##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546314_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3465243
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.10565983395681	31.0	31.0	33.0	30.0	34.0
2	31.440722627532903	31.0	31.0	34.0	30.0	34.0
3	31.52319707449088	31.0	31.0	34.0	30.0	34.0
4	35.374954079699464	37.0	35.0	37.0	33.0	37.0
5	35.260303534268736	37.0	35.0	37.0	33.0	37.0
6	35.28873386368575	37.0	35.0	37.0	32.0	37.0
7	35.74806211281575	37.0	35.0	37.0	35.0	37.0
8	35.65427215349688	37.0	35.0	37.0	35.0	37.0
9	37.35939557485579	39.0	37.0	39.0	35.0	39.0
10	36.883670495835354	39.0	37.0	39.0	32.0	39.0
11	36.56506599970045	39.0	35.0	39.0	32.0	39.0
12	35.713217514615856	37.0	35.0	39.0	31.0	39.0
13	35.29963468651405	37.0	35.0	39.0	30.0	39.0
14	36.18735078607763	38.0	35.0	40.0	30.0	41.0
15	36.52749951446407	38.0	35.0	40.0	31.0	41.0
16	36.663867728756685	38.0	35.0	40.0	32.0	41.0
17	36.60542305402536	38.0	35.0	40.0	31.0	41.0
18	36.52375663120883	38.0	35.0	40.0	31.0	41.0
19	36.397736608947774	37.0	35.0	40.0	31.0	41.0
20	36.23019453469786	37.0	35.0	40.0	31.0	41.0
21	36.040963072431	37.0	34.0	40.0	30.0	41.0
22	35.92522025150906	37.0	34.0	40.0	30.0	41.0
23	35.88690951832238	37.0	34.0	40.0	30.0	41.0
24	35.86232913535934	37.0	34.0	40.0	30.0	41.0
25	35.76492413374762	36.0	34.0	40.0	30.0	41.0
26	35.6299742904033	36.0	34.0	40.0	30.0	41.0
27	35.50324609269826	36.0	34.0	40.0	29.0	41.0
28	35.43571864945691	36.0	34.0	40.0	29.0	41.0
29	35.42115863158803	36.0	34.0	40.0	29.0	41.0
30	35.29361202085972	36.0	34.0	40.0	29.0	41.0
31	35.0861327762584	36.0	34.0	40.0	28.0	41.0
32	34.87049017918801	36.0	34.0	40.0	27.0	41.0
33	34.698056384501754	36.0	33.0	40.0	26.0	41.0
34	34.4944778764433	36.0	34.0	40.0	25.0	41.0
35	34.30282089885183	36.0	33.0	40.0	24.0	41.0
36	34.04955294621474	35.0	33.0	40.0	23.0	41.0
37	33.836912447409894	35.0	33.0	40.0	23.0	41.0
38	33.75088211706942	35.0	33.0	40.0	23.0	41.0
39	33.69958989889021	35.0	33.0	40.0	23.0	41.0
40	33.48576795335854	35.0	32.0	40.0	22.0	41.0
41	33.40070609766761	35.0	32.0	40.0	21.0	41.0
42	33.2591979840952	35.0	32.0	39.0	21.0	41.0
43	33.18763186304683	35.0	32.0	39.0	21.0	41.0
44	33.011166605054825	35.0	32.0	39.0	20.0	41.0
45	32.988332419977475	35.0	32.0	39.0	20.0	41.0
46	32.905458001069476	35.0	32.0	39.0	20.0	41.0
47	32.841478360969205	35.0	31.0	39.0	20.0	41.0
48	32.760432385261296	35.0	31.0	39.0	20.0	41.0
49	32.77424267215892	35.0	31.0	39.0	20.0	41.0
50	32.60918498356392	35.0	31.0	39.0	19.0	40.0
51	32.36883704836861	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	7.0
11	3.0
12	8.0
13	14.0
14	71.0
15	182.0
16	437.0
17	1170.0
18	2618.0
19	5018.0
20	8797.0
21	13600.0
22	20550.0
23	28904.0
24	40328.0
25	55726.0
26	71250.0
27	82786.0
28	89392.0
29	98378.0
30	113119.0
31	135040.0
32	163463.0
33	208367.0
34	351983.0
35	479584.0
36	244948.0
37	295518.0
38	403761.0
39	550097.0
40	122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.55211827857383	22.8949023199816	29.449190143375226	15.103789258069348
2	29.929964507539587	26.88137599585368	29.65056130262726	13.53809819397947
3	27.80056117276624	25.717503794106218	31.96638735003577	14.515547683091778
4	26.16015673359704	27.404773633479675	30.921236981071747	15.513832651851544
5	21.928794026854682	32.184553868228	31.3221901032626	14.564462001654718
6	21.11975985522516	39.99044222872682	27.923496274287256	10.966301641760765
7	83.88309275857422	5.434250931319968	8.302909781507386	2.379746528598427
8	84.61008362184124	4.364369252026481	8.004864305331544	3.0206828208007344
9	79.5417810525842	6.168658301885322	10.39987671860242	3.8896839269280683
10	50.77179291611007	21.79659550571201	15.423535954044205	12.008075624133719
11	44.650721464555296	19.926077334259098	20.86482823859683	14.55837296258877
12	39.779461353792506	20.785959310790037	25.17047145034273	14.264107885074726
13	22.26602867389098	37.4047361180731	25.308037560425056	15.02119764761086
14	15.961160588160773	40.25711905341126	28.397344717239164	15.3843756411888
15	15.540180010463914	22.16730543860849	47.08278755631279	15.209726994614808
16	15.835916846235603	18.207554275414452	45.20606491377372	20.75046396457622
17	16.647894534380416	19.107808601012973	29.105722167247723	35.13857469735889
18	22.328390822808096	22.34071319096525	34.215262825723904	21.115633160502743
19	28.870558284079934	24.63905128731232	25.264202250751243	21.226188177856503
20	30.56394602052439	22.377766869451868	25.018158899678898	22.040128210344847
21	22.68946218201725	27.465808314164402	27.738227881854172	22.106501621964174
22	22.4916982733967	24.80391707017372	26.04478242939961	26.659602227029964
23	20.417558018297708	29.469073308855975	24.619081547816414	25.494287125029903
24	21.798067263969656	23.651847792492475	35.391197673583065	19.158887269954803
25	19.41606981097718	24.248948775020974	33.567689192359666	22.767292221642176
26	17.9154535482793	31.9247452487459	27.24469827945688	22.915102923517917
27	20.234165396192992	29.289316795387798	29.2103901515709	21.266127656848308
28	16.489031216569806	27.39129694512044	37.07820779091106	19.041464047398694
29	17.255557546757906	23.850390867249423	36.48021221022595	22.41383937576672
30	19.561369866413408	27.10768047147054	31.528322833348195	21.802626828767853
31	28.40251030014345	25.58671931521108	24.158219207137854	21.85255117750761
32	27.858969774991248	26.637699001195585	25.582563762483613	19.920767461329554
33	25.893422192902488	26.545526533059878	25.4535684799017	22.107482794135937
34	19.401583092441136	26.437366730125422	29.85724233480884	24.3038078426246
35	20.763882936925345	24.706146149057943	29.956831310242887	24.573139603773818
36	28.04337242727278	23.955664869678692	27.130449437456477	20.870513265592052
37	20.98565670574906	29.419062386101064	29.26972220995757	20.325558698192307
38	21.076992291738268	31.076262184210456	24.52119519468043	23.325550329370838
39	21.233546969144733	28.387677285546786	27.769134805264738	22.609640940043743
40	23.15502837751927	24.517270505993373	26.480509447677985	25.847191668809373
41	18.4359653854001	23.188763385424917	27.21422422612209	31.16104700305289
42	22.550886041758112	25.772391719714893	24.484747534299903	27.19197470422709
43	22.70374689451793	26.019675965004474	26.511618377123913	24.764958763353683
44	20.788787395285123	29.20730234503035	27.606433372782224	22.397476886902304
45	19.01286576439228	32.76788958234675	23.71221873906101	24.507025914199957
46	22.81502336199799	30.368173314252424	26.272962675344846	20.543840648404743
47	22.208803249873096	25.919798409519913	27.8076025259989	24.063795814608095
48	23.36266172386756	22.598011164007833	30.05064868466656	23.988678427458048
49	20.936944393221484	22.178560060578725	31.95579646218173	24.92869908401806
50	19.24647131528727	28.98154617150947	28.196839298138688	23.57514321506457
51	18.94981102335392	30.735737724598245	25.03276105023515	25.281690201812683
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2155.0
1	3208.0
2	4261.0
3	17650.0
4	31039.0
5	23050.0
6	15061.0
7	15532.5
8	16004.0
9	16768.5
10	17533.0
11	17729.5
12	17926.0
13	17431.5
14	16937.0
15	16067.5
16	15198.0
17	14079.5
18	12961.0
19	12597.0
20	12233.0
21	12541.0
22	12849.0
23	13198.5
24	13548.0
25	16063.5
26	20292.5
27	22006.0
28	26744.0
29	31482.0
30	38465.0
31	45448.0
32	54065.0
33	62682.0
34	69918.0
35	77154.0
36	78010.5
37	78867.0
38	100308.5
39	121750.0
40	162156.0
41	202562.0
42	244666.0
43	286770.0
44	306141.0
45	325512.0
46	320928.5
47	316345.0
48	308483.0
49	300621.0
50	287212.5
51	273804.0
52	243127.5
53	212451.0
54	191470.0
55	170489.0
56	163596.5
57	156704.0
58	150893.5
59	145083.0
60	135297.0
61	125511.0
62	112119.5
63	98728.0
64	85174.5
65	71621.0
66	59696.5
67	47772.0
68	39560.0
69	31348.0
70	27226.0
71	23104.0
72	18849.5
73	14595.0
74	11809.5
75	6416.0
76	3808.0
77	2733.0
78	1658.0
79	1298.0
80	938.0
81	833.0
82	728.0
83	444.0
84	160.0
85	121.5
86	83.0
87	74.5
88	66.0
89	62.5
90	59.0
91	35.5
92	12.0
93	10.0
94	8.0
95	7.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3465243.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.163427169950758
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.36887488734517	14.779376329171296
2	8.236161768972522	2.9919384890133043
3	2.853899043626826	1.5550976228792392
4	1.3637429707354582	0.9908098450994314
5	0.7342999573508137	0.6668701898119725
6	0.5050984726590972	0.5504591593078128
7	0.3500927215493363	0.4451218555413854
8	0.26150341219405193	0.37998385456642203
9	0.1952485572483257	0.3191744654656133
>10	1.8034047169197431	7.422344509601753
>50	0.7127986801506003	9.629965149879919
>100	1.593933581384454	56.113652166006844
>500	0.01678495523428707	1.985880169118293
>1k	0.0038365611964084737	1.2734371292812354
>5k	0.0	0.0
>10k+	3.1971343303403946E-4	0.895889065255427
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17571	0.5070640067666251	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	13284	0.38334973910920533	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	4026	0.1161823283388784	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3788	0.1093141231365304	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.771601010376473E-5	0.0	0.0	0.09745348306020675	0.0
2	5.771601010376473E-5	0.0	0.0	0.38770729787203956	0.0
3	5.771601010376473E-5	0.0	0.0	0.5409721627025869	0.0
4	5.771601010376473E-5	0.0	0.0	0.8114293860488283	0.0
5	5.771601010376473E-5	0.0	0.0	1.295551278799207	0.0
6	5.771601010376473E-5	0.0	0.0	1.8381972057948028	0.0
7	5.771601010376473E-5	0.0	0.0	2.1967002025543376	0.0
8	5.771601010376473E-5	0.0	0.0	3.1016872409813683	0.0
9	5.771601010376473E-5	0.0	0.0	3.4777936208225513	0.0
10	5.771601010376473E-5	0.0	0.0	4.054145697718746	0.0
11	5.771601010376473E-5	0.0	0.0	4.5218473855946035	0.0
12	5.771601010376473E-5	0.0	0.0	4.957083817787093	0.0
13	8.65740151556471E-5	0.0	0.0	5.123652222946558	0.0
14	8.65740151556471E-5	0.0	0.0	5.181368233050323	0.0
15	8.65740151556471E-5	0.0	0.0	5.27928344419136	0.0
16	8.65740151556471E-5	0.0	0.0	5.500999497004972	0.0
17	8.65740151556471E-5	0.0	0.0	5.746523403986387	0.0
18	8.65740151556471E-5	0.0	0.0	6.127449070671235	0.0
19	8.65740151556471E-5	0.0	0.0	6.267179531132449	0.0
20	8.65740151556471E-5	0.0	0.0	6.429505809549287	0.0
21	8.65740151556471E-5	0.0	0.0	6.620228364937177	0.0
22	8.65740151556471E-5	0.0	0.0	6.8095945940876295	0.0
23	8.65740151556471E-5	0.0	0.0	7.030531480764841	0.0
24	8.65740151556471E-5	0.0	0.0	7.19608985574749	0.0
25	8.65740151556471E-5	0.0	0.0	7.327682358784074	0.0
26	8.65740151556471E-5	0.0	0.0	7.460198317982318	0.0
27	1.1543202020752946E-4	0.0	0.0	7.587750700311638	0.0
28	1.4429002525941182E-4	0.0	0.0	7.728231468904201	0.0
29	1.4429002525941182E-4	0.0	0.0	7.8632580745419585	0.0
30	1.4429002525941182E-4	0.0	0.0	8.055048376116769	0.0
31	1.4429002525941182E-4	0.0	0.0	8.21985644296807	0.0
32	1.4429002525941182E-4	0.0	0.0	8.371764981561178	0.0
33	1.4429002525941182E-4	0.0	0.0	8.518450221239895	0.0
34	1.4429002525941182E-4	0.0	0.0	8.666809225211622	0.0
35	1.4429002525941182E-4	0.0	0.0	8.85946526693799	0.0
36	1.4429002525941182E-4	0.0	0.0	9.02932348467337	0.0
37	1.4429002525941182E-4	0.0	0.0	9.191332325034637	0.0
38	1.4429002525941182E-4	0.0	0.0	9.34667496622892	0.0
39	1.4429002525941182E-4	0.0	0.0	9.496650018483551	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAATCG	40	6.8212103E-9	45.000004	9
ACTACGC	40	6.8212103E-9	45.000004	17
GAACGTA	40	6.8212103E-9	45.000004	9
CGTAAGT	20	7.0352555E-4	45.000004	35
ACCGTAC	35	1.212884E-7	45.000004	31
TACGCTA	25	3.8924387E-5	45.0	37
TATTACG	95	0.0	45.0	1
TCGACTA	30	2.1667747E-6	44.999996	2
CGGTCTA	440	0.0	43.465908	31
CGTTTTT	13495	0.0	42.74917	1
ACGTGCG	70	0.0	41.785717	1
CGACGGT	470	0.0	40.69149	28
ACAATCG	50	1.0822987E-9	40.5	1
CTTACGT	50	1.0822987E-9	40.5	24
ATGCTCG	45	1.9303116E-8	40.000004	1
GTCGCAA	80	0.0	39.375004	44
ATTGCGG	785	0.0	38.980892	2
CACGACG	505	0.0	38.76238	26
GCGCGAC	1215	0.0	38.703705	9
CTACAGT	1780	0.0	38.679775	28
>>END_MODULE