640794	39.267967069643255	27.542876663992143	16.387021591723812
15	15.555727863819534	21.9648648466419	46.26173475465176	16.217672534886805
16	17.014860464018664	17.67961450895516	44.13121798718476	21.174307039841416
17	17.7892362773324	18.41331058864249	28.243984006796435	35.553469127228674
18	23.10833619889468	22.049202250196096	32.2151463459036	22.62731520500563
19	29.310197577645475	23.577557822532977	24.938699462173467	22.17354513764808
20	31.149607024783172	22.32339713983265	24.218656939490582	22.308338895893595
21	24.148254030327752	26.437837546270387	26.79625746450637	22.61765095889549
22	23.639813547475047	24.584549792218706	25.09181033804634	26.683826322259907
23	21.60166899282823	28.941776288435044	24.33282989353597	25.12372482520076
24	22.042965440206412	23.624418178671682	35.0547490779635	19.27786730315841
25	19.83771932781797	24.550106868581985	33.07992781032906	22.532245993270987
26	18.829940733448854	31.171520140963143	26.8503098177503	23.148229307837703
27	19.781082350614355	28.969308152353467	28.799003908400927	22.45060558863125
28	17.48571152450111	27.284526642978022	35.79546859232389	19.434293240196972
29	17.886496800685038	24.29568997098479	35.03867945943151	22.779133768898657
30	20.266823092603705	27.133438516290997	30.767644553523066	21.83209383758223
31	28.591615929374587	25.128332198346293	23.8951294447104	22.384922427568725
32	28.952170971751183	25.72599839528565	25.10731808180447	20.2145125511587
33	26.588869485480032	26.179768462643192	25.287567509254078	21.943794542622697
34	20.67406992868685	26.067955382647956	28.54694688485388	24.71102780381131
35	21.25308188313454	24.851665059704814	29.666482371740845	24.228770685419796
36	28.866316506307605	24.39446755622681	25.43129507640373	21.307920861061856
37	22.04667381371379	29.72514209813031	27.509613677380496	20.71857041077541
38	22.472406329856454	30.577730879963323	23.230656337160823	23.719206453019403
39	22.325026576676805	28.690899201913073	25.836013476004577	23.148060745405548
40	24.055488505165876	25.00516924791938	25.001797999276302	25.93754424763844
41	19.155378602460114	23.91468268684022	25.85567909308917	31.074259617610505
42	22.60467165161966	26.229775317515436	23.607730497888475	27.557822532976427
43	23.708025145019878	26.283771483281974	24.90863916177274	25.099564209925408
44	22.056113309914394	28.58313162028952	26.49278889915247	22.867966170643616
45	19.426314618408366	32.10367039077266	23.85489921090307	24.6151157799159
46	23.13429481344634	29.68401286468482	26.19359058207979	20.98810173978905
47	23.46310393110067	25.46034400221154	27.33829805883503	23.738254007852763
48	23.867091893495513	22.992140495663453	28.713318005389503	24.427449605451535
49	21.40343957261557	22.88690135052221	30.843272898082656	24.86638617877956
50	20.30474963983827	29.35256293559342	26.59033035989203	23.752357064676282
51	20.382794045925394	30.477885732650993	24.051049694452498	25.08827052697111
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	770.0
1	1409.5
2	2049.0
3	7732.0
4	13415.0
5	9942.0
6	6469.0
7	6688.0
8	6907.0
9	7150.5
10	7394.0
11	7277.0
12	7160.0
13	7045.5
14	6931.0
15	6699.5
16	6468.0
17	6207.5
18	5947.0
19	5819.0
20	5691.0
21	5616.5
22	5542.0
23	5851.5
24	6161.0
25	7057.5
26	9374.5
27	10795.0
28	12909.0
29	15023.0
30	18944.5
31	22866.0
32	25650.5
33	28435.0
34	32264.0
35	36093.0
36	37863.0
37	39633.0
38	50281.0
39	60929.0
40	82215.5
41	103502.0
42	124734.5
43	145967.0
44	148579.5
45	151192.0
46	146466.0
47	141740.0
48	140776.5
49	139813.0
50	140264.5
51	140716.0
52	133627.5
53	126539.0
54	114381.0
55	102223.0
56	97802.5
57	93382.0
58	87990.0
59	82598.0
60	75923.0
61	69248.0
62	63128.5
63	57009.0
64	50419.0
65	43829.0
66	37004.0
67	30179.0
68	23942.5
69	17706.0
70	15227.0
71	12748.0
72	10588.0
73	8428.0
74	6817.5
75	3822.0
76	2437.0
77	1848.5
78	1260.0
79	1045.5
80	831.0
81	523.5
82	216.0
83	239.5
84	263.0
85	154.5
86	46.0
87	37.0
88	28.0
89	21.0
90	14.0
91	8.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1779756.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.61984466635441
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.75782798916774	16.236957300723486
2	6.311315000400322	2.476540398965737
3	2.002015089950021	1.1783767525355089
4	0.9660422312779291	0.7581439407524556
5	0.6075887810440651	0.5960398752552089
6	0.42037875579725453	0.49486595338664807
7	0.30134905276329615	0.41386951238982367
8	0.23805465919997962	0.373647634848443
9	0.23004516333156583	0.40621053337303065
>10	3.0899932663367378	15.471318951158702
>50	1.807378289820369	26.116134357298336
>100	1.2611078458423115	33.75944828195935
>500	0.005177906301332595	0.6502306296379821
>1k	0.0011506458447405766	0.3180886927037294
>5k	5.753229223702883E-4	0.7501271850115805
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7354	0.4132027086859098	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5937	0.33358505323201604	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.045174731817170445	0.0
2	0.0	0.0	0.0	0.18744142455482662	0.0
3	0.0	0.0	0.0	0.263912581275186	0.0
4	0.0	0.0	0.0	0.4019652132090017	0.0
5	0.0	0.0	0.0	0.6849815367949315	0.0
6	0.0	0.0	0.0	0.9912032885406764	0.0
7	0.0	0.0	0.0	1.1860052726328778	0.0
8	0.0	0.0	0.0	1.7392833624384467	0.0
9	0.0	0.0	0.0	1.9928574478748773	0.0
10	0.0	0.0	0.0	2.396676847837569	0.0
11	0.0	0.0	0.0	2.6928972286088655	0.0
12	0.0	0.0	0.0	2.9696767422051114	0.0
13	0.0	0.0	0.0	3.076039636894046	0.0
14	0.0	0.0	0.0	3.1159889333144544	0.0
15	0.0	0.0	0.0	3.180941657170983	0.0
16	0.0	0.0	0.0	3.326411036119558	0.0
17	0.0	0.0	0.0	3.4895232829668785	0.0
18	0.0	0.0	0.0	3.745063930111768	0.0
19	0.0	0.0	0.0	3.843504390489483	0.0
20	0.0	0.0	0.0	3.9552612830073337	0.0
21	0.0	0.0	0.0	4.091234978277921	0.0
22	0.0	0.0	0.0	4.2241183622923595	0.0
23	0.0	0.0	0.0	4.383746985541839	0.0
24	0.0	0.0	0.0	4.503875812189985	0.0
25	0.0	0.0	0.0	4.606698895803694	0.0
26	0.0	0.0	0.0	4.702273794834798	0.0
27	0.0	0.0	0.0	4.793185133242984	0.0
28	0.0	0.0	0.0	4.894996842263771	0.0
29	0.0	0.0	0.0	4.997988488309633	0.0
30	0.0	0.0	0.0	5.140592305911597	0.0
31	0.0	0.0	0.0	5.265103755795738	0.0
32	0.0	0.0	0.0	5.3814680214591215	0.0
33	0.0	0.0	0.0	5.499405536489272	0.0
34	0.0	0.0	0.0	5.620995237549417	0.0
35	0.0	0.0	0.0	5.776690737381978	0.0
36	0.0	0.0	0.0	5.905809560411652	0.0
37	0.0	0.0	0.0	6.029197260748102	0.0
38	0.0	0.0	0.0	6.149157524964096	0.0
39	0.0	0.0	0.0	6.272545225300547	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	85	0.0	45.000004	1
GTACCAC	20	7.0343306E-4	45.0	23
TACCCGA	20	7.0343306E-4	45.0	30
TCGGCGA	20	7.0343306E-4	45.0	10
CACTCGA	25	3.891671E-5	45.0	14
CGTAAGG	180	0.0	45.0	2
CAATACG	20	7.0343306E-4	45.0	1
CGGTCTA	195	0.0	43.846157	31
CGTTTTT	6085	0.0	42.374695	1
TAACGGG	545	0.0	42.110092	3
ACGTTAG	65	0.0	41.538464	1
ACGTAAG	55	6.184564E-11	40.909092	1
TAACCGA	50	1.0822987E-9	40.5	32
ACGTATG	50	1.0822987E-9	40.5	1
TATAACG	95	0.0	40.263157	1
TAGTAAG	190	0.0	40.263157	1
CGCATCG	45	1.929402E-8	40.0	21
TAATGCG	45	1.929402E-8	40.0	1
TACGGGA	355	0.0	39.929577	4
TCTACGA	40	3.459645E-7	39.375	15
>>END_MODULE