FastQCFastQC Report
Thu 26 May 2016
SRR1546316_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546316_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1004394
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC58820.5856267560339866No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36640.364797081623347No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCG207.03286E-445.0000041
GTGTTCG207.03286E-445.0000041
TTGATAC207.03286E-445.00000442
GTGCACG406.8139343E-945.0000041
CTATGCG406.8139343E-945.0000041
AAATCCG207.03286E-445.00000422
TACCATA207.03286E-445.00000429
CGACGGA207.03286E-445.00000428
ACGCATC207.03286E-445.00000440
TTTCGTC406.8139343E-945.00000412
GACAACG207.03286E-445.00000438
CCGTGTA406.8139343E-945.00000437
GCACGAG406.8139343E-945.0000041
ATTCGAA207.03286E-445.00000414
CGCGACA207.03286E-445.0000042
TGACGAT207.03286E-445.00000424
CCTAACG207.03286E-445.0000041
TAGCCGT406.8139343E-945.00000444
TACGCCC207.03286E-445.00000434
CCTCCGT207.03286E-445.00000416