FastQCFastQC Report
Thu 26 May 2016
SRR1546314_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546314_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3465243
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175710.5070640067666251No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC132840.38334973910920533No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC40260.1161823283388784No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT37880.1093141231365304No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATCG406.8212103E-945.0000049
ACTACGC406.8212103E-945.00000417
GAACGTA406.8212103E-945.0000049
CGTAAGT207.0352555E-445.00000435
ACCGTAC351.212884E-745.00000431
TACGCTA253.8924387E-545.037
TATTACG950.045.01
TCGACTA302.1667747E-644.9999962
CGGTCTA4400.043.46590831
CGTTTTT134950.042.749171
ACGTGCG700.041.7857171
CGACGGT4700.040.6914928
ACAATCG501.0822987E-940.51
CTTACGT501.0822987E-940.524
ATGCTCG451.9303116E-840.0000041
GTCGCAA800.039.37500444
ATTGCGG7850.038.9808922
CACGACG5050.038.7623826
GCGCGAC12150.038.7037059
CTACAGT17800.038.67977528