##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546316_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1004394
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.214826054317328	31.0	31.0	33.0	30.0	34.0
2	31.556747650822288	31.0	31.0	34.0	30.0	34.0
3	31.634114700008165	31.0	31.0	34.0	30.0	34.0
4	35.48945234638996	37.0	35.0	37.0	33.0	37.0
5	35.40469974930157	37.0	35.0	37.0	33.0	37.0
6	35.444481946327834	37.0	35.0	37.0	33.0	37.0
7	35.81867673442892	37.0	35.0	37.0	35.0	37.0
8	35.73200556753625	37.0	35.0	37.0	35.0	37.0
9	37.53341019560053	39.0	37.0	39.0	35.0	39.0
10	37.09244081505863	39.0	37.0	39.0	33.0	39.0
11	36.708022947170136	39.0	35.0	39.0	32.0	39.0
12	35.389588149670345	35.0	35.0	39.0	31.0	39.0
13	34.81549670746739	35.0	34.0	39.0	29.0	39.0
14	35.61606799722021	36.0	34.0	40.0	29.0	41.0
15	36.10470990467884	36.0	35.0	40.0	31.0	41.0
16	36.330310615157	36.0	35.0	40.0	32.0	41.0
17	36.30004062150909	36.0	35.0	40.0	32.0	41.0
18	36.25301126848627	36.0	35.0	40.0	31.0	41.0
19	36.10534610919619	36.0	35.0	40.0	31.0	41.0
20	35.92620724536387	36.0	34.0	40.0	31.0	41.0
21	35.7206355274922	35.0	34.0	40.0	31.0	41.0
22	35.58883466050175	35.0	34.0	40.0	30.0	41.0
23	35.591921098692346	35.0	34.0	40.0	31.0	41.0
24	35.55509192607682	35.0	34.0	40.0	31.0	41.0
25	35.48023086557666	35.0	34.0	40.0	30.0	41.0
26	35.36976525148498	35.0	34.0	40.0	30.0	41.0
27	35.25107079492709	35.0	34.0	40.0	30.0	41.0
28	35.25811982150431	35.0	34.0	40.0	30.0	41.0
29	35.29219310350321	35.0	34.0	40.0	30.0	41.0
30	35.22223350597475	36.0	34.0	40.0	30.0	41.0
31	34.94798754273721	35.0	34.0	40.0	29.0	41.0
32	34.716483770313246	35.0	34.0	40.0	29.0	41.0
33	34.519995141348915	35.0	34.0	40.0	27.0	41.0
34	34.332522894402	35.0	34.0	40.0	26.0	41.0
35	34.16656013476783	35.0	33.0	40.0	25.0	41.0
36	33.89889425862759	35.0	33.0	39.0	23.0	41.0
37	33.635595194714426	35.0	33.0	39.0	23.0	41.0
38	33.65773690404363	35.0	33.0	39.0	23.0	41.0
39	33.66645559411944	35.0	33.0	39.0	23.0	41.0
40	33.413680288810966	35.0	33.0	39.0	23.0	41.0
41	33.394624022047125	35.0	33.0	39.0	23.0	41.0
42	33.30450301375755	35.0	33.0	39.0	23.0	41.0
43	33.18000505777613	35.0	32.0	39.0	23.0	41.0
44	33.031755466480284	35.0	32.0	38.0	23.0	41.0
45	32.99754180132498	35.0	32.0	38.0	23.0	40.0
46	32.95871938701346	35.0	32.0	38.0	23.0	40.0
47	32.916131518109424	35.0	32.0	38.0	23.0	40.0
48	32.91816657606477	35.0	32.0	38.0	23.0	40.0
49	32.984953116008256	35.0	32.0	38.0	23.0	40.0
50	32.81504369799103	35.0	32.0	38.0	22.0	40.0
51	32.58758116834629	35.0	32.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	7.0
14	23.0
15	38.0
16	112.0
17	240.0
18	551.0
19	1177.0
20	2076.0
21	3372.0
22	4915.0
23	7310.0
24	10493.0
25	15293.0
26	19663.0
27	22608.0
28	24415.0
29	27002.0
30	31407.0
31	38687.0
32	49238.0
33	65192.0
34	125725.0
35	181362.0
36	61069.0
37	71968.0
38	101561.0
39	138870.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.473604979719116	23.811074140227838	29.70398070876568	15.011340171287364
2	29.698006957429058	24.98939659137749	32.149136693369336	13.16345975782412
3	26.77684255381852	24.990093529033427	33.994727168820205	14.238336748327848
4	24.78031529459555	27.747577146020387	32.20080964243115	15.271297916952909
5	22.123887637719857	32.69951831651722	31.121751025991788	14.054843019771127
6	20.433116884409902	39.21100683596278	29.5681774283797	10.787698851247619
7	85.82697626628594	5.262277552434602	7.237797119457105	1.6729490618223524
8	88.3647253966073	3.4351061436049997	6.4326350018020815	1.7675334579856112
9	84.89477237020532	4.542241391326511	8.09084881032742	2.4721374281407495
10	61.55223945981357	17.369578073943092	11.327029034422747	9.75115343182058
11	57.16521604071709	15.576755735299097	16.68140191996368	10.576626304020136
12	48.557637739771444	20.002907225650493	20.73966989050114	10.699785144076927
13	20.445761324739095	47.61089771543837	21.95612478768292	9.987216172139618
14	12.783130922725544	48.8049510451078	27.06358261797661	11.348335414190048
15	11.101519921465082	19.619491952361322	57.34582245612777	11.933165670045819
16	12.512918237265454	14.17750404721653	54.26973876785405	19.039838947663963
17	13.581821476432554	14.880515017015236	28.797663068477114	42.7400004380751
18	21.317232082230678	20.332260049343187	36.217659603701335	22.1328482647248
19	31.17601260063282	21.315539519351965	25.93812786615611	21.570320013859103
20	33.61310402093202	20.762569270624876	22.764472906050813	22.859853802392287
21	22.76188428047161	27.740209519371877	27.136561946805738	22.361344253350776
22	22.922578191426872	24.850805560367746	24.216691855984802	28.00992439222058
23	18.47731069679827	32.608617733678216	23.587257590148887	25.32681397937463
24	20.446458262395037	22.813855917100263	40.55380657391422	16.18587924659048
25	15.215343779433171	24.053011069361226	39.49197227382879	21.239672877376805
26	15.10771669285161	36.29900218440174	27.834893478057417	20.758387644689236
27	17.51464066890085	33.411091663231765	29.15031352238265	19.92395414548474
28	12.835102559354198	26.879192826719393	43.60330706873995	16.68239754518645
29	13.58271753913305	23.005115522394597	42.242685639300916	21.16948129917144
30	17.186283470430926	29.113077139051008	32.78424602297505	20.916393367543016
31	30.56838252717559	24.37519539144997	24.307592438823807	20.748829642550632
32	32.06958623806992	26.084982586514855	24.761298852840618	17.084132322574607
33	29.27795267594191	25.18682907305301	25.26727559105291	20.26794265995217
34	17.94494989018254	25.96680187257192	30.445422812163354	25.64282542508219
35	19.221739675864253	24.739594222984206	32.597665856227735	23.441000244923803
36	32.33053960895823	21.36362821761181	26.10111171512375	20.204720458306202
37	20.57051316515232	31.455882850753785	28.101521912715526	19.872082071378365
38	20.492057897597952	33.5026891837267	22.089239879967423	23.916013038707916
39	20.14279256944984	31.89853782479784	25.719189879668736	22.239479726083587
40	24.367130827145523	25.166418755986196	23.030802653142093	27.43564776372619
41	16.268715265125042	22.611445309310888	25.560785906725847	35.559053518838226
42	22.802406227038393	25.080994111872435	22.562460548350547	29.554139112738625
43	23.487197255260387	26.321543139445275	24.135747525373507	26.055512079920828
44	20.447553450140084	31.425416718936987	26.85151444552636	21.275515385396567
45	16.384506478533325	37.97145343361271	21.165996610891742	24.478043476962227
46	21.79752168969548	33.440960419914894	25.92777336383929	18.83374452655034
47	22.281395547962255	25.318649852547903	27.263305037664505	25.13664956182534
48	24.267866992435238	21.131348853139308	30.416748805747545	24.18403534867791
49	20.44566176221682	20.54452734683799	33.941461219401944	25.06834967154324
50	18.385713176303323	33.6541237801102	24.725555907343132	23.234607136243348
51	17.602454813549265	35.93729154096898	21.905746151410703	24.55450749407105
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	406.0
1	549.0
2	692.0
3	3616.5
4	6541.0
5	5177.5
6	3814.0
7	4260.5
8	4707.0
9	5130.5
10	5554.0
11	5548.5
12	5543.0
13	5401.0
14	5259.0
15	4903.0
16	4547.0
17	4180.0
18	3813.0
19	3578.5
20	3344.0
21	3275.5
22	3207.0
23	3123.5
24	3040.0
25	3206.0
26	3889.0
27	4406.0
28	5016.0
29	5626.0
30	6841.0
31	8056.0
32	10853.5
33	13651.0
34	15050.5
35	16450.0
36	16607.0
37	16764.0
38	25069.0
39	33374.0
40	57834.0
41	82294.0
42	95959.5
43	109625.0
44	114126.0
45	118627.0
46	104428.0
47	90229.0
48	85731.5
49	81234.0
50	80746.0
51	80258.0
52	76303.5
53	72349.0
54	63073.0
55	53797.0
56	48319.0
57	42841.0
58	38426.5
59	34012.0
60	29316.0
61	24620.0
62	23249.5
63	21879.0
64	18173.5
65	14468.0
66	12433.5
67	10399.0
68	8546.5
69	6694.0
70	5414.5
71	4135.0
72	3373.0
73	2611.0
74	1861.5
75	843.5
76	575.0
77	399.0
78	223.0
79	136.5
80	50.0
81	95.5
82	141.0
83	85.5
84	30.0
85	20.5
86	11.0
87	9.5
88	8.0
89	5.0
90	2.0
91	2.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1004394.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.203416833584875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.0469697252345	15.117386066745475
2	6.34677871720513	2.3106611707962017
3	1.9097058622793595	1.0428951552183545
4	0.90474721025805	0.6587796238940138
5	0.5398726860276416	0.49137637704141274
6	0.3689024437675616	0.40291709728974173
7	0.3094741415790986	0.3943440758866135
8	0.21815528272472376	0.3176937236709364
9	0.19122367593284098	0.3132831853310271
>10	2.6713142651467776	12.534397176020518
>50	1.906987581527797	26.036464406053
>100	1.582580498693183	39.12591952444707
>500	0.002191939748882525	0.3016499944979705
>1k	5.479849372206312E-4	0.3654912631747896
>5k	5.479849372206312E-4	0.586741159932891
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5882	0.5856267560339866	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3664	0.364797081623347	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.060434451022208416	0.0
2	0.0	0.0	0.0	0.19464473105175858	0.0
3	0.0	0.0	0.0	0.26025643323237696	0.0
4	0.0	0.0	0.0	0.37664502177432363	0.0
5	0.0	0.0	0.0	0.588514069180023	0.0
6	0.0	0.0	0.0	0.8364247496500378	0.0
7	0.0	0.0	0.0	1.0035902245533128	0.0
8	0.0	0.0	0.0	1.4768108929364372	0.0
9	0.0	0.0	0.0	1.7000300678817277	0.0
10	0.0	0.0	0.0	2.0611433361808214	0.0
11	0.0	0.0	0.0	2.3537575891532607	0.0
12	0.0	0.0	0.0	2.621282086511867	0.0
13	0.0	0.0	0.0	2.7272166102147164	0.0
14	0.0	0.0	0.0	2.768535056959719	0.0
15	0.0	0.0	0.0	2.8278743202368792	0.0
16	0.0	0.0	0.0	2.956708224063465	0.0
17	0.0	0.0	0.0	3.1173025724964507	0.0
18	0.0	0.0	0.0	3.384926632377334	0.0
19	0.0	0.0	0.0	3.469554776312881	0.0
20	0.0	0.0	0.0	3.567723423278116	0.0
21	0.0	0.0	0.0	3.6912805134240148	0.0
22	0.0	0.0	0.0	3.8078682270105157	0.0
23	0.0	0.0	0.0	3.955121197458368	0.0
24	0.0	0.0	0.0	4.05338940694588	0.0
25	0.0	0.0	0.0	4.138515363492813	0.0
26	0.0	0.0	0.0	4.224139132651131	0.0
27	0.0	0.0	0.0	4.30179790002728	0.0
28	0.0	0.0	0.0	4.38324004324996	0.0
29	0.0	0.0	0.0	4.468863812408278	0.0
30	0.0	0.0	0.0	4.6005850293809	0.0
31	0.0	0.0	0.0	4.718964868368389	0.0
32	0.0	0.0	0.0	4.80867070094007	0.0
33	0.0	0.0	0.0	4.912713536719654	0.0
34	0.0	0.0	0.0	5.024024436625468	0.0
35	0.0	0.0	0.0	5.18023803407826	0.0
36	0.0	0.0	0.0	5.295033622263773	0.0
37	0.0	0.0	0.0	5.403158521456719	0.0
38	0.0	0.0	0.0	5.504712294179376	0.0
39	0.0	0.0	0.0	5.609651192659454	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTTCG	20	7.03286E-4	45.000004	1
GTGTTCG	20	7.03286E-4	45.000004	1
TTGATAC	20	7.03286E-4	45.000004	42
GTGCACG	40	6.8139343E-9	45.000004	1
CTATGCG	40	6.8139343E-9	45.000004	1
AAATCCG	20	7.03286E-4	45.000004	22
TACCATA	20	7.03286E-4	45.000004	29
CGACGGA	20	7.03286E-4	45.000004	28
ACGCATC	20	7.03286E-4	45.000004	40
TTTCGTC	40	6.8139343E-9	45.000004	12
GACAACG	20	7.03286E-4	45.000004	38
CCGTGTA	40	6.8139343E-9	45.000004	37
GCACGAG	40	6.8139343E-9	45.000004	1
ATTCGAA	20	7.03286E-4	45.000004	14
CGCGACA	20	7.03286E-4	45.000004	2
TGACGAT	20	7.03286E-4	45.000004	24
CCTAACG	20	7.03286E-4	45.000004	1
TAGCCGT	40	6.8139343E-9	45.000004	44
TACGCCC	20	7.03286E-4	45.000004	34
CCTCCGT	20	7.03286E-4	45.000004	16
>>END_MODULE