##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546315_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1675932
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.153343333739077	31.0	31.0	33.0	30.0	34.0
2	31.489642777869268	31.0	31.0	34.0	30.0	34.0
3	31.605025144218263	31.0	31.0	34.0	30.0	34.0
4	35.446115355515616	37.0	35.0	37.0	33.0	37.0
5	35.33679707768573	37.0	35.0	37.0	33.0	37.0
6	35.38154411992849	37.0	35.0	37.0	33.0	37.0
7	35.785844533071746	37.0	35.0	37.0	35.0	37.0
8	35.69754321774392	37.0	35.0	37.0	35.0	37.0
9	37.48381855588413	39.0	37.0	39.0	35.0	39.0
10	36.915967354284064	39.0	37.0	39.0	32.0	39.0
11	36.62243336841829	39.0	35.0	39.0	32.0	39.0
12	35.78185093428612	37.0	35.0	39.0	31.0	39.0
13	35.365733812589056	37.0	35.0	39.0	30.0	39.0
14	36.268757324282845	38.0	35.0	40.0	30.0	41.0
15	36.60807359725812	38.0	35.0	40.0	31.0	41.0
16	36.74413341352752	38.0	35.0	40.0	32.0	41.0
17	36.70717427676063	38.0	35.0	40.0	32.0	41.0
18	36.66451204464143	38.0	35.0	40.0	31.0	41.0
19	36.57970072771449	38.0	35.0	40.0	31.0	41.0
20	36.400751939816175	38.0	35.0	40.0	31.0	41.0
21	36.24379032084834	37.0	35.0	40.0	31.0	41.0
22	36.14302847609569	37.0	35.0	40.0	30.0	41.0
23	36.10393560120578	37.0	35.0	40.0	31.0	41.0
24	36.04419093376104	37.0	35.0	40.0	30.0	41.0
25	35.9746600697403	37.0	34.0	40.0	30.0	41.0
26	35.8610051004456	37.0	34.0	40.0	30.0	41.0
27	35.73123312879043	37.0	34.0	40.0	30.0	41.0
28	35.748393729578524	37.0	34.0	40.0	30.0	41.0
29	35.6908293415246	37.0	34.0	40.0	30.0	41.0
30	35.56930233446226	36.0	34.0	40.0	29.0	41.0
31	35.351056606115286	36.0	34.0	40.0	29.0	41.0
32	35.181757374404214	36.0	34.0	40.0	28.0	41.0
33	35.03252518598607	36.0	34.0	40.0	27.0	41.0
34	34.86222651038348	36.0	34.0	40.0	27.0	41.0
35	34.70773098192528	36.0	34.0	40.0	26.0	41.0
36	34.47984166422027	36.0	33.0	40.0	24.0	41.0
37	34.276604898050756	36.0	33.0	40.0	24.0	41.0
38	34.215117319795795	35.0	33.0	40.0	24.0	41.0
39	34.19640773014657	36.0	33.0	40.0	23.0	41.0
40	33.99902382674237	35.0	33.0	40.0	23.0	41.0
41	33.93795094311702	35.0	33.0	40.0	23.0	41.0
42	33.83173959325319	35.0	33.0	40.0	23.0	41.0
43	33.75693763231444	35.0	33.0	40.0	23.0	41.0
44	33.61110653654205	35.0	33.0	40.0	23.0	41.0
45	33.55319129893098	35.0	32.0	39.0	23.0	41.0
46	33.520683416749605	35.0	32.0	39.0	23.0	41.0
47	33.43765260165687	35.0	32.0	39.0	23.0	41.0
48	33.358386259108364	35.0	32.0	39.0	23.0	41.0
49	33.37557848409124	35.0	32.0	39.0	23.0	41.0
50	33.21393767766234	35.0	32.0	39.0	22.0	41.0
51	32.98646902141614	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	3.0
12	1.0
13	6.0
14	24.0
15	55.0
16	193.0
17	417.0
18	1000.0
19	1997.0
20	3553.0
21	5615.0
22	8457.0
23	12183.0
24	16784.0
25	22965.0
26	30227.0
27	34782.0
28	38403.0
29	43071.0
30	50989.0
31	61568.0
32	75718.0
33	98338.0
34	170047.0
35	234112.0
36	119189.0
37	148590.0
38	211771.0
39	285833.0
40	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.611943682679254	23.57345047412425	30.010167476962074	13.804438366234429
2	29.490874331416787	25.34476339135478	32.14098185367903	13.023380423549405
3	28.36809608027056	25.04546723852758	32.189193833640026	14.397242847561834
4	25.827420205593064	27.495924655654285	31.25449003897533	15.422165099777319
5	22.388796204141933	32.369690417033624	30.84719427757212	14.394319101252318
6	20.359895270213826	40.74485122308065	27.889138700138194	11.00611480656733
7	87.76507638734746	4.3787576106906485	6.139449571939673	1.7167164300222204
8	89.71139640510474	3.13550907793395	5.335240331946642	1.8178541850146663
9	85.58479699653685	4.012215292744575	7.753894549420859	2.6490931612977136
10	51.41002140898318	20.156187721220192	14.898695173789866	13.53509569600676
11	46.44860292661039	21.40146497590594	18.53315050968655	13.616781587797119
12	41.10823112154908	21.69395894344162	22.258361317762297	14.939448617247
13	22.983987417150576	38.131618705293526	22.788693097333304	16.095700780222586
14	17.805137678617033	40.65642281429079	25.58116916438137	15.957270342710803
15	15.836680724516269	22.443452359642276	45.81898310909989	15.900883806741561
16	17.110061744748595	16.73588188542256	44.480444313969784	21.673612055859067
17	17.391039731922298	18.541862080323067	27.90745686579169	36.159641321962944
18	22.342553277817952	22.71864252248898	33.94821508271219	20.99058911698088
19	30.378380507085012	24.43589596713948	23.745832169801638	21.43989135597387
20	31.850456939780376	23.275586360305788	22.710109956728555	22.163846743185285
21	23.790702725408906	27.596585064310485	27.03868653382118	21.574025676459428
22	23.474878455689133	24.02639247893113	24.235231501039422	28.263497564340316
23	21.290899630772607	30.549568836921786	22.924975476331973	25.234556055973634
24	22.055906802901312	24.117446292570342	35.71612690729695	18.110519997231393
25	19.90719193857507	24.285054524885258	33.25701758782576	22.55073594871391
26	18.888773530190964	32.22105670158455	25.832551678707727	23.057618089516758
27	19.426325173097716	31.7791533308034	27.869209490599857	20.92531200549903
28	17.67446411906927	26.910817383998875	35.91625435876873	19.498464138163126
29	17.926264311439844	24.121802077888603	35.35376136979305	22.598172240878508
30	21.23152968020182	28.520608234701644	29.931703672941385	20.316158412155147
31	29.31210812849209	25.48414852153906	23.811765632495828	21.391977717473022
32	29.93516443387918	26.51336689078077	24.654102911096633	18.897365764243418
33	28.328715007530135	26.493616686118532	24.6091130189053	20.56855528744603
34	20.63114732578649	26.905805247468273	27.803634037657854	24.659413389087387
35	21.70672795793624	24.479453820322068	30.239592059821042	23.57422616192065
36	30.734480873925673	24.90178599131707	24.606129604303757	19.7576035304535
37	21.410892566046833	31.100307172367376	27.706613394815545	19.782186866770253
38	22.658138874369605	31.23384480993262	23.338238066938278	22.769778248759494
39	20.956816863691365	29.512295248255892	26.290326815169113	23.240561072883626
40	24.138807541117423	24.38834033839082	25.501094316475847	25.971757804015915
41	18.982810758431725	23.382511939625235	26.651856996584588	30.982820305358448
42	23.327139764620522	25.602649749512512	23.650601575720255	27.419608910146714
43	23.00695970958249	26.42010535033641	25.360456152158918	25.212478787922183
44	22.630691460035372	28.761131119878375	26.534370129575663	22.073807290510594
45	18.797182701923468	33.81968958167754	23.271230574987527	24.111897141411465
46	23.35470651553882	29.967683653036044	26.01943276934864	20.658177062076504
47	23.457813324168285	27.231534453665184	25.838757181078947	23.47189504108759
48	23.92764145561992	24.04811173723039	28.918476405963965	23.10577040118573
49	21.452421697300366	23.365208134936264	31.067787953210512	24.114582214552858
50	20.847086874646465	31.02870522193025	25.534806901473328	22.58940100194996
51	20.12414584839958	31.77169479429953	24.419248513662843	23.684910843638047
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	514.0
1	863.0
2	1212.0
3	5454.0
4	9696.0
5	7310.0
6	4924.0
7	5352.5
8	5781.0
9	6132.5
10	6484.0
11	6588.0
12	6692.0
13	6402.0
14	6112.0
15	5902.0
16	5692.0
17	5294.5
18	4897.0
19	4540.0
20	4183.0
21	4086.5
22	3990.0
23	4404.0
24	4818.0
25	5067.0
26	5627.5
27	5939.0
28	8230.0
29	10521.0
30	14273.5
31	18026.0
32	19335.5
33	20645.0
34	27143.5
35	33642.0
36	35826.5
37	38011.0
38	50679.0
39	63347.0
40	83968.5
41	104590.0
42	126811.5
43	149033.0
44	151317.5
45	153602.0
46	153741.5
47	153881.0
48	150131.0
49	146381.0
50	141227.5
51	136074.0
52	126789.0
53	117504.0
54	106167.5
55	94831.0
56	90549.0
57	86267.0
58	79627.5
59	72988.0
60	68602.5
61	64217.0
62	55482.0
63	46747.0
64	40471.0
65	34195.0
66	27954.0
67	21713.0
68	16872.0
69	12031.0
70	10071.5
71	8112.0
72	6882.0
73	5652.0
74	4902.5
75	2955.0
76	1757.0
77	1190.0
78	623.0
79	575.5
80	528.0
81	435.0
82	342.0
83	247.0
84	152.0
85	101.5
86	51.0
87	47.0
88	43.0
89	26.0
90	9.0
91	7.5
92	6.0
93	5.5
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1675932.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.196778083451253
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.39542434849581	14.81134473877923
2	6.526820227499392	2.3753419854077453
3	2.1465005267605464	1.1717818122441865
4	1.0375865532050823	0.7552292900418389
5	0.6602478142208873	0.6007191477730618
6	0.5075836135002442	0.5541831824196141
7	0.3678898653157548	0.4686087166811092
8	0.30004170920958256	0.43678339186129495
9	0.25235611685769543	0.41328614308148776
>10	3.5584768995522604	16.29002624773243
>50	1.8017312209899554	24.253260986060628
>100	1.4377583385621568	36.09702676544817
>500	0.0046155965924946476	0.49361527453414356
>1k	0.0023077982962473238	0.5624262516720461
>5k	6.593709417849496E-4	0.7163660662630296
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6487	0.38706821040471806	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5454	0.3254308647367554	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1876	0.11193771585004643	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03920206786432862	0.0
2	0.0	0.0	0.0	0.15024475933391093	0.0
3	0.0	0.0	0.0	0.20734731480752203	0.0
4	0.0	0.0	0.0	0.32316346963957965	0.0
5	0.0	0.0	0.0	0.5470985696317034	0.0
6	0.0	0.0	0.0	0.7996744497986792	0.0
7	0.0	0.0	0.0	0.9552296871233439	0.0
8	0.0	0.0	0.0	1.4397958867066205	0.0
9	0.0	0.0	0.0	1.6566901282390933	0.0
10	0.0	0.0	0.0	2.010761773150701	0.0
11	0.0	0.0	0.0	2.28827899938661	0.0
12	0.0	0.0	0.0	2.533336674757687	0.0
13	0.0	0.0	0.0	2.622600439635976	0.0
14	0.0	0.0	0.0	2.653866624660189	0.0
15	0.0	0.0	0.0	2.7067327313996032	0.0
16	0.0	0.0	0.0	2.8402703689648505	0.0
17	0.0	0.0	0.0	2.9912908160951637	0.0
18	0.0	0.0	0.0	3.230023652510961	0.0
19	0.0	0.0	0.0	3.314752627194898	0.0
20	0.0	0.0	0.0	3.4184561187446745	0.0
21	0.0	0.0	0.0	3.5494876880446222	0.0
22	0.0	0.0	0.0	3.6852330524150143	0.0
23	0.0	0.0	0.0	3.853139626190084	0.0
24	0.0	0.0	0.0	3.970805498075101	0.0
25	0.0	0.0	0.0	4.057384189811997	0.0
26	0.0	0.0	0.0	4.155717535078989	0.0
27	0.0	0.0	0.0	4.242654236568071	0.0
28	0.0	0.0	0.0	4.339436206242258	0.0
29	0.0	0.0	0.0	4.44158832219923	0.0
30	0.0	0.0	0.0	4.580734779215386	0.0
31	0.0	0.0	0.0	4.712064689975488	0.0
32	0.0	0.0	0.0	4.82495709849803	0.0
33	0.0	0.0	0.0	4.943338989887418	0.0
34	0.0	0.0	0.0	5.066792686099436	0.0
35	0.0	0.0	0.0	5.230761152600463	0.0
36	0.0	0.0	0.0	5.359286653635111	0.0
37	0.0	0.0	0.0	5.494554671669256	0.0
38	0.0	0.0	0.0	5.629405011659184	0.0
39	0.0	0.0	0.0	5.807574531663576	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	85	0.0	45.000004	1
AATCGCC	30	2.1660999E-6	45.000004	40
TTTCGTA	20	7.0342125E-4	45.0	1
TTAGACG	90	0.0	45.0	1
TATATCG	25	3.8915732E-5	45.0	1
GTATCCG	20	7.0342125E-4	45.0	22
CGTAAAC	20	7.0342125E-4	45.0	10
ATCGGAT	35	1.2123746E-7	45.0	14
CGCGTAG	20	7.0342125E-4	45.0	38
CAATCGT	25	3.8915732E-5	45.0	39
TGCCGAT	25	3.8915732E-5	45.0	34
CGTTTTT	5325	0.0	42.507042	1
GTTTACG	85	0.0	42.352943	1
CGGTCTA	250	0.0	42.3	31
TAGACGG	285	0.0	41.842106	2
TATGGGT	655	0.0	40.190838	4
ACGGGTA	185	0.0	40.135136	5
TAGGGTA	875	0.0	39.85714	5
GTCTAAA	195	0.0	39.23077	33
CGACGGT	280	0.0	38.571426	28
>>END_MODULE